毒粒蛋白 的英文怎麼說

中文拼音 [dúlìdànbái]
毒粒蛋白 英文
virion protein
  • 毒 : Ⅰ名詞1 （對生物體有害的性質或物質; 毒物） poison; toxin 2 （毒品） drug; narcotics 3 （姓氏） a s...
  • 粒 : Ⅰ名 （小圓珠形或小碎塊形物） small particles; grain; granule; pellet Ⅱ量詞（用於粒狀物）
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  1. First, to construct a recombinant plasmid pegfp - c - fos with c - fos promoter and egfp, and then transfect it into human bladder transitional cell carcinoma biu - 87 cell ; second, based on the changes of the expression of gfp in the biu - 87 cell which induced by the aconitine and hab toxins, the concentration of the hab toxins could be detected

    目的：構建一個含c - fos啟動子和egfp報告基因的pegfp - c - fos重組質粒載體。體外轉染膀胱癌biu - 87細胞后，利用赤潮毒素作用后細胞表達綠色熒光蛋白的變化來檢測赤潮毒素，初步建立一種以細胞為基礎受體水平的赤潮毒素檢測方法。
  2. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  3. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  4. A virion consists of a protein coat surrounding one or more strands of dna or rna

    一個病毒粒子包括外部的蛋白衣殼和內部的一條至多條dna或rna鏈。
  5. To investigate whether ha 132 is a structural component of hasnpv, western blot analysis of proteins in budded viruses ( bvs ) and occlusion derived virions ( odvs ) was conducted

    對來自bv和odv的總蛋白質進行westernblot分析，結果表明ha132蛋白不是hasnpv病毒粒子的結構成份。
  6. Transfection of cell lines impaired for vzv infection with a plasmid expressing human ide resulted in increased entry and enhanced infection with cell - free and cell - associated virus

    Vzv感染受抑制的細胞系通過質粒表達轉染人類ide蛋白后可增加游離病毒顆粒以及細胞內病毒引起的感染。
  7. 2. an up - dated method was employed to purify tumv in this research. using the protease k method, we acquired the viral genome - rna. a pair of specific primers was designed and synthesized based on the nucleotide sequences of tumv coat protein genes reported before and rt - pcr was used to clone the cp genes of the six tumv isolates

    應用改進的蕪菁花葉病毒的提取方法從病葉中提取病毒粒體，應用蛋白酶k法從病毒粒體中提取病毒rna基因組，根據已報道的tumv的cp基因序列，設計併合成了一對特異引物，利用rt - pcr法克隆了6個分離物的外殼蛋白基因，與克隆載體puc19連接后通過熱激法轉化大腸桿菌dh5 。
  8. Vp3 gene of hl isolate of goose parvovirus derived from recombinant piasmid pproex htb - vp3 was subcloned into ecorl site of psy538, and the reporter gene lacz with promoter pll was cloned into smai site of recombinant piasmid. both vp3 gene and lacz gene were cloned into noti site of psy681, recombinan fpv transfer vector containing vp3 gene of gpv was obtained. the result is basis of construction of recombinant fpv expressing vp3 gene of gpv and gpv genetic engineering vaccine

    本研究另從含有gpvh1分離株主要結構蛋白vp3基因的重組質粒pproexhtb - vp3中切取gpvh1株vp3基因片段，將其亞克隆于psy538的ecori位點，然後將帶有痘苗病毒啟動子p11的lacz報告基因平端克隆于上述重組子的smai位點，再用noti切下同時含有vp3基因和lacz報告基因的片段，再亞克隆于psy681的noti位點，構建出含有vp3基因的重組禽痘病毒轉移載體，為構建表達vp3基因的重組禽痘病毒從而制備gpv基因工程疫苗奠定了基礎。
  9. Salmonella typhimuriwn, one of the invasive bacterial species, can be attenuated without loss of invasiveness and thus used for delivery of eukaryotic expression vectors into host cells in vivo. the recombinant plasmid containing the target gene is released inside the host cells and gain entry into the nucleus, resulting in expression of encoded antigens and subsequent induction of humoral and cellular immune responses

