The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap
基因克隆到植物表達載體pbi121中，通過液
氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap
基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap
基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉
基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap
基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
Depletion of soil nitrogen frequently leads to reduced concentrations of soluble amino and amides.
土壤
氮素的減少常常導致可溶性氨
基酸和酰胺濃度的降低。
( 6 ) 4, 6 - diamino - 5 - nitrobenzofuroxan is designed to synthesis from trichlorobenzene through nitration aminatiom azidation and thermal decomposition. this compound is a new explosive. but we only got the intermediates of the first and second steps ( nitration, amination ) because the limit of time
（ 6 ）從均三氯苯出發，設計經硝化、氨化、疊
氮化和脫
氮四步合成4 ， 6 -二氨
基- 5 -硝
基苯並氧化呋咱，這是未見文獻報道的新化合物，不過由於時間關系，我們只得到氨化這一步的中間產物即3 ， 5 -二氨
基- 2 ， 4 -二硝
基氯苯，這也是個未見文獻報道的新化合物。
So the effect of eutrophication of dianchi lake of sediment ca n ' t be ignored. based on the research of modern sediemnt of dianchi lake, the thesis is concerned about some physical and chemical characteristics ( including magnetic susceptibility, ammoniac nitrogen and effective phosphprus etc. ) and thermodynamics analysis of spontaneous mineral of iron
本論文主要以滇池現代沉積物為研究對象，對滇池現代沉積物的主要物理和化學性質（包括氨
氮、有效磷、磁化率等） ，進行
基礎性地分析研究並對滇池現代沉積物鐵的自生礦物進行了熱力學分析。
1. ecological effects of long - term organophasphate pesticides contamination on soil microflora the long - term effects of organophosphate pesticides contamination on soil microflora were investigated in the present study. little difference in total counts of bacteria, actinomycetes and fungi was observed between the contaminated and the non - contaminated soil. compared with the control there were a slight decrease in total counts of free - living nitrogen - fixer and denitrifying bacteria and a significant increase in those of ammonifying and ammonia - oxidizing and nitrifying _ bacteria in the methylparathion contaminated soil
一、甲
基對硫磷長期污染對土壤微生物的生態學效應研究了有機磷農藥甲
基對硫磷長期污染對土壤微生物的影響，實驗表明：土壤細菌、放線菌、真菌總的數量影響不大；自生固
氮菌和反硝化細菌數量減少；氨化細菌、亞硝化細菌、硝化細菌的數量在污染土壤中卻有所增加；與對照土壤相比，污染土壤呼吸作用下降了29 . 93 ；氨化作用和硝化作用強度得到增強。
Fat, protein, aminophenol, trace elements in amygdalus ledebouriana schleche were determinated by chp ( 2005 edition ) and national standard methods, natural benzaldehyde and amygdalin were determinated by uv absorb method and hplc method respectively
分別用索氏抽提法、微量凱氏定
氮法測定野巴旦杏中脂肪、蛋白質，用氨
基酸分析儀測定多種氨
基酸含量，原子吸收法測定多種元素，紫外法及反相高效液相法測定天然苯甲醛及苦杏仁苷。
L - arginine : this amino acid is a precursor to nitric oxide ( no )
精氨酸：這種氨
基酸是
氮氧化物（ no ）的前身。
Asparagine can later be broken down and the nitrogen used in amino acid synthesis
天冬酰胺此後分解，
氮在氨
基酸的合成中被使用。
Silica pigment, benzene parazolone, oxalic acid catalyzer, accelerant, catalyst, deposit carbon powder, starch, paraacetaminophenetol - sulfonamide, sodiumpara - aminosalicylate ( pasna ), dalmato, p - thephalic acid, diethylbenzene - amine, titanium dioxide, acticarbon, sodium fluosilicate, fluorite, by - thiamine, silica gel powder, synthetic resin, sulfonic acid, polypropylene resin, aureomycin, pyrosodium silicate, gluchlorine acid coffee grounds, glucose, sodium sulfate, sulfide mineral, guound phosphate rock, bb, p. v. c.,
