次培養 的英文怎麼說

中文拼音 [cìpéiyǎng]
次培養 英文
subculture
  • 次 : Ⅰ名詞1 （次序; 等第） order; sequence 2 [書面語] （出外遠行時停留的處所） stopping place on a jou...
  • 培 : 動詞1. （在根基部分堆上土） bank up with earth; earth up 2. （有目的地使成長、壯大） cultivate; foster; train
  • 養 : Ⅰ動詞1 （供養） support; provide for 2 （飼養; 培植） raise; keep; grow 3 （生育） give birth to ...
  1. A biweekly publication produced by k - 12 students as a resource to other k - 12 students

    -培養大專層次的幼教和小教音樂美術師資的師范學校。
  2. The primary results showed : using m199 as diluents containing 20 % bovine serum, it is better to freeze the cells slowly freezing at fist then increase freezing speed ( for example, from 0 to - 6 freezing speed is about - 0. 05 a minute, from - 6 to - 40, freezing speed is about - 0. 5 a minute ), studies on effect of various concentration of dmso demonstrate that about 12. 5 % dmso gave the highest post - thaw percentage of viable cells. the concentration of bovine serum had no different effect on the percentage of the viable embryo cells of misgurnus auguillicaudatus. the embryo cells derived 6 from the later stage of blastula offish is more resistant to the cryogen than the cells of early stage of blastula. the cells preserved in liquid nitrogen at - 196 were thawed and cultivated, a few cells were found adhere to the surface of culture vessel when the percentage of viable cell was more than 30 %. the cells in only two culture vessels were found to proliferated and gave rise to many small morphologically undifferentiated cells

    研究初步表明:以細胞培養液m199 （含2既的小牛血清，常規量雙抗）為凍存稀釋液對泥鰍胚胎細胞冷凍保存宜採取先慢后快的方式（例如，從0一一6 ，凍存速度為一0 . 05 / min ，再以一0 . 5 / min的速度從一6一一40 ） ; dmso的保護效應濃度為12 . 506左右;小牛血清的濃度對泥鰍胚胎細胞的成活率影響不明顯;囊胚晚期細胞抗凍性比中早期強;通過對不同批次的凍存細胞解凍培養，解凍后成活率為30 %以上細胞培養數天後均有少數細胞貼壁，但只發現兩瓶培養細胞有明顯增殖現象產生許多未分化的小細胞。
  3. In trpsin tolerance assay. this virus could resist to 1 % trpsis at 37 in an hour. in acid tolerance assay, this virus was resistant to ph3. 0 and ph5. 0 at 37 in 2 hours, and the average infection litre of the virus decreased little. in heat assay, at 50, the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree. among these conditions, when at 50 in 30 minutes. the average infection litre of this virus decreased over 2 tilre. and when al 50 in an hour, cpe of ihis virus disappeared. when time was set for an hour. but with processed in different temperature as 50 60 70, 80, the virus losl the multiplication capacity complelely. in biological assay, we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory. then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second. with f81 cell line, after passaged for 12 times continuously with low concentration of fcs. the virus could produce cpe. however, with vero cell line. the virus could not procuce any cpe after many passages. the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig, fowl and cavy. by neutrolizaion assay, dxmv could be identified as a kind of ccv

    理化學研究表明，該病毒為rna病毒，對氯仿、乙醚敏感；胰酶試驗中，經37 、 1小時處理的病毒，仍然能夠在貓源細胞fcwf細胞上生長，並且毒力基本保持不變；耐酸性試驗中，病毒分別在ph5 . 0和ph3 . 0經37作用2小時，毒力僅下降一個滴度；耐熱性試驗中，該病毒在恆定溫度50 ，設定不同時間，從5分鐘到150分鐘，毒力均有不同程度下降，其中， 50作用30分鐘，病毒平均滴度下降2個單位； 50 ， 60分鐘， cpe消失；恆定時間1小時，設定不同溫度（ 50 - 60 - 70 - 80 ） ，病毒在細胞上完全喪失增殖能力， cpe消失。生物學試驗，利用實驗室現有條件，選擇不同的細胞系對該病毒進行培養，發現該病毒對貓源細胞fcwf最敏感； mdck細胞次之； f81細胞經多次傳代，亦可出現cpe ；而vero細胞則不敏感。血凝試驗表明，該病毒對豬、雞、人及豚鼠的紅細胞均無血凝性。
  4. The double - base teaching is one of traditional features in the mathematics " education in our country, which focuses on how to cultivate the students " inquiry ability in the daily teaching. first of all, from the beginning of the double - base teaching, we pay attention to the original knowledge during the teaching of concepts, definitions, theorems and formulas. in the case of exercising lessons, we conclude, summarize and train the student ' s emanative ideations