    沙門氏菌（ salmonellatyphimurium ）是一種較為常見的侵襲性胞內菌，通過基因工程方法減毒后對宿主致病性顯著降低，但仍保留良好的侵襲力，可直接將真核表達質粒攜帶進入動物細胞內表達相應的蛋白而誘導特異性的免疫應答反應。
  10. Further sequence analysis show that only 6 base pairs of nucleotide and 2 amino acids are different between them. the homological cry3aa gene was expressed in escherichia coli. and the expressed products which contain a fused peptide of 66 - 97 kilo - dalton was observed by means of sds - page

    生物活性測定結果表明該菌株對榆藍葉甲（ pyrrhaltaaenescens （ fairmaire ） ）和光肩星天牛等鞘翅目昆蟲具有較高的毒力，因此初步確認該菌株屬于cry3類； （ 2 ）發現該菌株中編碼毒蛋白的基因位於質粒上，並且已經成功地克隆到該基因。
  11. Thin sections of host leaf cells infected by bbwv - 2 isolate b935, which were gold - labeled by antibodies of bbwv - 2 coat protein ( cp ) and vp37, respectively, were prepared to elucidate the locations of vp37 in cell and possible function of vp37 and cp in cell to cell movement. observation in electron microscope showed that virus particles were presented not only in cytoplasma but also in chloroplast, while vp37 was existed only in cytoplasma and associated with tubular structure through the cell wall

    為研究vp37在寄主細胞中的作用機制及其在細胞中的分佈，通過膠體金間接標記6his - vp37兔抗血清，同時還標記了病毒的外殼蛋白單克隆抗體，對bbwv - 2分離物b935感染的病葉超薄切片的電子顯微鏡觀察發現：病毒粒子除了聚集在胞質中，還存在於寄主的葉綠體內； vp37蛋白能在細胞壁上形成管狀結構，在胞質中亦有分佈。
  12. In this work, effects of sfamnpv on mitochondrial and er in sl - 1 cells were studied. the main results were as follows : effect of sfamnpv ( moi = 6 ) on the function of mitochondrial in sl - 1 cells was investigated by mtt assay and membrane potential was assayed by both flow cytometry and confocal laser scanning microscope

    完成的工作包括以下幾個方面： （ 1 ）細胞內線粒體的變化通過mtt法研究病毒感染對細胞線粒體功能的影響，同時用流式細胞儀和共聚焦激光顯微鏡研究線粒體膜電位（ m ）的變化， western檢測2種凋亡調控蛋白cytoc和bcl - 2蛋白的變化。
  13. As peptide glycoproteins react with viral particles leaving infected cells

    作為離開被感染細胞的病毒粒子起作用的勝肽聚醣蛋白。
  14. Thus, the success of nscs therapy is determined by the induced differentiation and the effect of micro - enviroment on the survival, proliferation and differentiation of it. the isolation and culture of nscs in vitro successfully is the presupposition for its research. to avoid the influence of unknown factors exiting in serum on nscs " differentiation, serum - free medium with egf and bfgf as mitogens to stimulate proliferation and inhibit differentiation in vitro was introduced through a long period of search

    澱粉樣蛋白（ amyloidpeptidy ， a ）是澱粉樣蛋白前體蛋白（ amyloidprecusorprotein ， app ）異常代謝產生的含有39 ? 43個氨基酸殘基的疏水肽，可聚合成不溶的纖維形式或不定型顆粒狀結構，導致各種毒性反應，在阿爾茨海默病（ alzheimer ' sdisease ， ad ）發病中起著至關重要的作用。
  15. Lymphotoxin ( lt ) is a kind of pleiotropic lymphocyte - secreted cytokine which mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. in order to increase the antitumor activity of lymphotoxin and reduce its side effects, the recombinant plasmid pet36b - lt 27 was constructed to express soluble fusion protein cbd - lt 27. the active form of lt 27 could be collected directly with several simple steps by three kind of components on the expressed fusion protein

