母細胞轉化 的英文怎麼說

中文拼音 [mǔxìbāozhuǎnhuà]
母細胞轉化 英文
blast cell transformation
  • 母 : Ⅰ名詞1 （母親） mother 2 （泛指女性長輩） one s elderly female relatives 3 （配套的兩件東西里的凹...
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 胞 : Ⅰ名詞1 （胞衣） afterbirth2 （同一個國家或民族的人） fellow countryman; compatriot Ⅱ形容詞（同胞...
  • 轉 : 轉構詞成分。
  • 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
  • 轉化 : 1. （轉變） change; transform 2. [化學] inversion; conversion
  1. Construction and expression of yeast engineering yaccine : s14 / gnsag " as transformed to yeast host straln x33 by means of electroporation after ppiczaa s, . aresag " as l ined by saci enzyme. the single fungus, as choose and dibble inocu1ating in we and am plate, the positive fungus was gro ' ing in rm but not in w, and was 6 inoculated in ypd which included zeocine 500ug / ml and 1000ug / ml. 5 transformers were ampl if ied by pcr, three is same with positive control

    選取單個菌落分別點種到刪平板和md平板，找出在回d上生長正常， w上生長緩慢或不生長的菌落，即陽性菌落，再以陽性菌落分別塗布zeocine含量500ug加， 1000ug砌l的ypd平板，以高濃度的抗生素篩選高拷貝的酵母工程菌，在含500ug ml高濃度抗生素平板上獲得了15個轉化子，取其中5個進行pcr擴增，有3個擴增產物與陽性對照相同，說明此酵母細胞中已含有s hbsag融合片段，其中之一命名為p
  2. Using the hprt gene as a positive control, our result suggested that both the testis tissue and the male embryos from which sry transcription can be detected failed to yield any positive results of xist. female embryos at the pronucleus stage and 2 - cell failed to produce any positive result of sry and xist too. while since the 4 - cells period, xist is constantly transcribed until blastocyst stages

    然後利用實驗一確定的pcr條件，以hprt為陽性對照，用巢式rt - pcr對小鼠早期胚胎進行xist基因的轉錄分析，結果發現，轉錄sry基因的睪丸組織以及雄性胚胎，從受精卵發育到囊胚的過程中，基本上不轉錄xist基因；不轉錄sry基因的雌性卵母細胞和雌性胚胎，從出現原核開始，到發育至2 -細胞期的過程中， xist基因一直不轉錄，但是，從4 -細胞期開始，一直到孵化前囊胚階段，雌性胚胎都轉錄xist基因。
  3. The cumulus expansion, cumulus cells dna fragmentation, oocyte meiotic maturation and degeneration were determined 44 h after incubation. snp inhibited cumulus expansion and cumulus cells dna fragmentation in a dose - dependent manner

    結果發現， snp可劑量依賴性地抑制卵丘細胞的擴展和dna片段化，同時抑制ceos中卵母細胞的減數分裂恢復，抑制m向m期轉變。
  4. According to above consideration, experiments was carried out as below : first, targeted gene - human serum albumin ( hsa ) gene was obtained via pcr technology. secondly, the hsa gene was liganded with a plasmidprla22 whose two arms carry dna sequences necessary for crossing with the human a - lactalbumin yac to form an integration vector prla - hsa, then the integration vector plasmid was co - transformated into the right yac - bearing yeast with another plasmid plrh33 which carrys a selective gene

    因此，本試驗首先擴增出整合在酵母基因組里的人血清白蛋白（ hsa ）基因作為目的基因，並將人血清白蛋白基因插入到一個含有人-乳白蛋白yac同源序列的重組型質粒載體，以構建整合型載體，再與另一個帶篩選基因的質粒共轉化入含人-乳白蛋白yac的酵母細胞體內。
  5. The transfermants with highest resistance to g418 ( 4 g / l in ypd ) were screened to study their expression level in 150 ml shaking flask. having been induced with methanol for 36 hours, the target enzyme could be examined in the supernatant by measuring amidolytic activity

    首次將大連蛇島賅蛇毒類凝血酶成熟基回克隆到表達載體ppicgk中，經電激轉化至畢氏酵母菌株gs15中，再經甲醇誘導，在150ml搖瓶畔1獲得細胞外分泌表達產物。
  6. Blast cell transformation

