母基板 的英文怎麼說
中文拼音 [mǔjībǎn]
母基板
英文
mother board-
There was something contagious in kit's laugh, for his mother, who had look grave before, first subsided into a smile.
基特的笑好象有傳染性似的;因為他母親先前本是板著面孔,這會兒也泛起了笑容。Cloning and expression in e. coli of lactase. specific primers were designed according to the sequence of the beta - galactosidase gene from kluyveromyces lactis. klac gene was amplified by pcr and subsequently cloned
乳糖酶基因的克隆及原核表達以乳酸克魯維斯酵母( kluyveromyceslactis )菌株k538的基因組dna為模板,設計引物,利用pcr獲得乳糖酶基因klac ,經dna測序驗證,得到克隆t1549 。It is formed when an irritant gets inside of a shell and the shell protects itself by coating the irritant with the same material of its lining. in essence it is formed in the same way pearls are formed
在菲律賓,常見的珍珠母工藝品是茶杯墊,一般用從珍珠貝殼內側剝離出來的珍珠母貼在金屬基板上,然後用一條很窄的金屬薄片鑲邊。One dataset available for such an analysis involved gene expression profiling of the early growth factor response to platelet derived growth factor pdgf in a human glioblastoma cell line ; this study differentiated genes whose expression was regulated by signaling through the phosphoinositide - 3 - kinase pi3k versus the extracellular - signal regulated kinase pathways
本文採用人膠質母細胞瘤細胞對血小板衍生生長因子的早期反應特徵性基因表達數據,以區分通過磷酸肌醇- 3 -激酶和細胞外信號調控激酶信號途徑調控表達的基因The anchor bolts and nuts must be processed and used as a whole set. the embedded anchor bolts for equipment foundation shall be installed and fixed with positioning forms
地腳螺栓與螺母必須配套加工,配合使用。設備基礎的預埋地腳螺栓採用定位模板安裝固定。Firstly, the paper introduced the commonly hardware configure of microcomputer relaying and the function of every module of industry controlling computer std90386. secondly, the paper introduced several types busbarprotection which was commonly installed on electric power system and the characteristic of mv or lv system with low current grounding. based on it, the paper put forwards the theory of percentage - restraint differential relay. after that, in the discussing every problems of the relaying working the paper put the emphasis on the negative effect of the ct saturation and countermeasure
本文首先介紹了微機保護裝置的一般硬體配置以及作為裝置硬體平臺的std90386工控機各組件模板的功能,然後分析了目前電網系統裝設的母線保護的各種類型以及中、低壓小電流接地電網的特點,在此基礎上分析、介紹了作為本保護動作原理的「比率制動式差動保護」原理。In this article, the advanced structure of hybrid peptide mae is predicted with software. the fused gene mae - intein - cbd is amplified by pcr with the template of plasmid ptyb2, and then it is cloned into expression vector plasmid ppic9k. after verified by restriction enzyme analyzing and sequencing, the vector is transferred into the eukaryotic host ( yeast pichia
並以已構建的載體ptyb2 - mae為模板,通過pcr擴增出融合基因mae - imein - cbd ,將其克隆于表達質粒ppic9k中,通過鑒定並測序正確后,電轉化真核表達宿主? ?畢赤酵母菌株gs115 ,通過營養缺陷型培養基篩選重組子,再利用g418抗性篩選出整合有多拷貝外源基因的重組子。The research showed that their interactions in yeast two - hybrid system are still steady when the vector was exchanged, in contrast, the interaction was disappear when the reading frame of each positive had been changed. the four positives were subcloned into suicide plasmid so that gene mutant strains could be constructed via conjugation and homologous recombination
為了進一步驗證這些克隆的編碼產物與nifa蛋白的相互作用,將它們與nifa基因互換載體后共轉化酵母,仍然可以激活三個報告基因的表達,而陽性克隆移碼表達的重組質粒和pgbd - nifa的共轉化物則不能在選擇性平板上生長。Insulating materials based on mica. part 3 : specification for individual materials. sheet 9 : moulding micanite
