氨芐基化 的英文怎麼說

中文拼音 [ānjīhuà]
氨芐基化 英文
aminobenzylation
  • 氨 : 名詞[化學] （氮和氫的化合物） ammonia; hydrogen nitride
  • 芐 : 名詞[書面語] （地黃） glutinous rehmannia
  1. Manufacturer of fine chemicals, and pharmaceutical and agrochemical intermediates, offering a small but diverse range of predominantly aromatic chemicals. located in shanghai and lanxi, china

    -生產芐胺，鄰氯苯甲酰氯，對氯苯甲酰氯， n甲基芐胺， -苯乙胺，糠胺，四甲基己二胺，延遲催化劑，聚氨酯催化劑，農藥中間體
  2. 2 benjia 4 - methyl ketone, 4 - chlorine two benjia methadone, acrylic acid resins, hydrochloric cola organism, 1023 - 1063 nitrogen zhuo methadone, phenyl acetone, reactive copper oxide, chlorobenzene oxygen ethanoic acid, ethyl ammonium chloride de base 3, 4 butadiene styrene brominated ammonium, four butadiene styrene acid hydrogen amines, amino benyi r123 for methadone, a pond amine manufacturing and marketing

    甲基二苯甲酮、 4氯二苯甲酮、丙烯酸樹脂、鹽酸可樂啶、月桂氮卓酮、苯基丙酮、活性氧化銅、對氯苯氧乙酸、芐基三乙基氯化銨、四丁基溴化銨、四丁基硫酸氫胺、二氯對氨基苯乙酮、因潢胺製造和銷售。
  3. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  4. Abstract : using sulfamie acid as catalyst, synthesis of benzyl acetate and phenylethyl acetate through esterification were studied. the yields are good with high purity

    文摘:研究了氨基磺酸催化酯化合成乙酸芐酯和乙酸苯乙酯。此工藝反應時間短、能耗低、產品純度高、產率良好、無三廢污染，優于文獻方法。
  5. 2. the resistence of transformators were selected by g418 after co - culture with agrobactrium tumeflien, which was 40 mg / l in subcultured medium and 50mg / l in differeniated medium. in the process of killing agrobactrium, cef, amp or carb were also useful and the time of inhibition was long too

    經與農桿菌共培養后轉化子的鑒定選用g418 ，在繼代篩選培養基中添加40mg l ，在分化篩選培養基中添加50mg l ；在除菌過程中，頭孢噻肟鈉、羧芐青霉素、氨芐青霉素抑菌效果較好，抑菌時間較長。
  6. First, the purified pezzis and pcr product of angiostatin are digested by ecor. i and xba i. after purifying the digested products respectively, we ligate these two kinds of dna by t4 dna ligase and construct the recombinant plasmid pezz18 - as. then transform it to the competent e. coli dh5a

    用限制性內切酶ecori與xbai對目的基因as 、表達載體pezz18行雙酶切，酶切產物純化后利用大腸桿菌t _ 4dna連接酶連接構成重組子pezz18 - as ，並轉化e . colidh5 ，經氨芐青霉素lb平板初篩后，以菌液pcr和重組子的單、雙酶切行進一步鑒定。
  7. To construct eukaryotic expression vector of mbl gene with codon 54 point mutation, the target sequence in pgem - mbld plasmid, which conains mbl cdna with codon 54 mutant allele, was amplified by pcr. after the cdna fragement and plasmids pci - neo were prepared by digestion with sma i and sal i, the fragment was inserted into sma i and sal i site in pci - neo eukaryotic expression vector, and the recombinant vector, named pci - mbl54, was obtained. the pci - mbl54, digested with restriction enzymes, was found to contain the point mutation mbl cdna by agarose gel electrophoresis analysis

    本實驗以ggc54gacmbl突變為研究對象，選用真核表達質粒pci - neo ，根據已構建好的含54位密碼子突變型mbl基因t載體的結構，設計合成新的引物， pcr擴增54位密碼突變型mbl基因，凝膠回收，雙酶切pcr產物和pci - neo質粒， t4連接酶連接，將前者克隆至後者的sma和sal位點，轉化大腸桿菌xl - 1blue ，氨芐選擇培養。
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