    重視雙基教學是我國中學數學教學的傳統特色，在日常教學中培養學生的探究能力，應首先從雙基教學開始：在概念、定義、定理、公式的教學中注重知識的起源；在例習題教學中，注重歸納、小結及訓練學生的發散思維能力；在復習課的教學中注重各知識的相互聯系；其次，由於數學開放題具有良好的探究功能，因此，在日常教學中適當創設數學開放題，也是培養學生探究能力的一個重要途徑。
  5. Standard specification for disposable glass culture tubes

    一次性生物培養玻璃試管的標準規范
  6. The plan of the research institute becomes, in the near future, with the ocean chemistry, the development and engineering turn of halo bios resources, the ocean information technique processing, ocean environment and ecosystems protection, etc. aspect for center of research direction, and gradually formats the aspect advantages in using of the salt water and the half salt water ( the haloid water ) chemistry resources, the ecosystem system monitor and controlling, algae cultivating and biology active material, the development research of nourishment supplies and bio - products, the ocean environmental protection and ecology etc., end can become one of important bases in the ocean realm of nation and tianjin for high level creative talented person development, hi - technique research and result conversion, high decision advisory, and the double that attains the economic performance and social performances win

    研究所計劃在不久的將來形成以海洋化學、海洋生物資源開發及工程化、海洋信息技術處理、海洋環境與生態保護等方面為中心的研究方向，並且逐步在鹹水及半鹹水（鹵水）化學資源利用，生態系統監測與調控；藻類培養及生物活性物質、營養物資及生物製品開發研究；灘塗資源開發利用；海洋環境保護和生態學等方面形成優勢，最終可以成為國家和天津市海洋領域高水平創新人才培養、高新技術研究和成果轉化、高層次決策咨詢的重要基地之一，達到經濟效益與社會效益的雙贏！
  7. After entry of wto, there are still many maladjustments in guangxi ' s seed industry, such as the weak foundation of sees industry development ; the unformed market of the seed industry for fair competition ; small - scale seed enterprises ; no systematic connection among the cultivation, breeding and marketing ; lower qualification of staff who work in the seed industry and with weak sense of laws, and lack of the knowledge in operating experiences in the international market and trade etc. yet the un - efficiency system, unclear property right in enterprises, the lack of an effective mechanism to promote the rational use of resources in the seed industry and the lack of such concept as " the government creates environment and enterprises create fortune " are the deep - seated causes of the problems in guangxi ' s seed industry. therefore, the key points for promoting development of guangxi ' s seed industry under the wto framework are to focus on the promotion of the developing capability of seeds " integrated products, constantly deepen reforms, to adjust various relevant factors in the system of the seed industry which is inconsistent with each other, and to establish a new - pattern system with evident characteristics of the time spirit in order to meet the requirements of the market economy. hereinto, the specific strategies and measures for promotion of guangxi ' s seed industry development under the wto framework include kee ping up reform and innovation of the system of the seed industry, executing of non - nationalization reform in state - owned seed enterprises, formulating and executing relevant supporting policies, the improving the legal system in the seed industry, increasing public financial support on the seed industry, promoting the integration of cultivation, breeding and marketing, strengthening human resource development, developing the main body of the seed industry ' s market and making proper conditions for the functions of seed associations in the seed industry development etc

