氨化產物 的英文怎麼說
中文拼音 [ānhuàchǎnwù]
氨化產物
英文
aminate-
( 6 ) 4, 6 - diamino - 5 - nitrobenzofuroxan is designed to synthesis from trichlorobenzene through nitration aminatiom azidation and thermal decomposition. this compound is a new explosive. but we only got the intermediates of the first and second steps ( nitration, amination ) because the limit of time
( 6 )從均三氯苯出發,設計經硝化、氨化、疊氮化和脫氮四步合成4 , 6 -二氨基- 5 -硝基苯並氧化呋咱,這是未見文獻報道的新化合物,不過由於時間關系,我們只得到氨化這一步的中間產物即3 , 5 -二氨基- 2 , 4 -二硝基氯苯,這也是個未見文獻報道的新化合物。In the modern breeding, there are large amount of the excrement to be accumulated, these organic excrement will produce the large amount of ammonia and other harmful nitrogenous substance via ammonification
現代集約化養殖長期累積了大量養殖排泄物,這些有機排泄物經氨化作用產生大量氨等含氮有害物。Abstract : a wide variety of natural products, e. g. alkaloids, - lactames, biotin, amino sugars has been synthesized by 1, 3 - dipolar cycloaddition reaction between nitrones and alkenes
文摘:通過硝酮與烯烴化合物的1 , 3 -偶極環加成反應合成了許多天然產物,如生物堿、 -內酰胺、生物素、氨基糖等。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中,轉化大腸桿菌dh5菌株,篩選氨芐青霉素抗性菌落,提取質粒經酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原核表達載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導表達,收集菌液進行sds - page電泳、 westernblotting分析,結果表明, 3ab基因在大腸桿菌中成功表達,其表達產物為分子量33 . 5ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總蛋白量的26以上。The length of this phytase gene is1506bp interrupted once by an intron of 102bp in the 5 " part of the gene, this intron contains donor sequence - gtatgc, lariat sequence - gctgac and acceptor sequence - cag which are typically conserved sequence of the intron of fungal phytase gene. this gene encodes a peptide of 467amino acid residues with molecular weight of 51. 37kda, containing 13 potential n - glycosylation sites and a signal peptide sequence made up of 19 amino acid residues at n teminal of the peptide
核苷酸序列分析表明, pcr擴增產物中包含有完整的phya基因,該基因全長1506bp ,其中包含一段長102bp的內含子,該內含子具有真菌植酸酶基因內含子的特徵保守序列: donor序列? gtatgc , lariat序列? gctgac及acceptor序列? cag 。該基因編碼467個氨基酸,理論分子量為51 . 37kda ,其上有13個潛在的n -糖基化位點, n端19個氨基酸為信號肽序列,植酸酶活性位點序列( crvtfaqvlsrhgaryptdskgk )位於氨基酸序列的+ 71 + 93 。However, related data show that besides maillard reaction, the microbial metabolic pathway, the protein and amino acids healing processes can also produce pyrazine compounds
但據有關資料表明,除了「美拉德反應途徑」外還有「微生物代謝產物途徑、蛋白質加熱分解途徑和氨基酸類的加熱分解途徑」也可以產生吡嗪類化合物。Using a pair of degenerate primers based on the conservative region, hmg - box, of human sry gene, tow different fragments of sox gene, essox3 and essox22 were amplified from female and male eriocheir sinensis, the sequence results indicated that essox3 and essox22 shown high homology to human sox genes, and the identities to human sox genes in dna sequence and amino acid sequence are 84 % 、 85 % and 97 % 、 81 %, respectively. it might be concluded that sox gene was highly conservative in phylogenesis
二、研究論文1 、參照人sry基因hmg - box保守區的序列,設計一對兼并引物, pcr擴增了中華絨螯蟹的sox基因,並對擴增產物進行了克隆和測序。結果在雌雄個體中篩選出兩個不同的sox基因essox3和essox22 ,其dna序列和編碼的氨基酸序列與人相應sox基因的相似性分別為84 % 、 85 %和97 % 、 81 % ,顯示該基因在進化上具高度的保守性。Ghrelin regulates pituitary growth hormone ( gh ) secretion. in the study, we obtained the cdna sequences of preproghrelin and the cdna encoding the ghrelin of duck, goose and emu by using rt - pcr and 3 " - race methods. and then deduced the amino acid sequences of preproghrelin and ghrelin in duck. goose and emu. sense and antisense primers were designed on the basis of chicken ghrelin cdna sequence ( dest accession no. ab075215, 836bp ) and proventriculus cdna was performed as the template
Ab075215 , 836bp )設計引物,以鴨、鵝、鴯鶓腺胃cdna為模板,通過rt - pcr及3 - race的方法,將各個產物經過回收,再經連接轉化,克隆測序,拼接后得到鴨、鵝、鴯鶓preproghrelin基因的cdna序列及ghrelin的cdna序列,並由此推測出preproghrelin及ghrelin的氨基酸序列。O - quinones then produce the well documented brown color by reacting to form compounds with amino acids or proteins, or they self - assemble to make polymers
