毒性基因 的英文怎麼說

中文拼音 [dúxìngjīyīn]
毒性基因 英文
toxophore
  • 毒 : Ⅰ名詞1 （對生物體有害的性質或物質; 毒物） poison; toxin 2 （毒品） drug; narcotics 3 （姓氏） a s...
  • 性 : Ⅰ名詞1 （性格） nature; character; disposition 2 （性能; 性質） property; quality 3 （性別） sex ...
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 毒性 : [藥理學] toxicity; virulence; poisonousness毒性測定 toxicity test
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  3. Characterization of hemagglutinin gene of influenza a virus subtype h9n

    毒株基因組特性的研究
  4. Expression of thermostable direct hemolysin gene of vibrio parahaemolyticus in e. coli and activity of expressed product

    副溶血性弧菌直接溶血毒素基因的原核表達及產物的性質
  5. We have reported that squalene, a natural fish oil, is a potential preventive agent against sodium arsenite - induced sister chromatid exchanges and micronuclei in chinese hamster ovary - k1 cells

    以達成熟地步，因此使得探討環境毒物暴露引發的差異性基因表達成為可以進行的研究。我們已開始以
  6. To dress the question if other virulence gene were present in this kind of strains, 152 of 436 irp2 - hybridized strains were re - confirmed and selected for this study. the virulence genes or putative virulence genes detected by pcr or hybridization include heat stable toxin ( st ) & heat labile toxin ( lt ) for enterotoxigenic e. coli ( etec ), invasive plasmid antigen b ( ipab ) for enteroinvasive e. coli ( eiec ), epec adherence factor ( eaf ), epec secretion protein c ( espc ) for enteropathogenic e. coli ( epec ), hemolysin ( hlya ) and shiga toxins ( sltl and slt2 ) for enterohaemorrhagic e. coli ( ehec ) and eaggec probe for entero - aggregative e. coli ( eaggec ). the prra and yc73 genes of pathogenicity associated island ( pai ) of urepathogenic e. coli ( upec ) and " o " island 28 ( rtx 615 ) gene was also detected, the later was a newly discovered putative pathogenicity island in e. coli o157 : h7

    為探討攜帶小腸結腸炎耶爾森氏菌的hpi毒力島的大腸桿菌是否具有其他已知的毒力基因，選取82株由原位雜交和pcr方法初篩irp2陽性的大腸桿菌菌株，進行在致瀉性大腸桿菌的25個毒力基因的檢測，包括腸產毒性大腸桿菌的熱穩定毒素st和熱不穩定毒素lt ，腸侵襲性大腸桿菌的侵襲蛋白b基因ipab ，腸致病性大腸桿菌的eaf 、 espc基因，腸出血性大腸桿菌的溶血素hly 、志賀毒素1 （ slt1 ） 、志賀毒素2 （ slt2 ）基因，腸集聚性大腸桿菌的eaggec探針，以及在泌尿道致病性大腸桿菌和o157 ： h7大腸桿菌中新發現的毒力島基因。
  7. In this study, the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome, which have the lacz reporter gene, earlier / latter promoters lp2ep2 of fpv, promoters p7. 5 and p7. 1 of vaccinia virus, replication unnecessary region of fpv - 017. following 6 cycles screenings, clonings, purification of blue plaques, detection of pcr and dot - elisa, which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully. this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine, as well as provided substance preparatory for prevention the high mortality gpv

    本研究採用脂質體轉染方法，將含有完整gpvh1分離株vp3基因、報告基因lacz 、禽痘病毒早晚期啟動子lp2ep2 、痘苗病毒啟動子p7 . 5 、 p11和fpv - 017復制非必須區的轉移載體質粒psy681vp3lacz與fpv - 017共轉染雞胚成纖維細胞，經6輪蝕斑克隆、篩選、表達， pcr鑒定和dot - elisa檢測，證明該重組病毒已構建成功，並獲得了遺傳性狀穩定的鵝細小病毒vp3基因的重組禽痘病毒。
  8. Importance of five genes presented in xenorhabdus nematophilus bp toxin gene cluster to its insecticidal activity

