比抗性 的英文怎麼說
中文拼音 [bǐkàngxìng]
比抗性
英文
specific resistivity-
Diplomats here this week say the atmosphere is relaxed, non - confrontational and more positive than the three previous rounds of negotiations held since 2003. they ended in increased acrimony and no progress
在北京的外交界人士本星期說,同2003年以來舉行的前三輪會談相比,這次會談的氣氛比較輕松,對抗性比較小,也更加積極。在前三輪會談中,由於氣氛越來越緊張,最終都沒有取得進展。The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中,通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中,然後採用葉盤法,在該農桿菌的介導下將pap基因導入普通煙草中,經過卡那黴素抗性篩選,最後獲得了轉pap基因的工程煙草植株,摩擦接種煙草花葉病毒( tmv ) ,與非轉基因煙草相比,能夠推遲癥狀表現達2月之久,說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。These results suggested that higher accumulation of ergosterol might be induced by imazalil in the isolate of pd07, which contained 4 - 126bp tandemly repeated in cyp51 gene more than that in the pd23
與敏感菌株相比,抗性菌株cyp51基因啟動子上游多了4個126bp的串聯重復序列,推測該重復序列可能是導致cyp51基因過量表達的內在原因。In order to analyze the differences of the content of ergosterol in the imazalil - sensitive and imazalil - resistant isolates of p. digitatum, the technology of hplc was used. the results showed that the contents of ergosterol between imazalil - sensitive and imazalil - resistant isolates were not significantly defferent ( p = 0. 05, dmrt ) when they grew in the medium without imazalil. interestingly, when imazalil ( 0. 1 # g / ml ) was added to the medium, the content of the ergosterol in imazalil - resistant isolate ( pd07 ) was significantly higher than that in imazalil - sensitive isolate ( pd23 ) ( p = 0. 05, dmrt )
結果表明,在抗性菌株pd07不加抑霉唑的情況下, pd07和pd23中游離麥角甾醇的含量採用duncan ' s新復極差測驗在5水平沒有明顯差異;但當pd07中加入0 . 1 g ml抑霉唑時,三次重復測定結果顯示抗性菌株pd07中麥角甾醇的含量明顯地要比敏感菌株pd23中的含量高,而且兩者在5水平上差異顯著。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中,轉化大腸桿菌dh5菌株,篩選氨芐青霉素抗性菌落,提取質粒經酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原核表達載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導表達,收集菌液進行sds - page電泳、 westernblotting分析,結果表明, 3ab基因在大腸桿菌中成功表達,其表達產物為分子量33 . 5ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總蛋白量的26以上。Sensitive varieties apparently respire mainly by the glycolysis pathway whereas resistant varieties depend more on the pentose phosphate pathway.
顯然敏感品種的呼吸主要通過糖酵解途徑,而抗性品種比較依賴戊糖磷酸途徑。It revealed that these two transformants were heterozygote, and the foreign gene inserted just to on locus in the plant genome. as the observed ratio of progeny of two transformants which on selfing was 3 : 1, it was proved to be heterozygote for two transformants
其中2棵抗性株自交後代的遺傳分析表明,真空滲入法所得的轉化株為雜合體,且其分離比符合3 : 1的單基因分離規律。The tl seeds are screening on the ms medium which contains 50ug / ml kan, and there are about 70 % of the transgenic lines showed the kan - resistance with a ratio of 3 : 1. however, the other lines did n ' t show the ratio consistent with the mendelism. it might prove that most of the transgenic plants only have one insert
對收獲的轉基因番茄t _ 1代種子進行卡那黴素抗性篩選,結果發現, 70以上獨立轉化株系的t _ 1代種子出現了3 1的抗性分離,另一些獨立轉化株系的種子並未表現出符合孟德爾遺傳的分離比,說明大部分er - shsp基因是以單拷貝的方式插入植物基因組。Through indoor routine tests, the physical and mechanical natures of intact and remolding soil of muck and mucky soil, in fuzhou, are analyzed in this thesis. the results reveal that water content, void ratio, shear strength of remolding soil samples are lower than those of intact samples. then qualitative analysis on the mineral composition, chemical composition and microstructure characteristic and types of the soft clay is given
