標記分析物 的英文怎麼說

中文拼音 [biāojìfēnxīwù]
標記分析物 英文
labelled analyte
  • 標 : Ⅰ名詞1 [書面語] （樹梢） treetop; the tip of a tree2 （枝節或表面） symptom; outside appearance; ...
  • 記 : Ⅰ動詞1 （把印象保持在腦子里） remember; bear in mind; commit to memory 2 （記錄; 記載;登記） writ...
  • 分 : 分Ⅰ名詞1. （成分） component 2. （職責和權利的限度） what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] （料想） judge
  • 析 : Ⅰ動詞1. （分開; 散開） divide; separate 2. （分析） analyse; dissect; resolve Ⅱ名詞（姓氏） a surname
  • 物 : 名詞1 （東西） thing; matter; object 2 （指自己以外的人或與己相對的環境） other people; the outsi...
  • 標記 : （標志; 記號; 貨物標記） tab; sign; stamp; peg; label; mark; flag; blip; notation; fleck; track; ...
  1. Dsmv is proved as the predominating virus - pathogen on aroid plants from zhejiang province and other regions in china. cdna of dsmv rna 3 " end partial sequence and subgenomic rna promoter region of cucumber mosaic virus ( cmv ) rna3 were used as probes for detection of dsmv and cmv respectively. total rna extracted from field samples were used for rna dot - hybridization

    用侵染馬蹄蓮的dsmv3末端序列和黃瓜花葉病毒（ cmv ）的亞基因組啟動子區互補dna序列為標記探針，對自然感病的天南星科植物進行rna斑點雜交，並結合雙鏈rna分析、病毒提純和形態學觀察，對杭州等地16屬天南星科植物的81個樣品進行了病毒鑒定。
  2. Our previous studies showed existence of apoplast cam in the plant cell and cam had many extracellular functions. so it supposed cam may be one of important extracellular polypeptides and trigger the intracellular signal transduction by binding the receptor. in this study, radiolabelled ligand is used to investigate the binding characteristic of cam and a. thaliana protoplasts. and chemical crosslinking is employed to explore binding proteins in the membrane. at first, ( 35 ) ~ s - cam was produced using ( 35 ) ~ s - labeled amino acid mixture in e. coli. sds - page and autoradiograph indicated high - purified, high - specific radioactivity ( 35 ) ~ s - cam was obtained. electrophoresis of ( 35 ) ~ s - cam is the same as that of unlabeled cam with ca ~ ( 2 + ) or egta ; a quatitive of protoplasts was prepared by enzymolysis

    首先，用~ （ 35 ） s標記的氨基酸混合物喂養工程菌成功地制備了~ （ 35 ） s標記的擬南芥鈣調素（ ~ （ 35 ） s - cam ） ， ~ （ 35 ） s - cam純度高、放射活度高、 ca ~ （ 2 + ）與egta存在時的電泳行為與未標記cam相同，可作為一種高靈敏性的探針用於進行受體學分析實驗；用擬南芥種子誘導愈傷，通過酶解制備了大量原生質體。
  3. Two hundred and seventy - four sites were detected by 15 10 - mer primers, out of which 262 were polymorphic ( 95. 6 % of the total loci detected ). all of the materials were clearly distinguished, of which fourteen samples have forty - five typical rapd bands. before the molecular taxonomy was introduced, people never doubted subgenus yulania ' s disrelation with subgenus magnolia and a close relation between subgenus yulania and michelia, as there is in both genera a sameness seen in their prolepsis and powder chambers dehiscing on the side

    3 、通過對玉蘭亞屬rapd擴增譜帶的分析，發現該類群具有很高的多態性：多態位點百分率高達95 . 6 ，產生的多態性帶數與所用引物數的比例為17 . 6 ， 15條隨機引物清楚地分開了31個種類，其中的14個種共有45條特徵性帶，在利用rapd標記已研究過的類群中，本類群多態性較高。
  4. The purposes of our work are to establish a simplified method of multiplex pcr based on chimeric primers for str loci, to develop a set of fluorescent quadriplex str system for forensic dna typing based on this method, and to validate the forensic application of the system under the guidelines of tmgdam ( the technology working group on dna analysis methods ) in order to address concerns presented in today ' s legal environment