    品系殺蟲毒素基因簇中各基因與殺蟲活性的關系
  9. Vp53 / vp37 genes have similar positions in rna2 to 58 - kd / 48 - kd movement protein ( mp ) genes of cowpea mosaic virus ( cpmv ) and encoded conserved motif of " 30k super group " mp

    通過與同科的豇豆花葉病毒（ cpmv ）基因組編碼區的比較發現， vp53 vp37與cpmv58 - kd 48 - kd移動蛋白的編碼位置相同並存在一定同源性，並且vp53 vp37內含有病毒移動蛋白特有的「 30k超組」保守模體。
  10. The expression conditions of e2 gene in p. pastoris were optimized, the results indicated that the peak obtained after 72 hours ; pattern of inhibition / induction could improve expression level ; the best ph value were between 7. 5 and 8. 0 and the optimized methanol - induced concentration was 2 % - 3 % the e2 genes of the prevalent strain ( guangxi yulin strain ) and c strain derived from rabbit spleen tissue were amplified and cloned into e. coli the expression vector pproex - htb respectively, the recombinant plasmids pproex - gxyl and pproex - c were obtained and then were transformed into the dh5a e. coli competent bacteria respectively, the recombinant bacteria could express the major antigen region of e2 gene, the expression yields amount to 35 % and 38 % repectively

    豬瘟病毒ez基因的原核表達： pcr擴增出當前豬瘟流行野毒株，中國豬瘟兔化弱毒（ c株）兔脾組織毒ez基因的主要抗原區，將其克隆到原核大腸桿菌表達載體pproex htb中誘導表達，經sds page檢測表明，重組質粒能表達ez基因主要區蛋白， westernblot檢測表明，誘導表達蛋白與豬瘟陽性血清發生特異性反應，表達量為35和38 ，可用於基因工程診斷抗原。
  11. This strain ' s virulence was judged by mean death time of chick embryos ( mdt ), intracerebral pathogenicity index in day - old chicks ( icpi ) and intravenous pathogenicity index in 6 - week - old chickens ( ivpi ) and it was found to be the virulent strain. at last, it was tested by the recurrent infection and found that it was the newcastle disease virus ( ndv ), and it was named hbg - 1 strain. in order to find the difference of the cleavage site of this strain with f48e9 and ? 30 strain, a part of the cleavage site of fusion protein gene fragment was amplified by rt - pcr using a primer and sequenced. the sequence analysis showed this strain had low homology with f4ge9 and cso. a phylogenetic tree based on the published sequences of ndv reference strains was constructed and showed the isolated strain hbg - 1 belonged to the genotype w ndv, a novel genotype ndv

    為了進一步探尋分離株與標準株的異同，又採用rt - pcr方法，擴增獲得分離株f _ o裂解位點附近的基因片段，經測序后與國際上已發表的新城疫病毒的核酸序列進行比較，結果表明其與標準株和疫苗株之間的同源性較低，僅為82 86之間。經系統發育進化樹分析后，判定該分離株為新城疫病毒（ ndv ）基因型。運用計算機軟體對其裂解位點處的氨基酸序列進行預測和分析，結果表明該分離株為新城疫病毒強毒株並具有基因型的典型結構特徵。
  12. With the aim of increasing the expression and stability of mtxl, the mtxl gene originated from b. sphaericus ssii1 was cloned to a shuttle vector pbu4. two recombinant plasmids pmt9 and pmt4 were obtained, with the inserted fragments in the opposite orientations

    為了提高mtx1殺蚊毒素蛋白的表達量和穩定性，本文將來源於球形芽孢桿菌b . sss - 1的mtx1毒素基因克隆至穿梭載體pbu4上，得到mtx1插入方向相反的重組質粒pmt9和pmt4 。
  13. The results suggested that eaggec hpi - positive strains expressed the ybt system, but the presence of hpi core part does n ' t mean ybt expression. 2. research on the function of ybtp, ybtq and ybtx genes in eaggec 17 - 2 ybtp, ybtq and ybtx genes from yen wa were cloned respectively and mutated, then inserted by a selectable substitution with chloramphenicokcaf ) gene in vitro