本文通過室內常規試驗對福州地區淤泥及淤泥質粘土的原狀土樣和擾動土樣的物理力學性質進行了分析,觀察土樣受擾動后一些物理力學指標的變化情況,發現擾動軟粘土樣的含水量、孔隙比、抗剪強度指標均比原狀土樣低;並從軟粘土的礦物成份、化學成份以及微觀結構特徵和類型上定性地分析。In this study, genetic diversity of various strains of spirulina platensis is analyzed with rapd molecular marker and antibiotics marker, the results are compared with their morphologic characters, aimed at constructing a more reasonable classification criterion and knowing more about the genetic background of spirulina. additionally, it is found that under the cultivated condition the linear filament can retransite to the normal curved one which is similar to original spiral filament
本論文利用rapd分子標記及抗生素抗性標記分析了不同鈍頂螺旋藻品系的遺傳多態性,並與其形態學特徵進行了比較,以期建立更合理的螺旋藻分類方法及加深對螺旋藻遺傳背景的了解;同時,發現在養殖環境下變直藻絲體可以回變為正常螺旋形態的現象。10. after polluted by so2, both chlorophyll and protein content in resistant plant leaves and sensitive plant leaves decrease. the higher so2 concentration, the bigger decreased rate, and the decreased rate of sensitive plant is obviously bigger than resistant plant
10 .在受到50 :污染后,杭性和敏感植物葉片中葉綠素和蛋白質的含量均有所降低,且50 :濃度越高,下降的比率越大,但敏感植物的下降比率顯著大於抗性植物。8. after fumigation for 3h in diverse concentration so2, the cat activity of resistant plant and sensitive plant is obviously less than the check group, and the rate of cat decrease rises with the rise of so2 concentration. the cat activity of resistant plant is comparatively stronger than sensitive plant ' s
8 .不同濃度的50 :熏氣3h后,抗性和敏感植物體內的cat活性均明顯低於對照,而且隨50 :濃度的增大cat降低的比率也隨之增大,濃度越大,差別越顯著,而且抗性植物體內的cat酶活性相對高於敏感植物。Detected resistance ratios of the adults in bemisia tabaci to imidacloprid, acetamiprid and thiamethoxam were respectively 1. 22, 3. 28 and 2. 49 by using adult leaf - dip bioassay, and 1. 37, 4. 27 and 3. 26 by using systemic bioassay, which showed the same resistant level detected by two bioassay methods
摘要採用浸葉法和內吸法測得的煙粉虱成蟲對吡蟲啉、啶蟲脒和噻蟲嗪的抗性比值分別為1 . 22 、 3 . 28 、 2 . 49和1 . 37 、 4 . 27 、 3 . 26 , 2種方法測定的結果基本一致。Carboxylesterase activities of the brown and the whitebacked planthoppers feeding on resistant rice varieties
水稻抗性品種對褐飛虱和白背飛虱羧酸酯酶活力影響的比較研究The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides
進一步對xynba進行了脫糖基化處理得到xynbb ,其分子量恢復到23kd ,證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現:三種酶的最適ph差異不大, xynb和xynba均為5 . 2 , xynbb為5 . 0 ; xynb和xynba的最適溫度均為60 , xynbb降為50 :在耐熱性上, xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ; xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ,明顯低於原酶的比活2814 . 45iu mg ; xynb和xynba的km值相當,分別為21 . 56 ( g kg )和20 . 87 ( g kg ) ,而xynbb的km值較大為27 . 10 ( g kg ) ; xynba和xynbb的vmax相差不大,分別為4568 mol mg ? min和5329 mol mg ? min ,明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性,對胃蛋白酶和胰蛋白酶有很好的抗性,且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現:以樺木木聚糖為底物時,酶解產物主要為木三糖和木四糖,含量分別為68 . 43和16 . 50 ,另外還含有11 . 79的木二糖;以玉米芯木聚糖為底物時,酶解產物主要為木二糖和木三糖,含量分別為81 . 78和11 . 55 。Increased ratio of reducing sugar to soluble nitrogen is associated with genetic resistance of peas to acyrthosiphom pisum.
