次基因組的 的英文怎麼說
中文拼音 [cìjīyīnzǔde]
次基因組的
英文
subgenomic-
It was firstly reported in china. the third part rthe capsid protein vp | gene was expressed. 810 bp fragment was inserted into vector pgex - 4tl, and then transformed into e. coli bl21
這是國內首次對aev基因組全序列的分析;第三部分: aev外殼蛋白vp _ 1基因在大腸桿菌中的融合表達、純化及活性研究。For functional genomics, huge est databases from multiple tissues of a number of tree species have been rapidly accumulated, and molecular analyses on secondary growth and wood formation, flowering, and cold hardiness have given some insights into the metabolic pathways of those tree - specific development processes
功能基因組學方面,已分析了主要造林樹種多種組織的轉錄組est序列,對林木次生生長與木材形成、開花和抗寒性的形成等過程開展了功能基因組學研究。Some of these events may appear to recapitulate features of primitive genomes, providing indirect clues as to how early genetic systems could have functioned
隔代遺傳中的一些事件可能會再次反映出原始基因組的特徵,從而為早期遺傳學系統是如何擁有功能提供間接的線索。First, three isozymic systems ( lactate dehydrogenase ( ldh ), esteraes ( est ), malate dehydrogenase ( mdh ) ) extracted from threepopulations of mandarinfishes were detected by discontinuous vertical plate polyacrylamide gel electrophoresis ( page ). several loci were tested but none polymorphic locus was detected in qiupu river population. the results showed that : ldh can be used as biochemical markers to identify these three populations of mandarinfishes
首先,本試驗採用聚丙烯酰胺凝膠電泳( page )對我省三個水域鱖魚群體3種同工酶( ldh 、 est 、 mdh )的不同基因座位進行了檢測,結果表明: ldh同工酶可以作為區分秋浦河鱖魚、長江鱖魚兩個群體與萬佛湖鱖魚群體的生化遺傳標記;其次,運用rapd技術分析了三群體鱖魚的基因組dna的多態性。In this work, some species of palmae cultivated in the xiamen botanical garden had been selected to be analyzed their genetic diversity with rapd ( random amplified polymorphic dna ) technique. according to the result of genomic dna amplified with pcr ( polymerase chain reaction ), genetic distance and similarity between different palm species were calculated on nei " s estimate of similarity and genetic distance. a primary but first time research at the phylogenetic relationships of some genera and species, the molecular classification and identification of some puzzling species of palmae was carried out through upgma ( unweighted pair group mean average ) cluster analysis of the genetic distance together with comparative study of the morphological structure characteristics
本文在初步調查分析了棕櫚科植物在我國的自然分佈、引種馴化情況以及該類植物在廈門地區栽培應用狀況的基礎上,首次採用rapd分子標記技術,對廈門萬石植物園引種的一些棕櫚科植物的遺傳多樣性進行了研究,根據pcr對基因組dna擴增的結果,用nei ' s相似性系數計算了不同植物間的遺傳距離和遺傳一致度,通過對遺傳距離的upgma聚類分析,並結合形態分類的特點,對棕櫚科植物的屬、種間的系統分類關系和一些疑難種的分類鑒定進行了初步研究。Which shared 10 % ~ 73 % identities to other rips from plants and 37 % ~ 73 % to other rjps from cucurbitaceae. six novel rip gene fragments ( 408 ' bp ), 1 from benincasa hispida and 5 from cucurbit a moschaia
根據葫蘆科rip上、下游兩段高度保守的氨基酸序列設計簡並引物對ly1 ly2 ,對基因組dna進行pcr擴增,首次建立了rip新基因快速篩選體系。In order to provide the scientific basis for the good agricultural practice ( gap ) of sichuan bai zhi, rapd was firstly used to analyze the genetic polymorphism of different type from sichuan bai zhi and hangzhou bai zhi. the result showed that different color of petiole has not obvious relationship to whether to bolt or not and different localities have more influences on genetic diversity than different color of petiole
