毛基體基因 的英文怎麼說
中文拼音 [máojītǐjīyīn]
毛基體基因
英文
kinetogene-
The purposes of our work are to establish a simplified method of multiplex pcr based on chimeric primers for str loci, to develop a set of fluorescent quadriplex str system for forensic dna typing based on this method, and to validate the forensic application of the system under the guidelines of tmgdam ( the technology working group on dna analysis methods ) in order to address concerns presented in today ' s legal environment
目的本課題旨在探索一種新的str基因座復合擴增方法，我們稱為嵌合引物str復合擴增法。應用熒光標記毛細管電泳激光自動檢測技術平臺，建立一套新的法醫str基因座復合擴增體系，並按照美國dna分析方法技術工作組（ thetechnologyworkinggroupondnaanalysismethods ， twgdam ）的指導方案進行法醫學實用性研究。The results are agreeable with morphological taxonomy. the software mega was used to analyze the molecular phylogeny and phylogenetic trees were constructed with the neighbor - joining method and the maximum parsimony method
對所得的基因序列用mega軟體包進行系統進化分析， nj法和最大簡約法（ mp ）構建毛冠鹿和麂屬3種動物的分子系統進化樹。Introduce according to the relevant data, persian cat is in around 16 centurieses, from the himalayas cat and angola cat miscellaneous hand over, lift through several years to breed but grow purely. persian cat ' s figure is bigger, wering grown by the hair and thick and airtight, head circle big, the face is flat even, sum breadth the ear is small, circle the eye snub - nosed tone short breadth, the body feels round and smooth because the hair grows, the arms and legs is thick short soft, the tail is fluffy and bulky, giving person a kind of noblest felling. persian cat ' s pressing is canned is divided into a white, black, red by the hair color ' s dissimilarity, yellow, dark gray, blue, double color, tortoise shell color, miscellaneous color, tiger spot color etc. species. take species of the red as among them valuable
根據有關資料介紹，波斯貓是在16世紀左右，由喜馬拉雅貓和安哥拉貓雜交，經過多年的提純繁殖而培育出來的.波斯貓體形較大，被毛長而且厚密，頭圓大，臉扁平，額寬耳小，圓眼塌鼻口吻短寬，軀體因毛長而感覺渾圓，四肢粗短柔軟，尾蓬鬆粗大，給人一種雍容華貴的感覺.波斯貓按被毛顏色不同可分為白色，黑色，紅色，黃色，暗灰色，藍色，雙色，玳瑁色，雜色，虎斑色等品種.其中以紅色的品種尤為名貴.波斯貓外表美麗大方，叫聲細小甜美，性格溫和，膽大好奇，喜歡與人親近，善解人意，容易調教，是一種深受人們喜愛的高貴寵物.有關波斯貓的起源眾說紛紜，現較統一的說法是在阿富汗土著長毛貓的基礎上，同土耳其或亞美尼亞地區的安哥拉貓雜交培育而成。The result showed that the homology rate of pila gene among the 5 avian pathogenic e. coli strains tested and one human e. coli were from 89. 8 % to 91. 1 %, and the homology rate of amino acid were from 88. 5 % to 91. 8 %. the homology rate of pila gene sequence among 5 avian pathogenic e. coli strains tested and avian pathogenic e. coli reported ( serotype o1, o2, o78 ) were from 87. 8 % to 90. 2 %, and the homology rate of amino acid were from 84. 6 % to 91. 2 %. there had homology in avian pathogenic e. coli. there had some common antigen side in type 1 pili of avian pathogenic e. coli
