植物表達載體 的英文怎麼說

中文拼音 [zhíwùbiǎodázǎitǐ]
植物表達載體 英文
plant expression vector
  • 植 : Ⅰ動詞1. （栽種） plant; grow; cultivate 2. （樹立） establish; set up Ⅱ名詞（姓氏） a surname
  • 物 : 名詞1 （東西） thing; matter; object 2 （指自己以外的人或與己相對的環境） other people; the outsi...
  • 表 : Ⅰ名詞1 （外面;外表） outside; surface; external 2 （中表親戚） the relationship between the child...
  • 達 : Ⅰ動詞1 （暢通） extend 2 （達到） reach; attain; amount to 3 （通曉; 明白） understand thoroughly...
  • 載 : 載Ⅰ名詞（年） year : 一年半載 six to twelve months; six months to a year; 三年五載 three to five ...
  • 體 : 體構詞成分。
  • 植物 : plant; flora; botany; stray; greenery; phyton; phytum; phyta; phyt ; phyto ; phyte : 草本植物 her...
  • 表達 : deliver; express; show; voice; convey; communicate
  • 載體 : [化學] carrier; supporter; isotopic carrier
  1. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  2. ( 2 ) at translation level plant mutual sequence of starting translation aaca was added to start codon of t - pa gene by pcr ampliation and plant expression vector pbet was constructed

    （ 2 ）在翻譯水平上通過pcr擴增的方式在t - pa基因起始密碼子處添加了植物翻譯起始共有序列aaca ，構建了植物表達載體pbet 。
  3. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  4. Cloning of monomial promoter of actin1 gene and construction of plant expression vector

    1的克隆及植物表達載體的構建
  5. Construction of an expressing vector for antisense rna - ribozyme chimeric dna sequence against tomato acc synthase gene and transformation of tomato

    核酶嵌合基因植物表達載體的構建及對番茄的轉化
  6. Deduced amino acid sequence of s1, s2, pvin were also highly homologous each other ( 98 %, 99 % in each case ). the stilbene synthase genes were excised from the plasmids by bamh i and sac i digestion and intergrated into a binary vector, pbi121 and pev2, from which the p - glucuronidase ( gus ) gene sequence had been removed by the same digestion to prepare a 35s promoter - stilbene synthase 2 - nopaline synthase polyadenylation site construct and a tfp2 promoter - stilbene synthase 1 - nopaline synthase polyadenylation site construct. the recombinant plasmids were called pbs2, pev2s 1. respectively

    用bamhi和saci同時酶切ps2 （ s2表示來自雷司令的芪合酶基因） 、 ps1 （ s1表示來自粉紅玫瑰的芪合酶基因）以及pbi121 、 pev2 ，使得s2 、 s1分別插入替代pbi121 、 pev2中的gus基因，構建成植物表達載體pbs2 、 pev2s1 ， pbs2中含camv35s組成型啟動子，使s2基因能在番茄植株的各個部位表達； pev2s1則含有果實特異性啟動子tfp2 ，使s1基因只在番茄果實中表達。
  7. As a popular vegetable, tomato is rich in vatamin a, b, p and other nutrients ; among them, lyxopene can prevent prostate gland tumor. so introducing stilbene synthase gene into tomato to get new health - protection tomato will have important social and economic effect. our research is made up of four parts ; the cloning and sequencing of stilbene synthase ; tomato genetic transforming mediated by agrobacterium - lba4404 ; quantification of resveratrol in transgenic tomato by hplc analysis

    本研究包括四個方面的內容：芪合酶基因（ stilbenesynthasegene ）的克隆與全序列分析；芪合酶基因（ stilbenesynthasegene ）植物表達載體的構建；農桿菌lba4404介導的番茄遺傳轉化： hplc技術分析轉基因植株中表達白藜蘆醇的含量。
  8. Leaves of tobacco ( nicotiana tabacum ) were wounded, infected by agrobacterium tumefaciens strain lba4404 and gv3101 : pmp90 harboring expression vector pbgsb and pbglsb and co - cultured for 3 days in darkness on the culture medium ( ms + 0. 5mg / l 6 - ba + 0. 7 % agor ph 5. 7 )

    4以煙草（品種sr ）無菌苗葉片為外植體，採用農桿菌浸染的葉盤轉化法，用構建好的植物表達載體對煙草進行遺傳轉化。外植體置於無抗生素的誘芽培養基（ ms 0
  9. This gene, artificially put under the control of camv 35s, was introduced into tobacco with the aid of agrobacterium, and the transgenic plants obtained were identified by gus staining, pcr and southern blot analysis

    將arge與組成型啟動子camv35s相連，構建植物表達載體，通過農桿菌介導轉化煙草。經過gus染色、 pcr及southern鑒定獲得了轉基因植株。
  10. Construction and transformation of inverted repeat of turnip mosaic virus coat protein gene