M 、觸媒、沉澱炭粉、對乙酰
氮基苯磺酰氨、對氨
基水楊酸、哆耳瑪托、對苯二酸、二乙苯銨、二氧化鈦、活性碳、氟硅酸鈉、氟石礦、副產硫銨、硅膠粉未、合成樹脂、磷酸鈣、聚丙烯樹脂、金黴素、偏硅酸鈉、糠氯酸咖啡渣、口服葡萄糠、硫酸鈉、硫化礦、磷礦粉、蘭bb 、 p . v
According to its morphological characteristics and the ability of glucose fermentation, urease production, fast blue bb salt ( dbb ) experimental 1 was identified as a rhodotorula sp
根據形態學觀察和葡萄糖發酵實驗，脲酶實驗以及重
氮基藍b實驗結果，結合《酵母菌的特徵與鑒定手冊》 ，確定y11為一株紅酵母（ rhodotorulasp ） 。
Thin sin layers and nitride - based multiquantum well ( mqw ) light emitting diode ( led ) structures with conventional single gan buffer and gan / sin double buffers were grown on sapphire substrates by metalorganic chemical vapor deposition ( mocvd )
摘要以有機金屬化學氣象沉積在藍寶石
基板上成長由單一
氮化鎵成核層與
氮化鎵/
氮化矽雙緩沖層所形成的兩種不同
氮基礎的多層量子井發光二極體結構。
Through determination of the infinite relative volatility for ethane / ethylene by means of gas stripping method, the ability of the cuprous halide amine to separate ethane / ethylene is evaluated and the factors influencing the stability of the cuprous halide amine such as the basicity of ligand, diluters, ratios are studied
摘要通過測定乙烯乙烷在含
氮基團絡合亞銅體系中的無限稀釋相對揮發度，研究了
基團堿度、配比數及稀釋劑對吸收劑選擇能力和穩定性的影響。
Test method for determination of nitrilotriacetates in detergents
洗滌劑中測定次
氮基三乙酸酯的試驗方法
Abstract : this paper has deseribed the principles of preparation and technological process about nitrogen - based sintering atmosphere by ammonium cracked and burnt. the chemical effect in sintering furnace is discussed. the effects of it on the carbon content of the iron base structure materials were studied. the superior feature and technical & economic value in saving energy, in cutting down the consumption of raw materials, in waking use of natural resources, in environmental protection, in safety, etc., have been brought to light
文摘:敘述了氨分解燃燒
氮基燒結氣氛的制備原理與流程設計；探討了它在燒結爐中的化學效應；研究了它對鐵
基結構材料碳含量與性能的影響；揭示了它用於粉末冶金鐵
基結構件燒結時，在節能、降耗、資源利用、環保、安全等方面的優勢特徵與技術經濟價值。
It is a1so referred to the nlo liquid crystal polyurthanes and the system of nlo liquid crysta1 polymer briefly. fchrmore, the possibility of combining the nlo polyurthanes and lcpu is also discussed based on azobenzene compound, whch own liquid crysta1line mesogenic unit and nonlinear opical wht togethel a series of polyurethanes with two kinds of diisocyanates were synthsized with azobenzene compounds which we prepared beforehand. the azobenzene compounds were charaterized and confirmed by ftir, " h - nmr, uv - vs, dsc, and elemeni analysis
本文第一章簡介了液晶的有關
基本概念；綜述了聚氨酯液晶的研究進展，並著重總結了聚氨酯液晶的合成方法和影響聚氨酯液晶形成液晶相的因素；介紹了非線性光學聚氨酯液晶，對非線性光學聚合物液晶體系也作了扼要的闡述，指出利用含有既是液晶
基元又是非線性光學單元的偶
氮基團的化合物合成聚氨酯液晶，實現兩者結合的可能性。
According to peak area of function group in ftir spectroscope, the different conversion time curves of [ sih ] and [ ch = ch2 ] during the reaction process were got and its reason was presented reasonably
結果表明，在反應過程中，硅
氮基與乙烯
基的濃度減少呈非線性關系，這可能是硅
氮基在反應過程中與空氣中的物質進行了反應，從而在消耗量上較乙烯
基多的緣故。
This article introduce the application of industial furnace in soldering welding technology, it is described in detailed that the principle, structure, control method and characteristics of vacuum soldering welding stove and nitrogen - base soldering welding stove
摘要介紹了工業爐在焊接工藝中的應用，引用設備實例對真空釬焊爐及氮基釬焊爐的原理、結構、控制方法及使用特點進行了詳細描述。