結果表明：運用msha法檢測1型菌毛的檢出率為80 （ 36 45 ） ， pcr法的檢出率為95 . 5 （ 43 45 ） ， pcr方法用於1型菌毛的檢測比msha更加敏感、快速、特異性強；選擇5株優勢血清型雞源致病性大腸桿菌代表株（ o _ （ 89 ） ， o _ （ 119 ） ， o _ （ 141 ） ， o _ （ 127 ） ）的1型菌毛pila基因的pcr擴增片段經純化后，分別定向克隆到puc18質粒的多克隆位點，構建了含有目的基因片段的克隆質粒，並轉化到dh5株大腸桿菌載體菌中，篩選獲得陽性克隆菌株。Ceviar living cells, which undergo devision 200 times faster than normal cells, contain a great variety of activated factors that stimulate fission of both organism and cells, and regeneration of cells, improving micro circulation and supply of nutrients, reducing melanin, and thus growing vitality and skin springiness, resulting from less wrinkles and shrinking pores
魚子活細胞有旺盛的生命力，它的細胞分裂頻率比成熟細胞強200倍，內含多種活性因子，可啟動人體組織細胞的分裂和活化，刺激老化細胞更新再生，改善微循環，淡化黑色素，其高分子蛋白質有效的補充人體細胞和皮膚細胞的養份，增強機體活力和皮膚的彈性，修護鞏固基底層細胞組織達到去除皺紋、收緊毛孔、提升皮膚的顯著美容效果。In order to express alkaline protease gene ( ap gene ) in bacillus subtil is, the recombinant expression plasmid was constructed. this plasmid contains a promoter bp53, also from b. pumilus un - 31 - c - 42, ap gene and the shuttle vector psugv4. after introduced into b. subtilis wb600, the transformants displayed the hydrolyzed zone on milk plate
將來自短小芽抱桿菌un一31一c礴2的基因啟動子（ bp53片段）和脫毛蛋白酶全基因（ ap ）進行融合，然後將重組基因（命名為bpap ）插入到大腸桿菌-枯草桿菌穿梭質粒載體psugv4中，構建成表達質粒psu一bpap 。This promoter may provide efficient bioengineering to enhance pest and pathogen resistance, and is of theoretical significance on the trichome molecular system
此啟動子可用於基因工程提高植物體對外界侵害的抵抗力，對于研究表皮毛發育調控機理也有一定理論意義。We clone a 1. 3kb promoter sequence of the homologous gene in arabidopsis by pcr. this promoter is shown to direct the specific expression of the reporter gene, b - glucuronidase ( gus ), in trichomes of arabidopsis. promoter deletion analysis reveal that the region from - 300 - - 1 bp is sufficient to direct trichome - specific expression
對其進行缺失突變，構建5個缺失表達載體轉基因擬南芥，葉片gus定量測定分析表明- 300bp ? - 1bp序列就可以指導gus基因在表皮毛細胞中特異表達，說明這段序列可能含有指導此啟動子在擬南芥表皮毛細胞進行特異表達的核心序列。Sixties primers were used for dna amplification and a total of 237 amplification products range from 450bp to 2500bp were generated. a similarity matrix for all pairwise comparisons was calculated using nei and li ' s formula and then transformed to distance matrix. dendrograms were constructed by applying unweighted pair - group arithmetic average ( upgma ) and neighboring - jointing cluster analyses using the phylip software
在第二部分，應用改進的ctab法提取了石蓴屬和滸苔屬各3個種及作為對照的剛毛藻的基因組dna ， 60個引物被用於擴增，共獲得237個片段大小在450 - 2500bp之間的擴增片段，依據nei和li （ 1979 ）的公式計算出樣品成對比較的相似性距陣並換算成遺傳距離，應用phylip軟體包，按照upgma法和n - j法分別構建聚類圖。After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s
通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。All diodes have large reverse leak current density, which maybe caused by some reasons such as many ions are brought in course of evaporating metal on silicon surface of 6h - sic, chemical etch brings disfigurements such as burrs and dentate erodes as well as the rinse on surface of samples is not drastically accomplished
兩個肖特基二極體反向漏電流較大，估計原因為正面蒸發金屬時引入大量離子、光刻引入毛刺和鉆蝕等缺陷、金屬與樣品粘附能力差及樣品背面歐姆接觸制備好后正面清洗不充分等。Although the above example shows a simple relationship between two alleles located at one gene locus, coat color in dogs is ultimately quite complicated and not fully understood