還原糖與可溶氮比率的增加同豌豆蚜蟲的遺傳抗性有關。The study shows that the early and late seed rains of constructive tree species in evergreen broadleaved forest at chongqing simian mountain had no activity. the bigger the seeds of the species and the earlier or later the seeds of the species matured, the higher the proportion of the seed rain damaged by animals. the quantitative variation of seed rain, active seed rain and seed bank did not take place at the same time. at early time, the number of seed banks of castanopsis fargesii, lithocarpus glabra, quercus myrsinrefolia, castanopsis plasyacantha, cinamomum subavenium. which own more active seed rain increased by geomitric series. the seed banks of castanopsis orthacantha and schima argentea were small, only survived a short time, and did not sprout next year. the quantitative dynamics of seed banks and their increasing or decreasing rates were decided by the proportion damaged by animals, dying speed of seeds and their resistance to pathogens and adverse circumstances, and the state of seed germination
對重慶四面山常綠闊葉林建群種種子雨、種子庫的研究表明,建群種早期和晚期的種子雨無活力;種子偏早或偏晚成熟及大籽粒的樹種,其種子雨被取食的比例大;種子雨、有活力種子雨、種子庫三者的數量變化不一致;有活力種子雨量較大的栲、石櫟、小葉青岡、扁刺栲、香桂等,其種子庫密度在早期以近幾何級數的方式增長,元江栲、銀木荷種子庫小,存在時間短,翌年無一年生萌發苗;種子庫數量動態、消減率動態決定於種子被取食的強度、種子衰老的速度以及種子對病菌、逆境的抗性和種子萌發的整齊性The tissue distribution of monooxygenase activities of cotton bollworm, helicoverpa armigera
氰戊菊酯和苯巴比妥鈉對敏感和抗性棉鈴蟲中腸多功能氧化酶系的誘導作用In this paper, the writer explores to make it clear for people to understand the criminal offence in conspiracy by untypical underworld society in four aspects including the conception and basic features of the untypical underworld society, the component elements of the criminal offence in conspiracy by untypical underworld society, the affirmation of such criminal offence and the penalty upon it, in the light of theoretical research and positive analysis. the conception of the untypical underworld society in china is summarized in comparison with the notion of " society ", " untypical underworld society " and the " features " of the untypical underworld society in china. the writer elaborates, when describing the features of the untypical underworld society, such features as " highly - organized ", that " there exists a sphere of influence in a certain district, or in a certain trade or profession, and illegal control is executed within the sphere of influence ", " with actual economic strength in pursuit of economic benefits ", " political infiltration into governmental organs with antagonism ", " decaying culture " and " the compatibility in the measures of criminal offence "
在論述黑社會性質組織的概念時,結合「社會" 、 「黑社會」的特點和我國黑社會性質組織中「性質」的來源,最後概括出我國黑社會性質組織的概念;在論述黑社會性質組織的特徵時,本文從黑社會性質組織所具有的「比較高的組織化程度」 、 「在某一地區或某些行業具有一定的勢力范圍,並在該范圍內形成非法控制」 、 「以追求經濟利益為主要目的,具有一定的經濟實力」 、 「對政府的滲透,政治上的對抗性」 、 「文化上的腐朽性」 、 「犯罪手段上的兼并性」等方面進行闡述,特別是在「對政府的滲透,政治上的對抗性」採用大篇幅論述,並提出國家工作人員的「保護傘」既包括「包庇」又包括「縱容」 ,特別是在「縱容」方面提出了一些新的觀點;在論述黑社會性質組織犯罪構成特徵時,從犯罪的主體、侵害的客體、犯罪的客觀方面、主觀方面進行了詳細敘述;在論述黑社會性質組織犯罪的認定時,主要從黑社會性質組織與相關范疇的界限、黑社會性質組織罪的司法認定兩個方面來進行的;在論述黑社會性質組織犯罪的刑事處罰時,提出了對各種組織、領導、參加黑社會性質組織行為的處罰原則,並對提高該罪的量刑幅度及增設財產刑提出了立法建議。6. the plant is comparatively sensitive in two leaves period. so we can use the dose reaction to so2 in two leaves period as the injury threshold value to classify resistant ability type
6 .植株在2片真葉期對50 :比較敏感,因此可以採用植物在2片真葉期對50 :的劑量反應作為受害閡值來劃分抗性類型。分享友人