    本研究通過構建表達n端缺失27個氨基酸的淋巴毒素融合蛋白的重組質粒，在大腸桿菌中實現融合蛋白的可溶及分泌表達，同時利用表達載體上的幾種特殊序列經簡單的分離純化步驟直接獲得大量的有生物活性的淋巴毒素缺失體lt 27 ，為尋找一種高抗腫瘤活性、低臨床毒副作用的生物抗癌藥物進行了有效的探索。
  16. A human lymphotoxin deletion gene fragment which lacking n - terminal 27 amino acid residues of the protein was pcr amplified using a pair of designed primers and cloned into expression vector pet36b ( + ) to construct a fusion protein with cbd tag, resulting a recombinant plasmid pet36b - lt 27. the recombinant plasmid was transformed into host e. coli bl21 ( de3 ) plyss

    採用pcr的方法擴增出人淋巴毒素n端缺失27個氨基酸的缺失體基因片段，將此基因片段克隆至表達載體pet36b （ + ）上，與t7lac啟動子控制下的cbdtag構建成表達融合蛋白的重組質粒pe736b - lt 27 。
  17. The expression conditions of e2 gene in p. pastoris were optimized, the results indicated that the peak obtained after 72 hours ; pattern of inhibition / induction could improve expression level ; the best ph value were between 7. 5 and 8. 0 and the optimized methanol - induced concentration was 2 % - 3 % the e2 genes of the prevalent strain ( guangxi yulin strain ) and c strain derived from rabbit spleen tissue were amplified and cloned into e. coli the expression vector pproex - htb respectively, the recombinant plasmids pproex - gxyl and pproex - c were obtained and then were transformed into the dh5a e. coli competent bacteria respectively, the recombinant bacteria could express the major antigen region of e2 gene, the expression yields amount to 35 % and 38 % repectively

    豬瘟病毒ez基因的原核表達： pcr擴增出當前豬瘟流行野毒株，中國豬瘟兔化弱毒（ c株）兔脾組織毒ez基因的主要抗原區，將其克隆到原核大腸桿菌表達載體pproex htb中誘導表達，經sds page檢測表明，重組質粒能表達ez基因主要區蛋白， westernblot檢測表明，誘導表達蛋白與豬瘟陽性血清發生特異性反應，表達量為35和38 ，可用於基因工程診斷抗原。
  18. Haake da, champion ci, martinich c, et al. molecular cloning and sequence analysis of the gene encoding ompl1, a transmembrane outer membrane protein of pathogenic leptospira spp [ j ]. j bacteriol, 1993, 175 ( 13 ) : 4225

    晏菊芳，鮑朗，伍衛華，等.中國鉤端螺旋體強毒株017膜蛋白基因的質粒載體構建及表達[ j ] .中華微生物學和免疫學雜志， 1999 ， 99 （ 2 ） : 117
  19. Different hosts " response suggested that tumv - sd1 could infect plants of 10 species in 3 families. tumv - sd1 formed pine - wheel inclusion bodies in plant cells. the coat protein of the tumv - sd1 contains 3 components whose estimated molecular weight are 45kd, 38kd and 14kd respectively

    寄主反應特性表明， tumv - sd1 6能侵染3科10種植物， tumv - sd1在寄主細胞內形成風輪狀內含體，外殼蛋白為3組分，分子量分別為45kd 、 38kd和14kd ；提純的病毒粒體為長線條狀。
  20. With the aim of increasing the expression and stability of mtxl, the mtxl gene originated from b. sphaericus ssii1 was cloned to a shuttle vector pbu4. two recombinant plasmids pmt9 and pmt4 were obtained, with the inserted fragments in the opposite orientations

    為了提高mtx1殺蚊毒素蛋白的表達量和穩定性，本文將來源於球形芽孢桿菌b . sss - 1的mtx1毒素基因克隆至穿梭載體pbu4上，得到mtx1插入方向相反的重組質粒pmt9和pmt4 。
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