植黴素 的英文怎麼說

中文拼音 [zhíméi]
植黴素 英文
phytomycin
  • : Ⅰ動詞1. (栽種) plant; grow; cultivate 2. (樹立) establish; set up Ⅱ名詞(姓氏) a surname
  • : Ⅰ形容詞1 (本色; 白色) white 2 (顏色單純) plain; simple; quiet 3 (本來的; 原有的) native Ⅱ名...
  • 黴素 : mycin
  1. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到物表達載體pbi121中,通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中,然後採用葉盤法,在該農桿菌的介導下將pap基因導入普通煙草中,經過卡那抗性篩選,最後獲得了轉pap基因的工程煙草株,摩擦接種煙草花葉病毒( tmv ) ,與非轉基因煙草相比,能夠推遲癥狀表現達2月之久,說明pap基因能夠在其它物體內產生有活性的高抗病毒的蛋白質。
  2. The results also revealed that it was a reasonable choice to use carbenicillin as the antibiotics of inhibiting growth of remnant agrobacterium after co - culture, and when hygromycin was used as the selective agent, continuous selection at low concentrations ( 50 ~ 150mg / l ) produced the highest numbers of transgenic plants without escapes

    試驗表明,在農桿菌介導高羊茅遺傳轉化中,抑菌劑宜選用梭節青霉;以潮作為選擇劑時,採用低濃度( 50一15om留l )連續篩選的方式比較合適,在該方式下,獲得的轉基因株較多。
  3. A number of kanamycin resistant transformants were obtained two months later. they were morphologically normal, but grew slower comparing with the control tobacco

    2個月後獲得了一批卡那抗性煙草株,抗性株大田生長表型正常,生長速度相對緩慢。
  4. The tl seeds are screening on the ms medium which contains 50ug / ml kan, and there are about 70 % of the transgenic lines showed the kan - resistance with a ratio of 3 : 1. however, the other lines did n ' t show the ratio consistent with the mendelism. it might prove that most of the transgenic plants only have one insert

    對收獲的轉基因番茄t _ 1代種子進行卡那抗性篩選,結果發現, 70以上獨立轉化株系的t _ 1代種子出現了3 1的抗性分離,另一些獨立轉化株系的種子並未表現出符合孟德爾遺傳的分離比,說明大部分er - shsp基因是以單拷貝的方式插入物基因組。
  5. Agobacterium tumefaciens strain a311 carrying the plant tranfer vector pb1121, which contains the neomycin phosphotransferasell gene ( nptll ) and p - glucuronidase reporter gene ( gus ) both under the control of the camv 35s promoter, was used in the establishment of the genetic tranformation of white clover

    選用苗齡4 5天的帶柄子葉作為外體,先將外體預培2天,再與根癌農桿菌a311共培養3 4天後,轉入附加有40mg l ~ ( - 1 )卡那和400mg
  6. The regeneration system of soybean cytoledon node and agrobacteriunr mediated transformation method is the first selection at present. in the second part of this experiment, the expression vector prok2 containing npt ii and ssnhx1 ( na + / h + antiporter ) gene from suaeda salsa was introduced into soybean cytoledon nodes by gene transformation mediated by agrobacterium tumefaciens, and kanamycin resistant transgenic p lants were obtained by screening in selective condition

    本實驗第二部分通過農桿菌介導法將含npt -和鹽地堿蓬na ~ + h ~ +反向轉運蛋白基因( ssnhx1 )的表達載體prok2導入大豆子葉節中,經過含km的篩選培養基連續篩選,獲得了ssnhx1轉基因株,篩選劑卡那的適宜濃度是50mg . l ~ ( - 1 ) 。
  7. The resulting plasmid, named prok - sod2, was mobilized to agrobacterium tumefaciens strain gv3101 used for plant transformation. the yeast sod2 gene was introduced into arabidopsis thaliana ( ecotype landsberg erecta ) by agrobaterium tumefaciens - mediated transformation with floral - dipping method under the control of camv 35s promoter. transformants were selected for their ability to grow on medium containing kanamycin ( 30mg / l ), several homozygous lines that were all tolerant to kanamycin were selected and used for further molecular and physiological determination

    本實驗將sod2基因構建到物表達載體prok中,導入農桿菌后,進行物遺傳轉化,實現其在擬南芥中過量表達,在含30mg l的卡那的培養基上篩選獲得純合轉基因株系,自交一代獲得足夠的純和轉基因種子后,對其進行了分子生物學的驗證及生理指標的檢驗。
  8. Rapamycin is indicated as an immunosuppressant for organ rejection in patiens receiving transplantations

    研究顯示,雷帕可能是接受移手術病人器官排斥的免疫抑制劑。
  9. Determination of sterigmatocystin in vegetable foods

    物性食品中雜色麴的測定
  10. Ssmapkk transformations were screened on media with kanamycin ( 30mg / l ). nineteen individual kanamycin resistant plants were obtained. t2 plants were checked for integration of foreign gene by counting ratio of the number of tolerant plants to the number of non - tolerant plants on selection medium with kanamycin ( 30mg / l )