    母細胞轉化
  7. Multicopy integrants were screened with g418 from pichia pastoris which contains recombinant plasmid, and induced with methanol to secrete interesting peptide. the supernate of pichia pastoris culture was analysed by sds - page and western blotting. a reactive band, which the apparent molecular weight is 36kd, can be detected with sheep anti - hcmv polyclonal antibodies

    重組質粒轉化巴氏畢赤酵母， g418篩選出多拷貝插入的單克隆，甲醇誘導多拷貝插入的單克隆酵母細胞分泌目的蛋白，培養液上清經sds - page電泳分析，在蛋白質印跡中檢測到培養液上清有一表觀分子量為36kd ，能與羊抗hcmv多克隆抗體發生發應的條帶。
  8. In y2h, rapgap from c. elegans was used as bait to screen c. elegans cdna library. after - lth screen, 63 colonies were checked

    在酵母雙雜交系統中，將ppc97 rpg 」與ppc86七dna文庫共轉化入酵母菌y190細胞，經過一lth篩選后，有63個菌落擬為陽性菌落。
  9. In order to establish the genetic transformation system of saussurea medusa maxism by agrobacterium rhizogenes, some work were done. the main results were following : 1 establishment of regeneration systems two systems of regeneration from saussurea medusa maxim were established without cold treatment. the somatic embryos were induced from callus cultured in mise for 35 days. the shoots were induced from cotyledon after cultured in misc 20 days, and from leave which were cultured in misl. the experiment showed that the carbon and glycine in the medium could help to increase the regeneration rate to 95 %

    為篩選水母雪蓮代謝突變體和轉基因研究奠定了一定的基礎，陳亞瓊： m質粒介導水母雪蓮的遺傳轉化及毛狀根中決刪合成的調節p義摘要也為研究類黃酮代謝途徑的關鍵酶基因的轉化、高效表達及作用機理提供了理想的實驗體系。主要的實驗結果如下： 1水母雪蓮高頻再生體系的建立通過體細胞胚胎發生途徑和器官發生途徑，水母雪蓮（ squssureamedusamaxim ）可以在常溫下獲得再生植株。
  10. Furthermore, these cells had transformed themselves into the insulin - producing islet beta cells

    並且，母血細胞還自形轉化為可以生產胰島素的胰島細胞。
  11. In our work, different hbv pres gene fragments were amplified by pcr, cloned into pgbkt ? vector in yeast two - hybrid system 3, and then transformed ah 109 yeast cells. the results showed that these pres expression products were nontoxic to yeast cells, but all self - activated the yeast two - hybrid system

    本研究將hbv前s區不同片斷進行擴增，克隆入酵母雙雜交系統3中的pgbkt7載體，轉化酵母細胞ah109 ，經檢測，證實了hbv前s區不同片斷對酵母細胞無毒性作用，但是都存在自激活作用。
  12. In this text summarizes the research progress of isoflavones and which synthesized characters and transfered research in non - legumes including arabidopsis 、 tobacco 、 maize bms cells and yeast in the last years

    本文綜述了近幾年來大豆異黃酮的研究進展及其在非豆科植物擬南芥、煙草、玉米bms細胞和酵母的轉化研究及合成特點。
  13. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
  14. Multi - copies insertion transformants were screened on g418 plates. the recombinant protein was proved to have biological activity of hydrolyzing n - carbamoylphenylalanine into phenylalanine through enzyme activity assay. the n - carbamoylase activity of recombinant was 2. 26 and 2. 15 times higher than that of arthrobacter bt801 and dh5a / puc18 - 169

    將hyucdna片段連接到真核表達載體ppic3 . 5k上，經bgl酶切線性化，通過peg法轉化導入畢赤酵母gs115感受態細胞，利用g418抗性篩選得到12個插入多拷貝目的基因的轉化子。
  15. The labeled cdnas were used to hybridized with the prepared microarray. by using the dna microarray scanner and analysed with the quantarray software package, gene expression profiles of s. cerevisiae in response to heat were studied preliminarily

    最後提取熱休克前後酵母細胞的總rna ，經逆轉錄標記合成cdna后與酵母基因表達譜晶元雜交，初步研究了酵母細胞在熱應激時基因表達的變化。
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