氨基末端分析 的英文怎麼說
中文拼音 [ānjīmòduānfēnxī]
氨基末端分析
英文
amino terminal analysis- 氨 : 名詞[化學] (氮和氫的化合物) ammonia; hydrogen nitride
- 末 : Ⅰ名詞1 (東西的梢;盡頭) tip; terminal; end 2 (非根本、非重要的事物) nonessentials; minor detai...
- 端 : Ⅰ名詞1 (東西的頭) end; extremity 2 (事情的開頭) beginning 3 (門類; 方面) item; point 4 (原...
- 分 : 分Ⅰ名詞1. (成分) component 2. (職責和權利的限度) what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] (料想) judge
- 析 : Ⅰ動詞1. (分開; 散開) divide; separate 2. (分析) analyse; dissect; resolve Ⅱ名詞(姓氏) a surname
- 氨基 : amido group
- 末端 : end; terminus; terminatio; terminal; tail end; extremity末端棒 terminal bar; 末端朝上 endways
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- acetolactate decarboxylase are widely found among bacterial strains but not in other groups of organisms. the enzyme has been demonstrated to be effective for removal of acetolactate and widely used in beer product. in this paper, - acetolactate decarboxylase from bacillus subtilis was purifed to homogeneity from cell extract by ammonium sulfate - fractionation, heat treatment, deae - sepharose fast flow column chromatography
本文對來源於枯草芽孢桿菌( bacillussubtilis ) 3226 - 5的-乙酰乳酸脫羧酶經硫酸銨分級沉澱、熱處理、 deae - sepharosefastflow離子交換柱層析等分離純化步驟,得到sds - paeg電泳純,通過n末端氨基酸序列分析驗證酶蛋白的純度。In this research two full - length cdnas have been cloned by a combination of rt - pcr and 3 " - is1 - race with synthesized degenerate primers from young leaves of vicia faba l., pichia methanolica high - level expression systems of the genes have been constructed, and the milligram expressed protein was purified using probond resin purification system, which may result in further identification of the function of the aba binding protein. the full - length cdna of abp370 fragment is 3449 bp long and has an open reading frame of 2304 bp encoding 768 amino acids with 876 bp long 5 ' - utr, 369 bp long 3 ' - utr and poly ( a ) tail. the full - length cdna of abp640 fragment is 1012 bp long and has an open reading frame of 780 bp encoding 260 amino acids with 88 bp long 5 ' - utr, 144 bp long 3 " - utr and poly ( a ) tail
3 - 5 - race擴增片段序列分析結果表明, abp370擴增片段的全長cdna為3449核苷酸,其中5非翻譯區為876個核苷酸, 3非翻譯區為369個核苷酸並末端帶poly ( a )尾巴,從起始密碼子atg至終止密碼子tga ,含有一個編碼768個氨基酸殘基的開放閱讀框架( 2304bp ) ; abp640擴增片段的全長cdna為1012核苷酸,其中5非翻譯區為88個核苷酸, 3非翻譯區為144個核苷酸並末端帶poly ( a )尾巴,從起始密碼子atg至終止密碼子taa ,含有一個編碼260個氨基酸殘基的開放閱讀框架( 780bp ) 。Side chain polyurethanes with azo group are characterized and confirmed by ftir, uv - vis, dsc, vpo, z - scan, polarize microscope. the results show that the end - group and the flexibility of main chain affects the growing up of liquid crystalline phase greatly. because the determinable way of detection is defective, it was not founded the liquid crystalline behavior obviously contributes to the non1inear optical thection
最後,在分析討論實驗結果的基礎上,指出側鏈型聚氨酯液晶中側鏈介晶基元的末端基團及主鏈的柔性對其液晶態的形成有重要影響;由於檢測手段的不齊備,未能觀察到熔致型聚氨酯液晶的液晶行為對其非線性光學效應的貢獻作用。So, by inhibiting the neovascularization to prevent the tumor growth and metastasis is a strategy for tumor therapy. endostatin was isolated firstly from the conditioned media of a non - metastatic murine hemagioendothelioma cell line eoma by o ' reilly in 1997. endostatin is a 20kd c - terminal fragment of collagen xvh3
1997年o ' rilly首次從小鼠內皮細胞瘤培養液中分離出內皮抑素( endostatin ) ,氨基酸序列分析顯示此蛋白是膠原c末端的氨基酸片段,分子量為20kd 。The orf1 protein of senv - h12 - 1 isolate was consisted of 675 amino acids and 92 amino - terminal amino acids less than senv - h ( ax025838 ), it had insertion in two sites also. the orf1 protein of senv - h 12 - 2 isolate was 7 amino acids less than senv - h ( ax025838 ) because of deletion. because the first start code was mutated, the orf2 protein of senv - h 12 - 2 isolate started from the second atg code, so that it was 7 amino acids less than senv - h ( ax025838 ) from its n - terminus
兩個senv - h亞型分離株的情況與已發表的博士學位論文: sen病毒分子病毒學和流行病學研究對sen琴h ( ax025838 )株分析的結果有所不同: senv - h12一1分離株的orfi僅由675個氨基酸組成,比senv - h ( axo25838 )的orfi少n一末端的92個氨基酸,此外在2個位點上還存在插入: senv - h12一2分離株在一些位點上的缺失造成o盯1蛋白比senv - h ( axo25838 )少7個氨基酸,由於第一個起始密碼子發生突變,採用位於下游的第二個起始密碼子使得o即2蛋白比senv - - h ( axo25838 )少n一末端的7個氨基酸。The amino acid analyzer could n ' t detect amino acid sequence with edman degradation, which meant apss had the blocked n - terminus
N一末端測序法不能分析apsi和」 s5氨基酸序列,估計其n一末端封閉。分享友人