為配合川白芷生產質量管理規范化( gap )研究,本文首次採用rapd技術對川白芷生長過程中不同形態的四個類群及杭白芷進行基因組dna多態性分析,認為川白芷葉柄顏色的不同與是否抽薹無明顯的相關性,而產地的差異對遺傳變異的影響大於因其葉柄顏色不同所造成的影響。When both genes were co - expressed in e. coli, the activity of ppsa varied from 2. 1 - 9. 1 fold comparing to control, but the activity of tkta was relatively stable ( 3. 9 - 4. 5 fold ). whatever the two genes were expressed respectively or cooperatively, both could promote the production of dahp, the first intermediate of the common aromatic pathway, but co - expression was more effective on forming dahp and screened ppt - and ptp - as more effective. the results demonstrate that co - expression of ppsa and tkta can improve the production of dahp, and what ' s more, when multigenes co - expressed, the recombinant which has coordinated enzymes activity is optimum
莽草酸途徑的最優化和整體調控基因csra的敲除正是上述改變的分子基礎,同時也為三種芳香族氨基酸的基因工程菌的構建打下了基礎; 7 .在國內外首次實現了共同途徑限制性底物關鍵酶ppsa刁無『及arog與分支途徑關鍵酶基因phea的串聯高效表達,所構建的重組質粒ptga ,其ppsa 、 tkta 、 arog 、 cm和pd的酶活分別比對照提高了3 、 2 、 2 , 5 、 4 、 2 . 3倍,且其酶活比較協調一致; 8 .將ptga導入到篩選的基因敲除和基因替換菌株大腸桿菌31884 c甲b中,搖瓶發酵證實比以往所構建的基因工程菌株具有較高的phe產量和糖轉化率率,分別為0 . 448 %和22 . 4 % 。It is very important to investigate and clone related genes under fe - deficiency stress in malus xiaojinensis, which not only provides the probability for exploring molecular mechanism of the absorbability and transport of fe, but also may enhance the resistance under fe - deficiency stress of plants by inserting related genes into chromosome genome
因而研究和克隆缺鐵脅迫相關基因不僅為進一步研究蘋果吸收、轉運鐵的分子機制提供基礎。其次,在提高植物對鐵脅迫抗性的遺傳育種中,可以利用基因工程技術,將鐵脅迫相關基因整合到作物染色體基因組中,從而達到有效控制鐵營養的目的。The biologic toxins produced by bacteria and virus have important effects to organic metabolism and reproduction. the study on bacterial toxin at molecular level, especially, on complete nucleotide sequence determination of pathogenic micro - organism has make it possible to comprehend pathogenic micro - organism pathogenesis and its rule. recently complete nucleotide sequences of near ten bacteria have been examined
細菌、病毒等所產生的生物性毒物對機體的代謝、繁殖機能有著重要的影響,目前對細菌毒素的研究比較透徹,已經上升到分子水平,特別是通過病原微生物全基因組序列的測定,使人們從更高層次上把握病原微生物的致病機理及其規律成為可能。A sds - iso - propanol method suitable for tea plant, which was plentiful of tea polyphenols, had been developed using a modification of chen darning ' s method from different sample storage conditions such as fresh, dry and frozen shoots. it was a quick, easy, economical and effective method. the tactics were as follows : before the cell nuclear membranes were decomposed, the tea polyphenols and proteins etc. were removed
該法提取緩沖液使細胞維持一定的滲透壓,研磨時使細胞核基本保持完整;在細胞核被裂解之前去除細胞質中的茶多酚、大部分蛋白質和rna ;而後用sds裂解細胞核,異丙醇或乙醇沉澱dna ,這樣能經濟、快速和有效地從富含茶多酚、茶多糖等次生物質的茶樹新梢中提取基因組dna 。Finally, a tranfer vector named as pltk - ha was constructed based on pltk - uni with insertion of ha gene of h3 subtype srv. the pltk - ha and prv bartha - k61 genomic dna were co - transfected into 50 - 70 % confluent vero cells in 6 - cm dishes. based on the expression of lacz gene, recombinant prv were selected and purified by blue - colored virus plaque
利用脂質體介導的方法,將pltk - ha與prvbartha - k61基因組共轉染于亞單層vero細胞,依據報告基因lacz在細胞中的表達,篩選藍色蝕斑的重組病毒,經數次蝕斑純化后, pcr鑒定、 western - blot分析。4. the improved method of ctab isolation of total dna is suitable for extraction of total dna from insects of troides