    研究結果認為：發展種子產業應該把著眼點放在促進種子整體產品的開發上；種子產業的發展依賴于能充分發揮整體功能的新型種業體系的構建，而目前廣西種業體系中的品種選育、種子生產加工、種子經營以及政府管理、公共支持和社會服務六個主要組成部分都存在明顯的缺陷與不足；廣西種子企業綜合競爭力總體處于較弱水平；面對wto ，廣西種子產業仍有諸多的不適應，突出表現在產業發展基礎薄弱、尚未形成可以公平競爭的種業市場、種子企業規模小、育繁銷脫節、種業人才素質不高、種子企業法律意識淡薄、國際市場運作經驗和國際貿易知識不足等多個方面，而體制不順、企業產權不明晰、缺乏促進種業資源合理流動的有效機制以及「政府創造環境，企業創造財富」的正確理念正是導致目前廣西種子產業不能適應入世需要的深層次原因；因此，以提升種子整體產品開發能力為核心，不斷深化改革，調整種業體系中不相協調的各有關因素，構建起符合市場經濟體制要求的具有鮮明時代特徵的新型種業體系，是wto框架下加快廣西種子產業發展必須堅持的指導思想；其中，加快種業體制改革和創新、實施對國有種子企業的非國有化改造、制訂落實有關扶持政策、完善種子法律法規體系、加大公共財政對種子產業的支持力度、推進育繁銷一體化的形成、加強人才培養與引進、壯大種業市場主體、實行重點突破戰略、發揮種子行業協會作用等等，都是wto框架下加快廣西種子產業發展應該採取的具體對策措施。
  8. Abstract : the early embryo developmental block is a common phenomenon in mammal when embryos are cultured in vitro. many studies of phosphorus, glucose, hypoxanthine and cytoplasmic factors on early embryo developmental block carried out by different methods such as morphology, biochemistry, molecular biology and micromanipulation have been reviewed. the merit and shortcoming were analyzed and the necessity of using simple or components limited media overcoming early embryo developmental block were also reviewed. media that have been shown effective in overcoming early embryo developmental block in mouse, rat, hamster, rabbit, pig, sheep, cattle and monkey were listed

    摘要哺乳動物胚胎在體外培養中普遍存在早期發育阻滯的現象.對此，人們用形態學、生物化學、分子生物學、顯微操作等手段開展了磷酸、葡萄糖、次黃嘌呤和細胞質因素對早期胚胎發育阻滯的影響的研究.本文綜合分析了共培養系統的優缺點.說明了採用完全成分已知的培養液對進行有關研究的必要性.列出了有效運用於克服小鼠、大鼠、倉鼠、兔、豬、羊、牛、猴等動物早期胚胎阻滯的成分已知的培養液的名稱。
  9. Rat cumulus - enclosed oocyte ( ceo ) and denuded oocyte ( do ) both spontaneously resume meiotic maturation in vitro, when cultured for 2h, the germinal vesical breakdown percentage of do reaches up to 88 %, and ceos get 53 %. oocytes begin to extrude first polar body 1 ( fb1 ) when cultured for 8h. mouse oocyte spontaneous maturation in vitro can be inhibited by 4mm hypoxanthine ( hx ) or 25um 3 - isobutyl - methylxanthine ( ibmx ). while, 4mm hx can not inhibit the spontaneous maturation of rat oocyte, and the lowest effective concentration of ibmx to inhibit rat oocyle spontaneous maturation in vitro is 50um. treated with the same concentration of ibmx, gvbd percentage of rat ceo is lower than that of do, the results indicat that cumulus cells delay rat oocyte spontaneous maturation and potentate the inhibition effect of ibmx, but it ' s not the case on mouse

    Ceo和do培養8h時都開始排出pb1 ；在卵母細胞成熟抑制模型中， 4mm次黃嘌呤（ hx ）和25 m的異丁基甲基黃嘌呤（ ibmx ）是小鼠卵母細胞成熟的有效抑制劑，但4mm的hx抑制大鼠卵母細胞成熟效果不理想，用hx - m199和hx - mem培養24h ， gvbd率分別為61 ， 81 ，而ibmx有效抑制大鼠卵母細胞自發成熟的最小濃度為50 m ， ibmx可以降低大鼠ceo和do的gvbd率，但ceo中的卵母細胞gvbd率要顯著低於do的gvbd率， 100 m的jbmx處理24h后，大鼠ceo和do的gvbd率分別為24 ， 84 ，提示卵丘細胞的存在可以延遲大鼠卵母細胞的自發成熟，有助於抑制卵母細胞成熟，但在小鼠上ceo和do的自發成熟差異不明顯。
  10. Koch perceived that it would be far better if one could solidify a well-tried liquid medium with some clear substance.