    將ssvp和ssmapkk的全長cdna分別克隆入物表達載體pcambia1300和prok中,導入根瘤農桿菌gv3101后,由花浸泡法進行擬南芥遺傳轉化,轉化ssvp鹽地堿蓬ssop和ssmapkk基因的克隆與功能鑒定的擬南芥在含潮( 25mg )的ms培養基上篩選,獲得t ;代轉基因株。
  11. The methods of evans and martin were changed slightly and used to isolate the mouse es cell in my experiment. in brief, the intact blastocysts were plated on sto feeder layer treated with mitomycin, and were cultured in the media supplymented with brl condition medium

    聯合evans和martin的方法,稍加改良來分離小鼠胚胎幹細胞,把昆明白小鼠完整的囊胚直接種在經絲裂滅活的sto飼養層細胞上,在含有brl條件培養基的es細胞培養液中培養。
  12. Gottsch jd. liu sh, minkovitz jb. et al. autoimmunity to a cornea - associated stromal antigen in patients with mooren ' s ulcer. invest ophthalmol vis sci, 1995, 36 : 1541

    王錚.陳家祺,陳龍山,等.板層角膜移聯合環孢a滴眼治療蠶蝕性角膜潰瘍.中國實用眼科雜志, 1996 , 14 : 283
  13. The seedlings were then growing on the medium for 20 days. the primary results show that the tolerable concentration of kanamycin to the tested seedlings is up to 15ug / ml and the suggested concentration for selection of resistant plant is 25ug / ml

    為篩選卡那抗性的轉基因株,還進行了甘藍型油菜卡那敏感性實驗。將正常甘藍型油菜種子表面消毒后,接種于不同濃度卡那ms瓊脂培養基上,光照培養、篩選。
  14. 6. transformation system of mustard a serials of kanamycin concentration was added to optimum medium to test the explants resistance capacity of two kinds of mustard. the transformation procedures described were derived from numerous regeneration and trasformation designed to test factors that might affect shoot regeneration, which including length of co - cultivation. those producing the best result parameters were described as below : after the mustard explants were precultured on regeneration medium for 2 days. they were inoculated with agrobacterium for 20 minutes. inoculated explants were co - cultivated for 4 days and in shadow at first 2 days. then transferred to the same medium plus 30 mg / l kanamycin and 500mg / l garb. all of them were transferred to fresh medium every 2 weeks. the kan - resistant plants were regenerated

    芥菜外體高頻遺傳轉化體系的建立在最適培養基上試驗了兩類芥菜的三種外體對卡那的敏感性、預培養天數、浸菌時間等因的影響,建立了芥菜高頻轉基因再生體系:取生長4天的芥菜子葉、下胚軸和25天的葉片在分化培養基上( ms + ba3 . 0mg / l + naa0 . 1mg / l )預培養2 - 3天後,投入農桿菌菌液中浸染20分鐘,在分化培養基上暗培養2天,正常條件下培養2天後,轉入抗性培養基( ms ba3
  15. We obtained our transgenic material, the rice suspension cells, by inducing embryonic rice callus. then we constructed the expression vector pca - ced9, and transferred ced - 9 gene into the rice callus and embryogenic suspension cells. the work of using hygromycin selective medium to obtain regenerated plants is still going

    構建了用於水稻中表達的載體pca - ced9 ,通過農桿菌eha105轉化導入水稻愈傷組織和水稻懸浮細胞,經潮( hym )篩選,以期望獲得抗性株,目前該工作仍在進行中。
  16. Organ transplants did not become routine until the 1980s with the approval of a new drug, cyclosporine, which prevented rejection without destroying the body ' s resistance to infection

    器官移直到二十世紀八十年代隨著一種新藥環胞的發明才普遍使用,這種藥物不會破壞身體的免疫系統又能防止排異。
  17. Experimental study of implantable controlled release mitomycin c on hepatocellular carcinoma in nude mice

    入式絲裂控釋劑的抑瘤作用
  18. The predicted bands were amplified from both to and ti generation transgenic plants by pcr, indicating that the hygromycin resistance gene ( hpt ) and svde gene were both transferred into tobacco

    Pcr擴增潮抗性基因hpt和svde基因結果顯示,在『轉淬回株t斤t ;代中都分別擴增出互okb和二
  19. 89 ) via agrobacterium mediation. pcr and southern blotting analysis showed that the cmo and badh genes were integrated into tobaccos " genome. betaine accumulation in transformed tobaccos was higher than that in control plants

    89 ) ,獲得卡那抗性和潮抗性株, pcr和southern雜交均證明外源cmo和badh基因大連理工大學博士學位已整合到煙草基因組中。
  20. When the seeds from to transgenic plants were cultured on medium supplemented with 50mg / l hygromycin, the ratio of germinated to non - germinated seeds is 3 : 1, which is consisted with mendel ' s first law. southern blotting demonstrated that the svde gene had been integrated into the genome of tobacco plants

    轉基因株的l代種了在潮培養基上的萌發數與未萌發數的比值為3 : l ,符合單基因的盂德爾分離規律。
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