盡管上述例子顯示了在（染色體上）同一個基因位置的等位基因的簡單關系，但最終決定狗的被毛顏色（的因素）是很復雜的，尚不能（為我們所）完全理解。In order to establish the genetic transformation system of saussurea medusa maxism by agrobacterium rhizogenes, some work were done. the main results were following : 1 establishment of regeneration systems two systems of regeneration from saussurea medusa maxim were established without cold treatment. the somatic embryos were induced from callus cultured in mise for 35 days. the shoots were induced from cotyledon after cultured in misc 20 days, and from leave which were cultured in misl. the experiment showed that the carbon and glycine in the medium could help to increase the regeneration rate to 95 %
為篩選水母雪蓮代謝突變體和轉基因研究奠定了一定的基礎，陳亞瓊： m質粒介導水母雪蓮的遺傳轉化及毛狀根中決刪合成的調節p義摘要也為研究類黃酮代謝途徑的關鍵酶基因的轉化、高效表達及作用機理提供了理想的實驗體系。主要的實驗結果如下： 1水母雪蓮高頻再生體系的建立通過體細胞胚胎發生途徑和器官發生途徑，水母雪蓮（ squssureamedusamaxim ）可以在常溫下獲得再生植株。The results suggested that eaggec hpi - positive strains expressed the ybt system, but the presence of hpi core part does n ' t mean ybt expression. 2. research on the function of ybtp, ybtq and ybtx genes in eaggec 17 - 2 ybtp, ybtq and ybtx genes from yen wa were cloned respectively and mutated, then inserted by a selectable substitution with chloramphenicokcaf ) gene in vitro
攜帶hpi毒力島的eaggec17毛ybtp 、 ybtq 、 ybtx基因功能研究克隆了小腸結腸炎耶爾森菌hpi毒力島一rr7和叩結構基因，在體外進行精確突變后，插入氯黴素抗性基因（ cat ）作為選擇性標記。With bacterial cgc as main subject, the tests had been done to elucidate mechanism of self - organization for macroscopic rhythmic structure. the dynamics of cgc forming was observed by special techniques of waving culture and microscopic culture ; the differences in outer structure of cell wall and flagella number had been observed by atomic force microscope scanning ; integrity of cell wall was examined under tem ; outer membrane protein was analysed by sds - page and various substance and factors for cgc formation were determined
採用特殊的波動培養和顯微培養技術觀察潛生體形成動態；應用原子力顯微鏡掃描，比較細菌潛生體與繁殖體在細胞壁外層結構和鞭毛數量的差別；用透射電鏡觀察細胞壁完整性，以十二烷基硫酸鈉?聚丙烯酰胺凝膠電泳分析外膜蛋白的改變，並通過實驗分析多種物質和因素對潛生體形成的影響。Result. a method of multiplex pcr based on both chimeric and universal primers was established. a fluorescent quadriplex str system, including d1s1612, d9s2026, d20s161 and d6s477, was developed on the basis of the multiplex pcr
建立了dis1612 、 d952026 、 d205161 、 d65477str基因座熒光標一記嵌合引物復合擴增毛細管電泳激光自動分析體系，同時證明嵌合引物復合擴增方法與毛細管電泳激光自動分析方法相兼容。Ts87 gene fragment was picked out by screening adult t. s cdna library using sera of rabbit raised against soluble antigen of t. s and using sera of rabbit infected artificially with t. s. the ts87 fragment was ligated to vector pcdnas. l, which contains a cmv promoter / enhancer
本研究採用通過免疫篩庫得到的旋毛蟲抗原基因片段ts87與載體pcdna3 . 1連接。該載體含有人類巨細胞病毒（ cmv ）的高效啟動子和增強子，是一種外源基因在哺乳動物細胞內高效表達的理想載體。To date insect resistance has been provided by a gene from the soil bacterium bacillus thuringiensis bt this gene directs cells to manufacture a crystalline protein that is toxic to certain insects especially caterpillars and beetles that gnaw on crops
這個基因會促使細胞製造一種晶體狀蛋白質，對某些昆蟲來說是毒藥，尤其是啃食作物的毛毛蟲和甲蟲，卻不會傷害其他生物。分享友人