    攜帶hpi毒力島的eaggec17毛ybtp 、 ybtq 、 ybtx基因功能研究克隆了小腸結腸炎耶爾森菌hpi毒力島一rr7和叩結構基因，在體外進行精確突變后，插入氯黴素抗性基因（ cat ）作為選擇性標記。
  14. Analysis on the variance of vaca genotypes and their vacuolating toxin activity of helicobacter pylori isolates in zhejiang area

    浙江地區幽門螺桿菌臨床菌株空泡毒素基因的變異及其表達活性分析
  15. All these results suggested that the cause of 4d1 culture inhibiting the pathogenicity of scg1 is the decreased concentration of ahl molecule in scg1 cells, when presenting aii protein in 4d1 cells, and then not inducing the expression of virulence genes of scg 1 at low concentration of ahl molecules

    由此推測，蘇雲金芽胞桿菌影響病原菌致病性的原因在於降低了病原菌細胞內ahl類信號分子的濃度，使其達不到激活毒素基因表達的水平。
  16. These results showed that the three isolates were infectious bursal disease virus. the full - length cdna of the genomic segment a of two viruses, one virulent field strain ibdv zj2000 and one attenuated strains ibdv jd1, were amplified in a single step procedure by long - accurate reverse - transcription polymerase chain reaction ( la - pcr ), cloned into pgem - t easy vector, and sequenced

    分別以傳染性法氏囊病病毒zj2000株（野毒株）和jd1株（弱毒株）基因組dsrna為模板，採用long - accuratert - pcr （ la - pcr ）一步法擴增並克隆了兩株病毒的基因組a節段全長cdna 。
  17. The genome could be digested only by restriction endonucleases hind, accl and xbal, and was resistant to cleavage by a wide range of other restriction endonucleases

    病毒的基因組可以被hind 、 acc和xba酶切消化，但不能被其他所使用的限制性內切酶消化。
  18. " our exhaustive testing of amoy gardens found no genetic material or footprint of the sars related coronavirus in swabs taken from the affected apartments, the entrance between the units, the corridors, the roof, the ground floor and the sewer manholes

    小組在淘大花園受影響的單位、單位之間的入口、走廊、天臺、地下及排污沙井抽取樣本，進行全面測試，並無發現引起嚴重急性呼吸系統綜合癥的冠狀病毒的基因殘餘。
  19. The signal peptide sequence which was used for secretedly expressing divergent gene in mammary gland cells was ligated to the e2 gene, the e2 gene with signal sequence was obtained by pcr. the gene resisting kanamycin was cut down from pgfp - cl vector and inserted into p22 vector, a p22 vector with gene resisting kanamycin was constructed, it was tried to construct an expression vector for transforming go

    豬瘟病毒ez基因的乳腺特異表達載體構建：將在乳腺細胞中特異分泌的信號肽序列連接到ez基因， pcr得到了目的片段，再將pgfpci載體上的kana基因切下與在乳腺細胞中特異表達的p22載體連接，使其帶有篩選標記，然後將帶有信號肽的ez插入到p22中，試圖構建山羊乳腺上皮細胞的特異性表達載體。
  20. In this study, the recombinant plasmid pmd - 18t - pea - h3 was cleavaged with ncoi, xhoi and inserted into the expression vector pet - 28c and subsequently subjected to restriction endonuclease analysis and sequencing, the result indicated that the prokaryotic expression vector pet - 28c - pea - h3 was constructed successfully. after the expression plasmid was extracted and transformed into expression hosts bl21 ( de3 ) of e. coli, the transformed hosts were induced by iptg, bysds - page and elisa analysis of host protein. the expression of the objective gene was detected, and it could account for 16. 28 % of the total host protein. inclusion body was prepared from the incubating expression hosts induced by iptg

    同時將原核表達載體pet - 28c用nco ， xho雙酶切，回收酶切產物，將回收的酶切產物pea ， h3 ，載體進行連接，並轉入dh5感受態細胞內，培養12 - 18小時后，挑取陽性菌落，經nco ， xho雙酶切分析及pcr檢測，篩選到陽性克隆，其質粒測序結果表明成功地構建了毒性基因缺失的pea與人組蛋白h3融合基因的原核表達載體。
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