    目的本課題旨在探索一種新的str基因座復合擴增方法，我們稱為嵌合引物str復合擴增法。應用熒光標記毛細管電泳激光自動檢測技術平臺，建立一套新的法醫str基因座復合擴增體系，並按照美國dna分析方法技術工作組（ thetechnologyworkinggroupondnaanalysismethods ， twgdam ）的指導方案進行法醫學實用性研究。
  5. As analyzed, ( 1 ) the rapd technique is highly sensitive to investigating genetic diversity in t. lepturus and e. muticus. t. lepturus exhibits lower polymorphism and genetic diversity than e. muticus ; ( 2 ) according to the analysis of the partial mitochondrial 16s rrna gene sequences, a very low intraspecific variation and considerably high divergence among species were found, which reveals a dual nature of conservatism and variability in mitochondrial 16s rrna gene ; ( 3 ) five primers generate the species - speeific rapd sites and these sites can be served as the molecular markers for species identification and ( 4 ) it can be proved at dna variation level that t. lepturus and e. muticus are of two species respectively pertainiag to different genera, which supported the nelson taxonomic conclusion

    分析結果表明: （ 1 ） rapd技術研究黃海帶魚和小帶魚的遺傳多樣性具有較高的靈敏度和檢出率，帶魚的多態比例和遺傳多態度均較小帶魚的低; （ 2 ）線粒體165出兇a基因序列在分析兩物種遺傳變異時表現出保守和變異的雙重特性，種內變異極小而種間較大: （ 3 ） 5個隨機引物擴增出種特異的ra衛d帶，可作為種間分子鑒定標記; （ 4 ）研究證實帶魚和小帶魚是不同屬的兩個種，從而在分子水平上支持了nelson分類系統的觀點。
  6. In summary, we should investigate the more efficient cultural system for mammary orgnoids in vitro for enriched more mammary epithelial stem cells, and to screen the expression profiles in mammary epithelial stem cells to discover the specific marker for mammary epithelial stem cells in order to utilize the model of mammary epithelial stem cells to regenerate transgenic mammary glands in cow or sheep for human health, to uncover the mechanism of organogenesis, and differentiation

    在此之前，仍然只能以造血幹細胞的特異性表達抗原scal - 1作為一個標記，進行類乳腺幹細胞的分析和分選研究。進一步研究類乳腺幹細胞的特異性表達譜，以期能夠找到類乳腺幹細胞的特異性表達抗原，分離得到類乳腺幹細胞，作為發育生物學和再生生物學研究的模型。
  7. In this work, some species of palmae cultivated in the xiamen botanical garden had been selected to be analyzed their genetic diversity with rapd ( random amplified polymorphic dna ) technique. according to the result of genomic dna amplified with pcr ( polymerase chain reaction ), genetic distance and similarity between different palm species were calculated on nei " s estimate of similarity and genetic distance. a primary but first time research at the phylogenetic relationships of some genera and species, the molecular classification and identification of some puzzling species of palmae was carried out through upgma ( unweighted pair group mean average ) cluster analysis of the genetic distance together with comparative study of the morphological structure characteristics

    本文在初步調查分析了棕櫚科植物在我國的自然分佈、引種馴化情況以及該類植物在廈門地區栽培應用狀況的基礎上，首次採用rapd分子標記技術，對廈門萬石植物園引種的一些棕櫚科植物的遺傳多樣性進行了研究，根據pcr對基因組dna擴增的結果，用nei ' s相似性系數計算了不同植物間的遺傳距離和遺傳一致度，通過對遺傳距離的upgma聚類分析，並結合形態分類的特點，對棕櫚科植物的屬、種間的系統分類關系和一些疑難種的分類鑒定進行了初步研究。
  8. The experimental method includes selecting pure complexes of histidine - containing or cysteine - containing materials, from c - and n - terminal group of these amino acids to link to a group which have color or fluorescence or ultraviolet absorption, elucidating their binding affinity, fluorescence or uv - visible spectrum properties with zinc at physiological concentration and to elucidate their structure in the solid state via infrared spectroscopy. with the help of the concerned the data, the analysis was done to prove whether it can be applied to the zinc detection, in other words, whether it can be used as a new fluorescence probe for zinc detection

