氨基三肽酶 的英文怎麼說
中文拼音 [ānjīsāntài]
氨基三肽酶
英文
aminotripeptidase-
Analysis of copper binding protein by sds - page, three main protein bands observed. the main bands were digested by lysyl endopeptidase and isolate different peptides by hplc. 4
Sds page分析得到3條主要蛋白帶,剪下這三條帶進行膠內賴氨酸內切酶的消化,通過高效液相色譜分離肽段,選擇性進行肽段的氨基酸序列測定。Therefore, blys, its receptor or related antagonists may find medical utility in the treatment of b cell disorders associated with autoimmunity, neoplasia, or immunodeficiency syndromes. in this study, epo signal peptide sequence and hsblys gene were linked by soe method. the fusion product was cloned into eukaryotic plasmids. pcdna3, pcdna3. 1, pefneo, respectively. meanwhile, the epo signal peptide sequence was mutated so as to form a restriction enzyme cut site : bin i. thus the recombinant plasmid can be used as secreting plasmid expressing other gene
本實驗通過3 』端互補,進行引物延伸合成epo信號肽序列:信號肽和hsblys基因採用重疊延伸拼接法形成融合基因;融合基因分別插入pcdna3 . 0 、 pcdna3 . 1 、 pefneo真核載體:引物延伸合成信號肽時,利用亮氨酸同義密碼,將信號肽基因的倒數第二個密碼突變,在重組載體上的信號肽序列之後,形成bln酶切位點,使三種載體成為分泌表達載體。There are at least three kinds of ep in petal : thiol - proteinase, metallo - proteinase, serine proteinase. they gather at the site between 43 kd and 66. 2 kd ( about 52 kd ) on the substrate gel electrophoresis. the optimum ph of thiol - proteinase, metallo - proteinase is ph 6, which are looked as acid ep, the serine proteinase has two forms, one is acid ep ( ph 6 ), and the other is alkali ( ph 10 )
花瓣中都至少有3種內肽酶:巰基蛋白酶、金屬蛋白酶、絲氨酸蛋白酶,三者聚集在底物膠電泳膠條的43kd 66 . 2kd之間的位置,約為52kd ;前兩種最適ph為6 ,是酸性內肽酶,后一種有酸( ph6 ) 、堿( ph10 )兩種存在形式,既是酸性內肽酶又是堿性內肽酶。
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