標記探測劑 的英文怎麼說
中文拼音 [biāojìtàncèjì]
標記探測劑
英文
labelled probe- 標 : Ⅰ名詞1 [書面語] (樹梢) treetop; the tip of a tree2 (枝節或表面) symptom; outside appearance; ...
- 記 : Ⅰ動詞1 (把印象保持在腦子里) remember; bear in mind; commit to memory 2 (記錄; 記載;登記) writ...
- 探 : Ⅰ動詞1 (試圖發現) try to find out; explore; sound 2 (看望) call on; visit; see 3 (向前伸出)...
- 測 : 動詞1. (測量) survey; fathom; measure 2. (測度; 推測) conjecture; infer
- 劑 : Ⅰ名詞1 (藥劑; 制劑) a pharmaceutical or other chemical preparation 2 (某些有化學作用的物品) a...
- 標記 : (標志; 記號; 貨物標記) tab; sign; stamp; peg; label; mark; flag; blip; notation; fleck; track; ...
- 探測 : survey; search; sound; probe; sounding; detection; acquisition; reconnaissance; localization; fin...
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7. at the first time, the reporter dye, fam was linked to the 5 " - end of the oligonucleotides of the probes, and the tamra was located at the 3 " - end as quencher dye. we use camv35s and fmv promoter, nos terminater, mark gene nptii, and aim gene pat, epsps and cryla ( b ) genes as target sequences, design pairs of sp
7 、首次以fam熒光素標記探針5 』端作為發光基團,以tarma標記探針3 』端為淬滅基團,以camv35s 、 fmv啟動子、 nos終止子、標記基因nptll 、抗除草劑基因epsps 、 pat 、抗蟲基因cry1a )為檢狽目標,設計、篩選出特異性引物和探針,優化實驗參數,建立了轉基因植物通用性熒光pcr定性檢測方法體系。In order to study the expression of 3 - defensins in liver as acute phase response proteins, a murine systemic acute phase responsive model was established by intraperitoneal injection o f lipopolysaccharide ( lps ) in our study. the mbd3 cdna sequence ( 145 - 169 bp ) was labled with [ - 32p ] atp as a probe to detect mbd3 mrna in different tissues by northern blot and analyze the time - and dose - dependant expression caused by lps. the 5 " flanking sequence ( - 167 - 179 bp ) of the mbd3 gene was designed as the probe and labled with [ - 32p ] dctp to investigate the binding of transcriptional factors to this region by electrophoretic mobility shift assay ( emsa ) and south - western blot
以小鼠mbd3基因145 ? 169bpcdna序列合成探針,經[ - ~ ( 32 ) p ] atp標記后通過northernblot方法檢測mbd3在肝臟中的表達,同時分析了mbd3基因誘導表達的組織特異性,劑效和時效關系;結合mbd3基因啟動子區序列分析,以- 164 ? - 179bp雙鏈dna序列合成探針,經[ - ~ ( 32 ) p ] dctp標記后通過電泳遷移率改變實驗( emsa )和south ? westernblot方法對參與mbd3在肝臟中誘導表達調節的轉錄因子進行分析。
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