    本實驗首次選用在生物體內與zn ~ （ 2 + ）鍵合能力很突出的物質? ?組氨酸和半胱氨酸，採用類似於多肽合成的方法，在其羧基或氨基分別嫁接上一個帶有標記的基團，生成穩定的共價鍵化合物；在此化合物中模擬生理濃度條件加入鋅離子，通過紅外圖譜、紫外圖譜或熒光圖譜的變化分析鋅離子對標記基團是否產生影響，再結合有關數據分析其是否適合檢測鋅離子，即是否可能作為新的鋅離子熒光探針。
  9. Phylogeny analysis is performed with phylip software package and encoding sequence of bdnf gene. the phylogeny trees have been drawn with three different methods ( maximum parsimony method, genetic distance method and maximum likelihood method ), respectively. the analysis outcomes are not all consistent for the reason that it is closely related to the selected methods and the conservative level of the sequences

    採用不同的統計學分析方法，最大簡約法（ maximumparsimonymethod ） 、最大似然法（ maximumlikelihoodmethod ）和遺傳距離法（ geneticdistancemethod ） ，得到了物種系統發育進化樹，但拓撲結構並不完全一致，這是可能是因為分子系統學研究與採用的分析方法和所選基因的保守程度即作為分子標記的可信度密切相關。
  10. Application of microsatellite dna polymorphisms and dna fingerprints to inbred strain mice and rats to screen the exact, dependable, particular genetic monitoring marker method of laboratory animal, the author had studied the application of microsatellite dna polymorphisms and dna fingerprints to inbred strain mice and rats, and compared the two methods with the biochemical marker enzyme method. the study had established the foundation of the molecular genetic monitoring marker method of laboratory animal

    本文通過對dna指紋技術和pcr擴增微衛星dna技術在近交系大、小鼠遺傳檢測中的應用研究，並與生化位點標記分析法進行比較，旨在篩選出具有精確、可靠、特異性好的實驗動物遺傳檢測方法，為建立分子生物學實驗動物遺傳質量監測技術和標準奠定基礎。
  11. Although this method gives high sensitivity, the radioactive labels present many problems such as a potential hazard to analyst and environment, which limited its application in dna diagnostic laboratories. in order to overcome these problems a serious of non - radioactive dna probes such as fluorescent, chemiluminescent and electrochemical probes have been developed. although these new methods display many advantages, they have not been used to take place completely the traditional method because of low sensitivity or complex equipment or other shortcomings

    自20世紀80年代以來，各種非同位素如酶、熒光素、生物素、地高辛標記的化學發光法和熒光分析法以及以電活性物質做標記的電化學方法相繼問世，這些方法雖然在一定程度上克服了同位素標記的缺陷，但由於存在靈敏度不夠高或檢測系統龐雜或儀器價格昂貴或標記物不穩定等缺陷，還不能完全取代傳統方法。
  12. Stable fluorescent labels bhhct - eu3 +, bpta - tb3 ", and europium nanoparticle were used to develop sandwich - type time - resolved fluoroimmunoassays for hbsag. fluorescence intensity was directly detected on the surface of the solid phase

    利用穩定的稀土標記物bhhct - eu3 + 、 bpta - tb3 +和銪納米顆粒建立夾心型固相時間分辨熒光免疫分析（ trfia ）直接定量測定乙肝表面抗原（ hbsag ）的新方法。
  13. The amplification system was optimized so that the pcr with different primers can be carried out under the same condition. the pcr products were sequenced using sanger ' s terminator and fluorescent label techniques. sequences were analyzed and compared base on sequencing analysis3. 4 and seq / ede software

    方法根據mtdna控制區及其周圍區域的序列，設計多對引物，探索優化擴增體系，使擴增條件能夠同時滿足多對引物的需要，用sanger末端終止法及熒光標記技術對樣本進行dna測序， sequencinganalysis3 . 4和seq ede軟體進行序列分析和比對。
  14. P - galactosidase of e. coli which is scarce in human blood plasma and has a variety of substrates, is one of the most frequently used enzyme labels. it is used both in heterogeneous and homogeneous enzyme immunoassay

    大腸桿菌的-半乳糖苷酶，因人血漿中缺乏此酶，並具有大量易得的底物，被用於均相及非均相免疫分析，是目前最常用的標記酶之一。
  15. In this paper, it were briefly introduced principle and structure. the application of microsatellite technique was reviewed in the study of animal, for instance, construction of genetic linkage map, molecular marker assisted breeding, evaluation of genetic diversity, genetic monitoring and so on were summarized