    基因反向重復植物表達載體的構建及遺傳轉化
  11. Green fluorescent protein ( gfp ) gene was conjugated to the 3 " end of the pap gene in order to screen easily of the transgenic cotton plants. the combined gene was cloned into plant expression vector pbi121 and then transformed. about 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays

    為了便於轉基因棉花後代的篩選，在pap基因的3 』端融入了綠色熒光蛋白gfp ）基因，然後將融合基因克隆在植物表達載體pbi121上，再進行遺傳轉化，得轉基因棉花種子5000餘粒，將種子播種長到于葉展開時，先在黑暗中用紫外燈照射，查找表現綠色熒光的幼苗，然後再用地高辛（ dig ）標記的pap基因特異性探針對這些棉花進行點雜交，最後發現有8株棉花表現陽性反應，說明pap基因的確己經轉到了棉花的基因組中，其棉花黃萎病的抗性鑒定正在進行之中。
  12. Plant defensin afp ( 238bp ) was amplified with six long complementary primers after two rounds of pcr amplification and plant expression vector peafp was constructed. 3

    通過合成六條長鏈引物，經兩次pcr擴增，獲得了238bp的植物防禦素afp基因全長，並構建了植物表達載體peafp 。
  13. 2. obtaining of mcema ( modified cema ) and plant defensin afp gene mcema ( 141bp ) was amplified with pucspcema plasmid as template and p5, p4 as primers, and plant expression vector pemcema was constructed

    Mcema和植物防禦素基因的pcr合成以經測序驗證的spcema為模板，用p _ 5和p _ 4擴增得到了全長141bp的mcema ，並構建了植物表達載體pemcema 。
  14. The resulting plasmid, named prok - sod2, was mobilized to agrobacterium tumefaciens strain gv3101 used for plant transformation. the yeast sod2 gene was introduced into arabidopsis thaliana ( ecotype landsberg erecta ) by agrobaterium tumefaciens - mediated transformation with floral - dipping method under the control of camv 35s promoter. transformants were selected for their ability to grow on medium containing kanamycin ( 30mg / l ), several homozygous lines that were all tolerant to kanamycin were selected and used for further molecular and physiological determination

    本實驗將sod2基因構建到植物表達載體prok中，導入農桿菌后，進行植物遺傳轉化，實現其在擬南芥中過量表達，在含30mg l的卡那黴素的培養基上篩選獲得純合轉基因株系，自交一代獲得足夠的純和轉基因種子后，對其進行了分子生物學的驗證及生理指標的檢驗。
  15. ( 3 ) at post - translation level plant mutual sequence of starting translation aaca and eukaryotic secretory signal peptide sequence was added to 5 ' - flanking region of t - pa gene and kdel ( sequence located to endoplastic reticulum ) to 3 ' - flanking region by pcr amplication and plant expression vector pbemt was constructed

    （ 3 ）翻譯后水平通過pcr擴增的方式在t - pa基因5端添加了真核分泌信號肽序列和植物翻譯起始共有序列aaca ，在3端添加了內質網定位序列kdel ，構建了植物表達載體pbemt 。
  16. Construction of plant expression vector with human endostatin gene and transformation of tobacco

    利用人內皮抑素基因構建植物表達載體及對煙草的遺傳轉化
  17. Construction of vp1 plant expression vector of foot - and - mouth disease virus

    1植物表達載體的構建
  18. The aim of this research is to establish a transgenic system for flower plants and investigate the functions of mapkk in transgenic plants in the future

    本研究構建了野生型、突變型mapkk基因植物表達載體，並用凍溶法將其導入了根癌農桿菌lba4404 。
  19. Ssmapkk transformations were screened on media with kanamycin ( 30mg / l ). nineteen individual kanamycin resistant plants were obtained. t2 plants were checked for integration of foreign gene by counting ratio of the number of tolerant plants to the number of non - tolerant plants on selection medium with kanamycin ( 30mg / l )

    將ssvp和ssmapkk的全長cdna分別克隆入植物表達載體pcambia1300和prok中，導入根瘤農桿菌gv3101后，由花浸泡法進行擬南芥遺傳轉化，轉化ssvp鹽地堿蓬ssop和ssmapkk基因的克隆與功能鑒定的擬南芥在含潮黴素（ 25mg ）的ms培養基上篩選，獲得t ；代轉基因植株。
  20. A binary plant expression vector with osg6b " driving report gene gus was constructed and transferred into tobacco via agrobacterium tumefaciens mediation

    將克隆的啟動子與報告基因gus相連，構建植物表達載體，通過農桿菌介導轉化煙草。
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