首次採用改進的ctab法提取裳鳳蝶基因組dna ,所提樣品完全可以滿足rapd及線粒體dna的擴增。Location of the hbrp gene in the chromosome according to stanford g3 rh panel, a pair of primers are designed in the 3 " - utr region of the gene which is to be localized and human genome dna is amplified in advance whose products are examined by electrophoresis. then g3 panel is amplified and this process is repeated twice. the pcr result is sent to stanford human genome center ( shgc ) http : / / www - shgc
Hbrp基因的染色體定位使用stanfordg3rhkadiationhybrid )嵌板,在所要定位基因的3 』非翻譯區( untranslatedregion , utr )設計引物,預先擴增人基因組dna ,電泳檢測產物大小,繼之擴增g3rhpanel ,重復兩次,將pcr結果輸人斯坦福人類基因組中心( shgc )的主頁( http : 、 shgcThis hypothesis provide a new thinking on the action of steroid hormone on neurons, and is both a challenge and a supplement to the traditional genomic theory, which held that the action of steroid hormone is solely mediated by its intracellular cytosolic receptor within the cell nucleus
年代首次在國際上提出糖皮質激素作用於神經元的快速、非基因組機制或膜受體假說。這是對傳統崽體激素基因機制或細胞學說的挑戰與補充,受到國際學術界的高度評價,曾應邀在第Based on new materials and methods, this paper analyses the tandem and segmental duplication, then estimates the num of the lost gene after gene duplication in the arabidopsis gene family, and we find an evidence for the whole genome duplication in arabidopsis which happened about 80 million years ago
採用新的數據和方法研究擬南芥基因組的基因倍增過程,通過分析串聯基因倍增和大規模基因倍增的存在比例和同義置換率分佈,並估計大規模倍增后基因流失的比例,揭示了擬南芥基因組一次非常明顯的全基因組倍增,採用科學的方法佑計這次倍增發生在約8000萬年前。New minimally heated refrigerated foods are raising concerns about the hazard, he will tell delegates. other topics include how the complete genetic blueprints or genomes of food - poisoning bacteria such as campylobacter are transforming the science of food safety, and the latest research into potentially deadly strains of the e. coli gut bacterium
本次研討會的其它議題還包括:諸如彎曲桿菌這類食源性有毒細菌的完整基因圖譜或基因組圖譜是如何轉化成為食品安全科技的,以及對大腸桿菌這種腸內細菌有可能轉變成致命菌株的最新研究成果。With the appearing of transgenic animal, it is possible that people can rebuild the genome of animals by gene recombination and contrive research on relative gene ' s structure and function in viviperception. this is a multidimension system of gene research with regard to providing new approaches and thoughts from molecule to individual arrangement
轉基因動物的出現,使人類可以通過基因重組的各種方法人為的改造動物基因組,在動物活體水平上研究有關基因的結構和功能,是一個四維時空的研究體系,為從分子到個體的各個層次,多維的研究基因提供了新的方法和思路。This paper reports the results of studies on sequence analysis of helicoverpa armigera single - nucleocapsid nucleopolyhedrovirus ( hasnpv ) / / mdlll - l fragment, function anaysis of hasnpv two conserved genes, ha94 and hal32
本文首次對hasnpv的兩個桿狀病毒保守基因ha94和ha132的功能進行了研究,並報道了hasnpv基因組的hind - l片段的序列分析結果。Thl2 gene was finally amplified by pcr with template genomic dna and cdna from roots, leaves, petals, stigmas and ovaries during bud stage in brassica oleracea l. ( 200110197 ) and brassica napus l. ( y578 - 2 ). the dna sequencing result shows that the genomic gene of thl2 is approximately 900 bp from brassica oleracea l. in contrast to that of 850 bp from brassica napus l.
採用阿r和rtpcr技術,從甘藍和油菜基因組和柱頭總則a中擴增獲得了thlz基因,基因組中得到的基因大約為900hp和850hp , cdna大約為477hp 。在thlz基因中首次發現了兩個內含于,並且甘藍和油菜thlz基因的內含于序列同源性低於ich ,並且大小也有差異。分享友人