然後,這些無色化合物就會自我組合為聚合物,或與氨基酸或蛋白質發生反應,在蘋果組織內生成一些深色的次級產物。However, efforts to eliminate semicarbazide sources from packaging had already progressed, and manufacturers are now using sodium bicarbonate as the replacement chemical in the production of plastic seals
然而,將氨基脲清除出包裝材料的工作也已取得了進展,現在生產商們用重碳酸鹽作為氨基脲的替代化合物。Producer in china of aromatic chemicals, including derivatives of aniline, phenol, and toluidine. largest - scale product is m - aminophenol, used in dye and pharmaceutical intermediates
-主要產品有:保護氨基酸系列氨基酸衍生物系列肽類化合物系列非天然氨基酸系列及其他特殊氨基酸天然產物和醫藥中間體。The experimental method includes selecting pure complexes of histidine - containing or cysteine - containing materials, from c - and n - terminal group of these amino acids to link to a group which have color or fluorescence or ultraviolet absorption, elucidating their binding affinity, fluorescence or uv - visible spectrum properties with zinc at physiological concentration and to elucidate their structure in the solid state via infrared spectroscopy. with the help of the concerned the data, the analysis was done to prove whether it can be applied to the zinc detection, in other words, whether it can be used as a new fluorescence probe for zinc detection
本實驗首次選用在生物體內與zn ~ ( 2 + )鍵合能力很突出的物質? ?組氨酸和半胱氨酸,採用類似於多肽合成的方法,在其羧基或氨基分別嫁接上一個帶有標記的基團,生成穩定的共價鍵化合物;在此化合物中模擬生理濃度條件加入鋅離子,通過紅外圖譜、紫外圖譜或熒光圖譜的變化分析鋅離子對標記基團是否產生影響,再結合有關數據分析其是否適合檢測鋅離子,即是否可能作為新的鋅離子熒光探針。The result shows that the greater positive influence is from the dissolutions of carotene and browning reaction, next come the cembratriendid compounds and hypoglycemia compounds, while the negative influence is from indoles, toluene and some aldehydes, and the negative influence from the dissolutions of carotene and browning reaction is less
結果表明,對評吸結果影響較大的是胡蘿卜素降解產物類和棕色化反應產物類;類西柏烷類和苯丙氨酸類中性致香物質的貢獻較小;負面影響較大的成分主要集中在吲哚、甲苯及一些醛類上,類胡蘿卜素降解產物類和棕色化反應產物類中的個別成分也有一定的負面影響。Failing to install desulphurizing installations or take other measures for desulphurization in accordance with relevant regulations of the state by enterprise that discharge sulphide - bearing gas in the process of refining petroleum, producing synthetic ammonia or coal gas, cooking fuel coal and smelting non - ferrous metal
(二)排放含有硫化物氣體的石油煉制、合成氨生產、煤氣和燃煤焦化以及有色金屬冶煉的企業,不按照國家有關規定建設配套脫硫裝置或者未採取其他脫硫措施的。Gardenia blue is the blue product of the reaction between iridoids extracted from gardenia and - amino acid with the presence of proteolytic enzyme
梔子藍色素是利用梔子中提取的環烯醚萜類化合物與伯氨基酸能在水解酯作用下產生的藍色物質。Gls - amino acid derivatives products for peptide and combinatorial chemistry
吉爾生化產品氨基酸及其衍生物The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins
將帶有hwtx -基因的ppic9k經blgii線性化后,轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌,進一步用遺傳毒素g418篩選多拷貝的轉化菌株,命名為gh1 ;將gh1甲醇酵母菌用0 . 5的甲醇誘導表達,發酵上清經90飽和度的( nh _ 4 ) _ 2so _ 4沉澱, yw - 3 ( mwc03000 )的超濾膜超濾,再經cm陽離子交換, c _ ( 18 )反相hplc純化得到分子量為4kd左右的組分,其中4289 . 05的組分經質譜鑒定,氨基酸組成分析和序列測定為正確的表達產物,生物學活性表明其活性為天然毒素活性70 % ,表達量為80mg / l 。We have 4 branches, which produce active pharmaceutical ingredients, agrochemicals, peptides related substance, chiral compounds as well as fine & speciality chemicals
公司下屬4個生產基地,以原料藥&中間體、保護氨基酸&多肽縮合劑、手性化合物為主。Compared with the common cultivation, the contents of carotenoid, phenylalanine, products of browing reaction and neophytadiene were greatly increased in every treatment
並使煙葉中性致香物質總量增加,類胡蘿卜素類、苯丙氨酸類、棕色化產物、新植二烯等致香物質含量均較對照有很大提高。The results indicated that cynanchumkomarovii is sensitive to soil water from root to shoot, which apparently become slower and even stopped growing with water stress aggravation and the time of stress increase, but the root - shoot ratio increased. the soluble sugar is primary osmolyte in roots of cynanchum komarovii, which increased markedly with water stress aggravation, while soluble sugar in leaves decrease. the results showed that assimilation of cynanchum komarovii mostly distributed to root
5 、隨著土壤水分脅迫程度的加重,根系中的可溶性糖呈明顯的增加趨勢,葉中的可溶性糖則隨脅迫的加重而呈下降趨勢,說明在乾旱脅迫下牛心樸子的同化產物大部分分配于根系之中;脯氨酸( pro )在牛心樸子葉、莖、根的滲透調節中也起著一定的作用,隨土壤水分脅迫的加重,其在根、莖、葉中的積累明顯增加;而無機離子( k ~ + 、 na ~ + 、 ca ~ ( 2 + ) )在牛心樸子滲透調節中的作用似乎很小。分享友人