雲母基絕緣材料規范.第3部分:專用材料規范.活頁9 :模製膠合雲母板Insulating materials based on mica - part 3 : specifications for individual materials - sheet 9 : moulding micanite
雲母基絕緣材料規范.第3部分:單項材料規范.第9活頁:模製膠合雲母板The gene encoding tannase was amplified from the total dna of aspergillus oryzae by pcr and was expressed in e. coli and pichia pastoris. the gene was insered in the expression plasmid pse380. the recombinant plasmid pse380 - tan was transformed into e. coli strains dh5a, top10, bl21 ( de3 )
本論文以米麴黴aspergillusoryzae的總dna為模板, pcr擴增了單寧酶基因( tan ) ,並分別嘗試了利用大腸桿菌( escherichiacoli )和巴斯德畢赤酵母( pichiapastoris )進行表達。Specification - machine tool components - levelling screws, nuts and seating plates
機床零件.第9部分:整平螺釘螺母及基座板The objective of this research is to transform the cloned phytase gene into pichia pastoris in order to obtain high - yield phytase - producing strain and to optimize the engineered yeast media recipes for the scale - up production of phytase. main results of this research are as follow : 1, xba i - linized recombinant plasmid ppic9k - phya was transformed into pichia pastoris by gene pulser. 98 positive transformants showing measurable phytase activities were screened on md plates and ypd plates containing g418. they all grew quickly on both md plates and mm plates, which proved their phenotypes of methanol utilization were mut +
主要實驗結查如下:西南農業大學碩士學位論文1 、用乃ai酶切攜帶植酸酶基因表達片段的重組質粒ppicgk夕句) a ,回收dna ,用genepulser電擊轉化畢赤酵母,塗布md平板,又用含不同濃度g418的ypd平板進行抗性篩選,得到98個可檢測到植酸酶活力的陽性轉化子,它們在md 、 mm平板上均表現快速生長,說明其甲醇利用表型是mut干。The mutant pel - d92l was expressed in pichia pastoris gs115, sds - page detection showed that the expression product pel - d92l - gs is different from pel - gs, and its " yield decreased dramatically, the themostability of pel - d92l - gs is also different from the pel - gs, but their optimum temperatures are same. 3. directed evolution of pel through random mutagenesis mutagenesis pcr carried out in error - prone conditions was used on the vector psk - pel, using the oligos " beginning " and " end ", homologous to the 5 ' and to the 3 " ends of the gene of pel respectively
三、 pel基因的隨機誘變用易錯pcr方法對pel基因進行隨機誘變, pcr產物與ppic3 . 5k連接,轉化大腸桿菌,獲得的混合質粒電轉化畢赤酵母gs115 , omm平板篩選適于低溫或對熱穩定的重組子,篩選獲得一株最活反應溫度、熱穩定性、發酵酶活均有提高的突變體pel - ep5 - gs ,其最活反應溫度為45 ,比野生型高出5 ; 40處理30min殘留活性為56 ,大大高於野生型的6 ;初始ph7 . 3528條件下培養72h ,發酵上清酶活為325u / ml 。The 1. 488kb dna fragment was sequenced and ligated to pbi121 vector and transformed into otsa deficient of e. coli strains ( ff4169 ). otsa gene is in charge of trehalose - 6 - phosphate synthesis in e. coli. in growth curve experiment, the transformants that carried the 1. 488kb dna fragment grew well in minimum medium, which contains 0. 5mol / l nacl, while control strains could n ' t endure it
提取釀酒酵母的總dna ,以此為模板,採用pcr的方法從釀酒酵母中克隆出了1 . 488kb的海藻糖合成酶基因tps1片段,通過xba和sma雙酶切,與同樣經過xba和sma雙酶切的puc118載體質粒連接,轉入大腸桿菌dh5中,通過藍白斑篩選重組子。Total rna was extracted from pituitary of new butchered female goat for fsh - a and b subunit genes. cdna were synthesized by rt - pcr reactions respectively and these cdna were used as templates in pcr amplifications. the pcr products were 360bp and 390bp which just were the same length of the predicted goat fsh - a and p subunit genes
本試驗是從新屠宰的母山羊腦垂體中提取總rna ,反轉錄獲得cdna ,以此cdna為模板, pcr擴增目的基因片段,分別獲得長為360bp的山羊fsh -亞基dna片段和長為390bp的山羊fsh -亞基dna片段,與預期的目的基因片段大小一致。分享友人