    本文簡述了微衛星技術基本原理、優點和檢測方法以及在實驗動物科技等領域中的應用，例如遺傳連鎖圖譜、分子標記輔助育種、遺傳多樣性分析、遺傳質量檢測等。
  16. The objective of the present paper was to investigate the genome constitution of three species of hystrix and their taxonomic status from cytological and molecular data. by rapd and issr assay, the present study was to evaluate phylogenetic relationships among hystrix, leymus and psathyrostachys, and to compare the use of two molecular markers in the genus and species of triticeae

    本研究（ 1 ）通過形態學特徵比較、細胞學和繁育學資料，探討三個hystrix物種的染色體組組成及親緣關系； （ 2 ）通過rapd分析和issr分析，探討hystrix與leymus 、新麥草屬psathyrostachys的屬間親緣關系； （ 3 ）比較rapd和issr兩種dna分子標記在hystrix及近緣屬系統分類研究中的應用。
  17. A mixture of three amino acids ( arg, gly, glu ) labeled with fluorescein isothiocyanate ( fitc ) was separated in pdms microfluidic chip, the separation voltage is 200v / cm, the separation time is less than 120 seconds ; according to ccd fluorescence images, two distinct physical processes - stacking and destacking during sample injection were studied qualitatively ; rhodamine b, a kind of temperature - dependent fluorescence dye, was used as probe to develop a temperature - fluorescence intensity equation, then temperature - color map in microchannels was constructed, and temperature trait in microchannels on the pdms microfluidic chip was analysed. according to the results, we conclude that the electric field applied to the pdms microfluidic chip should not exceed 400v / cm

    利用pdms微流控晶元對fitc標記的精氨酸、甘氨酸、谷氨酸混合物進行了電泳分離，分離電壓為200v cm ，分離時間不到120秒；通過拍到的熒光顯微圖像對電泳注樣過程中復雜的樣品分子積聚與解聚現象作定性的分析；以熒光染料rhodamineb為溫度熒光探針，建立了pdms微流控晶元上的溫度-熒光強度的關系公式，並利用matlab圖像處理工具箱構建出微流體溝道內的溫度色圖，對pdms微流控晶元的微流道溫度特性進行了分析，根據實驗結果，我們認為對于pdms微流控晶元來說，在進行需要外加電場作用的試驗時，外加電場不應超過400v cm 。
  18. Then the effect of turning channel has been studied. 7. this detection system has been used to dna segments separation experiments ; the results showed that it has met the need for dna separation

    將該微通道電泳晶元系統應用於不同熒光標記寡核苦酸、 dna片段的分離分析，實現了寡核著酸的單堿基分辨，同時應用於結核桿菌基因組pcr產物的分析，實驗結果表明此系統可以實現dna片段的快速高效分析。
  19. The analysis of genetic diversity of 10 families in p. taiwanensis hayata. was carried out by rapd molecular markers, which may provide the theoretical base for breeding. 17 primers were screened from 206 random oligonucleotide primers and 38 polymorphic loci were obtained from pcr of 17 primers

    利用rapd分子標記分析了黃山松10個家系的遺傳多樣性，為育種提供依據，從206個隨機引物中篩選出17個引物，獲得38個多態位點。
  20. Dna fingerprints of 13 colonies of 5 strains, including 5 balb / c, 2 balb / c - nu / nu > 4 c51 1 cba / n and 2 dba / 2 from 7 factories of laboratory animal in the beijing and xi ' an areas, were studied with jl - 2 mulilocus probe and southern hybridization and the author compared the dna fingerprint with the biochemical marker enzyme method. it indicated that the fingerprint had 17 - 22 distinguishable fragments and these fragments had high polymorphisms. the fingerprints of balb / c and balb / c - nu / nu, which were abnormal at the hbb site with the biochemical marker enzyme method, was different from their normal groups

    實驗採用jl - 02多位點探針對北京和西安地區較大的7家實驗動物生產供應單位的5個balb c群， 2個balb c - nu nu群， 4個c _ （ 57 ）群， 1個cba n群和1個dba 2群近交系小鼠進行了dna指文圖分析，並與常規生化標記分析法進行了比較，結果顯示：所產生的dna指紋圖的圖帶數均在17 - 22條，具有良好的多態性。
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