次代培養細胞 的英文怎麼說

中文拼音 [cìdàipéiyǎngxìbāo]
次代培養細胞 英文
subculturing cell
  • 次 : Ⅰ名詞1 （次序; 等第） order; sequence 2 [書面語] （出外遠行時停留的處所） stopping place on a jou...
  • 代 : Ⅰ動詞1 （代替） take the place of; be in place of 2 （代理） act on behalf of; acting Ⅱ名詞1 （歷...
  • 培 : 動詞1. （在根基部分堆上土） bank up with earth; earth up 2. （有目的地使成長、壯大） cultivate; foster; train
  • 養 : Ⅰ動詞1 （供養） support; provide for 2 （飼養; 培植） raise; keep; grow 3 （生育） give birth to ...
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 胞 : Ⅰ名詞1 （胞衣） afterbirth2 （同一個國家或民族的人） fellow countryman; compatriot Ⅱ形容詞（同胞...
  • 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
  1. In trpsin tolerance assay. this virus could resist to 1 % trpsis at 37 in an hour. in acid tolerance assay, this virus was resistant to ph3. 0 and ph5. 0 at 37 in 2 hours, and the average infection litre of the virus decreased little. in heat assay, at 50, the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree. among these conditions, when at 50 in 30 minutes. the average infection litre of this virus decreased over 2 tilre. and when al 50 in an hour, cpe of ihis virus disappeared. when time was set for an hour. but with processed in different temperature as 50 60 70, 80, the virus losl the multiplication capacity complelely. in biological assay, we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory. then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second. with f81 cell line, after passaged for 12 times continuously with low concentration of fcs. the virus could produce cpe. however, with vero cell line. the virus could not procuce any cpe after many passages. the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig, fowl and cavy. by neutrolizaion assay, dxmv could be identified as a kind of ccv

    理化學研究表明，該病毒為rna病毒，對氯仿、乙醚敏感；胰酶試驗中，經37 、 1小時處理的病毒，仍然能夠在貓源細胞fcwf細胞上生長，並且毒力基本保持不變；耐酸性試驗中，病毒分別在ph5 . 0和ph3 . 0經37作用2小時，毒力僅下降一個滴度；耐熱性試驗中，該病毒在恆定溫度50 ，設定不同時間，從5分鐘到150分鐘，毒力均有不同程度下降，其中， 50作用30分鐘，病毒平均滴度下降2個單位； 50 ， 60分鐘， cpe消失；恆定時間1小時，設定不同溫度（ 50 - 60 - 70 - 80 ） ，病毒在細胞上完全喪失增殖能力， cpe消失。生物學試驗，利用實驗室現有條件，選擇不同的細胞系對該病毒進行培養，發現該病毒對貓源細胞fcwf最敏感； mdck細胞次之； f81細胞經多次傳代，亦可出現cpe ；而vero細胞則不敏感。血凝試驗表明，該病毒對豬、雞、人及豚鼠的紅細胞均無血凝性。
  2. In this report, we mainly covered the following aspects of " tissue organ regeneration and replication in situ " : 1 ) procedures of tissue organd regeneration and replication and replication in clnical practice ; 2 ) the discover and existence of potentiald regenerative cell ( prc ) ; 3 ) the proliferation, differentiation and regeneration law of potential law of potential regenerative cells ; 4 ) study procedure on tissue organ regeneration and replication from prcs in vitro based on the model of full skin organ regeneration in situ after extensive in vitro, set up the method and technology of searching life regenerative substance required in tissue organ regeneration and replication in situ. in this study, first, the whole human body is divided into 206 function units, which are the " tissue organ " in regeneration study. then the histology foundation of tissue organ regeneration and replication in situ is set up. in ordre to prove the existence of the potential regenerative cells and their potential baility and function, we established clinical tracking rechnique of skin organ regeneration in situ ; meanwhile, several tissue organ regeneration and replication in vitro models which represent different kinds of runctions were sucessfully set up, with all these techniques and models, we confirmed : 1 ) the existence, function and ability of pptemtoa regenerative cells ; 2 ) the importance of life regenerative substance ; 3 ) the feasibility of tissue organ regeneration and replication in situ ; 4 ) the big value of tissue organ regeneration and replication in situ in life science and medicine progerss. we also showed the possible foreground of capture cancer with this method and technologh. in this report, nearly 200 photographs of several tissue organ regeneration and replication in situ or in vitro demonstrated the whole process of tissue organ and big organ entities regeneration and replication from cells. the results of tissue organ regeneration and replication in situ mainly include : 1 ) whole skin organ regeneration and replication in situ ; 2 ) gastrointestinal mucosa tissue organ regeneration in vitro ; 3 ) hair follicle tissue organ regeneration in situ or in vitro ; 4 ) never tissue organ regeneration in situ ; 5 ) pancreas tissue organ regeneration and replication in vitro ; 5 ) marrow tissue regeneration in vitro ; 6 ) renal glomerulus and tubule tissue organ tugeneraation in vitro ; 7 ) heart muscle regeneration in vitro, etcl. in order to let more and more people know and understand this technology of tissue organd regeneration and replication in situ, herein, for the first time, we publicize the key points of actualizing this technology. also, we publicized the technology procedures and the frame constitute of life substances. we bilieve this is a big contribution to human science