    郭霍設想，如果能用某種透明物質將經過多次試驗的培養液固化可能更好。
  11. In 2000, dr. jing also worked as the visiting professor in kumamoto university, japan for three months

    已指導培養博士生3名、碩士生2名，其中4人次獲中科院上海生化所所長獎學金。
  12. The results showed that lamellae were fit tissue for mycelia isolation, and success percentages of mycelia isolation with media pdas, pdaw, bm, pda are 74. 4 %, 35. 5 %, 15. 6 % and 8. 9 % respectively. the results also showed that stipe, mycorrhizae, and soil with the fungi are not fit for tricholoma matsutake isolation

    另外，馬鈴薯葡萄糖麥數濾汁培養基（ pdaw ） 、 bm培養基、 pda培養基也從菌褶2曾東方博士論文:腐生與共生食用菌菌絲體分離、培養及其dna鑒定研究分離到松茸菌絲體，分離成功率依次是35 . 5 % 、巧
  13. The results of the experiments showed peptone was the best nitrogen source in solid culturate, the second was yeast extract

    結果表明；在固體培養中，病菌對氮源的利用以蛋白腖為最好，其次為酵母膏。
  14. Petri dishes - specification for plastics petri dishes for single use

    陪替氏培養皿.第2部分:一次使用的陪替氏塑料培養皿規范
  15. Phaeocystis globosa ( hong kong ) was examined under laboratory conditions with batch culture method. the growth cycle of phaeocystis globosa takes about 20 ~ 30 days in batch culture

    在實驗室對球形棕囊藻（香港株）進行了純種培養，在批次培養中，球形棕囊藻的生活周期約為20 30天。
  16. This is the first time i cultivated narcissus and is only thing placed to celebrate the spring festival at home

    這是我第一次培養水仙花?也是春節唯一的應節品。
  17. The results showed that : when cultured in the medium of m199, supplemented with 20 % bovine serum containing a moderate amount of antibiotics, the incubtion ph 7. 2 - 7. 4, the culture temperature 28. the primary culture cells formed the dense single wall within three weeks, the generation time of the subculture cells was 5 - 6 days, most cultured cells were fibroblastic - like cells with few epithelial - like cells

    研究初步表明：在m199培養基加入20小牛血清（常規量雙抗） ， ph在7 . 2 - 7 . 4之間， 28的培養條件，四倍體鯽鯉魚腎組織原代培養三周左右即可形成緻密單層，傳代細胞為5 - 6天左右傳一次。培養細胞以成纖維樣細胞為主，有少數上皮樣細胞。
  18. Nowadays, the education of technical talents in our country mainly consists of higher training schools and higher vocational schools. while vocational and technical education at the graduate level is still at a trial stage. for this reason, this research is aimed at the analysis and exploration of cooperative education of technical talents at the graduate level between colleges and enterprises

    現今我國高級技術型人才以高等專科學校和高等職業技術學校的大專層次培養為主，至於本科層次職業技術教育處于試辦階段，為此本文研究旨在對本科層次技術型人才的產學合作的培養模式作一分析探索。
  19. Professional technical education must be a kind of education that offers a variety of choices in educational levels, training goal, main body of running a school and educational forms to meets variety of demands for study and development

    摘要職業教育必須是一種在教育層次、培養目標、辦學主體、教育形式等方面提供多種選擇，滿足多種學習和發展需要的教育。
  20. Education for pre - determined target : namely high learning institutions combine the undergraduates ' academic standard with the rural school situation, conduct reform in the curriculum of teacher education, lay a stable foundation of three kinds of knowledge for normal college students, that is, extensive scientific and literary knowledge, systematic knowledge of professional development and educational sciences, and intensive training of basic teaching skills, which will enable the students to be compatible to teaching task after their graduation

    定標培養：學校嚴格按照普通高等學校本科層次培養規格及標準，並結合農村山區基礎教育具體情況，大膽進行師范類課程改革，拓展師范生三類知識，即廣博的科學文化知識、系統的學科專業知識和堅實的教育專業知識，並著力強化訓練教育教學基本技能，使畢業生走上崗位就能完全勝任教育教學。
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