    本研究報告，重點報道了組織器官的原位再生復制的臨床程序，報道了組織潛能再生細胞的發現和存在，以及該細胞的增殖分化和形成組織器官的變化規律.以燒傷后皮膚組織器官的原位再生復制為模型，研究出了體外組織潛能再生細胞復制組織器官的培養方法;以體外組織器官的復制為模型，建立了尋找原位組織器官再生復制所需生命物質的方法和技術.本研究，首先按人體的器官功能，分解為206個功能單位，確立了所復制的人體器官中的組織功能單位為組織器官，從而建立了原位組織器官再生復制的組織學基礎.為了驗證組織潛能再生細胞的再生潛能，建立了皮膚器官原位再生的實體臨床跟蹤技術，同時又建立了能代表有關器官功能類別的代表組織器官的原位和體外復制模型，以多組織器官的成功復制確定潛能再生細胞的作用，確定生命研究再生物質的重要性，確定組織器官原位再生復制的可行性，確定了組織器官原位再生復制的生命科學研究和醫學進步的重大應用價值，同時展示了用此方法和技術攻克癌癥的前景.本研究報告，以近二百幅多個組織器官原位和體外再生復制的實體圖片，展示了潛能再生細胞復制的組織器官和大器官司實體;展示了細胞再生復制器官的全過程.真實的報告了組織器官原位再生復制的成果.所公布的主要成果為:皮膚器官的原位再生復制;胃腸黏膜組織器官的原位和體外再生復制;毛囊組織器官的原位和體外再生復制;神經組織器官的原位復制;胰腺組織器官的體外復制;骨髓組織的體外復制;腎小球小管組織器官的體外復制;心肌的體外復制等.為了讓更多的人學會和掌握組織器官原位再生復制技術，本報告首次公布實施技術的重要環節和技術流程;首次公布了生命再生物質的框架和組成.作者自費研究成果對人類生命科學的一大貢獻
  3. The primary primordial germ cells is obtain from the human embryos after 4 - 8 weeks of pregnancy. after mechanical desection and enzymic digestion, the cells were cultured on mouse embryonic flbroblast or human embryonic flbroblast feeder layer inactivated by mitomycin. the medium contains several cytokines : lif ( leukemia inhibitory factor ), bfgf and foskolin

    從4 ? 8周的流產胎兒的原始生殖嵴部位分離到原始生殖細胞，經過機械和化學方法的解離后培養於事先用絲裂黴素處理過的小鼠胚胎成纖維細胞或人胚胎成纖維細胞飼養層上，培養基中添加了lif ， bfgef ， foskolin等細胞因子，此後大約每7天左右傳代一次。
  4. Bovine viral diarrhea virus were propagated by mdbk cells. after five to six continuously and rapidly passing, the cells emerged evident cytopathic effect. then the infected cells and its solution were harvested

    Bvdvnadl株屬于致細胞病變型毒株，用mdbk細胞培養增殖bvdv ，經5 - 6次連續傳代至出現明顯的細胞病變。
  5. 3 in the light and dark, the contents of soluble protein, the activities of peroxidase ( pod ), superoxide dismutase ( sod ) and esterase ( est ) were studied, and they all showed their changes respectively. 4 the alkaloids were accumulated primarily in the stationary phase

    4 、培養細胞中次生代謝物質的積累：光照和黑暗條件下生物堿的含量變化不盡相同，但第12d均出現一個峰值，並且從第18d起生物堿的含量呈上升趨勢。
  6. The results showed that : when cultured in the medium of m199, supplemented with 20 % bovine serum containing a moderate amount of antibiotics, the incubtion ph 7. 2 - 7. 4, the culture temperature 28. the primary culture cells formed the dense single wall within three weeks, the generation time of the subculture cells was 5 - 6 days, most cultured cells were fibroblastic - like cells with few epithelial - like cells

    研究初步表明：在m199培養基加入20小牛血清（常規量雙抗） ， ph在7 . 2 - 7 . 4之間， 28的培養條件，四倍體鯽鯉魚腎組織原代培養三周左右即可形成緻密單層，傳代細胞為5 - 6天左右傳一次。培養細胞以成纖維樣細胞為主，有少數上皮樣細胞。
  7. 2. the plasimd encoding human carcinoembryonic antigen was transfected with lipofectine into human dcs and human tumor cells such as mgc803, ls174t and eel09. the culture supernatants were collected for assaying carcinoembryonic antigen by. 3

    1640培養液中，在37ac ， 5 co ，的培養箱內培養， 』每3心傳代一次，取對數生長期的細胞進行實驗。
  8. The studies on cell immobilization culture of ginkgo biloba l, as well as the production of their secondary metabolite - ginkgolides were reported in this paper

    本文對銀杏細胞固定化培養及其次生代謝產物銀杏內酯的產生進行了較為系統的研究。
  9. Research advances on synthesis of secondary metabolities by plant cell culture

    植物細胞培養技術合成次生代謝物質研究進展
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