活質粒 的英文怎麼說

中文拼音 [huózhílì]
活質粒 英文
biomone
  • 活 : Ⅰ動詞1 （生存; 有生命） live 2 [書面語]（救活） save （the life of a person）:活人無算 （of a goo...
  • 質 : Ⅰ名詞1 （性質; 本質） nature; character; essence 2 （質量） quality 3 （物質） matter; substance;...
  • 粒 : Ⅰ名 （小圓珠形或小碎塊形物） small particles; grain; granule; pellet Ⅱ量詞（用於粒狀物）
  1. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  2. Using enterobacter cloacae b8, the mutated strains b8b and b8f, and the recombinant clones pb and pf, we try to sequence the antagonistic - related genes of enterobacter cloacae b8 by subcloning and genome primering system. the acquired sequences were analyzed with blast program to find any homology to sequences deposited in genebank

    以廣譜拮抗菌陰溝腸桿菌b8菌株和拮抗活性缺失菌株b8b 、 b8f及從b8b和b8f二菌株克隆獲得的重組質粒pb 、 pf為基礎，對陰溝腸桿菌b8菌株拮抗相關的b和f基因片段進行序列分析。
  3. We succeeded in constructing the fusion protein plasmids of ea and ed of p - galactosidase. 2

    構建的質粒能高效表達具有較高a一互補活性的gs …蛋白、 gsthd蛋白。
  4. Further sequence analysis show that only 6 base pairs of nucleotide and 2 amino acids are different between them. the homological cry3aa gene was expressed in escherichia coli. and the expressed products which contain a fused peptide of 66 - 97 kilo - dalton was observed by means of sds - page

    生物活性測定結果表明該菌株對榆藍葉甲（ pyrrhaltaaenescens （ fairmaire ） ）和光肩星天牛等鞘翅目昆蟲具有較高的毒力，因此初步確認該菌株屬于cry3類； （ 2 ）發現該菌株中編碼毒蛋白的基因位於質粒上，並且已經成功地克隆到該基因。
  5. When both genes were co - expressed in e. coli, the activity of ppsa varied from 2. 1 - 9. 1 fold comparing to control, but the activity of tkta was relatively stable ( 3. 9 - 4. 5 fold ). whatever the two genes were expressed respectively or cooperatively, both could promote the production of dahp, the first intermediate of the common aromatic pathway, but co - expression was more effective on forming dahp and screened ppt - and ptp - as more effective. the results demonstrate that co - expression of ppsa and tkta can improve the production of dahp, and what ' s more, when multigenes co - expressed, the recombinant which has coordinated enzymes activity is optimum

    莽草酸途徑的最優化和整體調控基因csra的敲除正是上述改變的分子基礎，同時也為三種芳香族氨基酸的基因工程菌的構建打下了基礎; 7 .在國內外首次實現了共同途徑限制性底物關鍵酶ppsa刁無『及arog與分支途徑關鍵酶基因phea的串聯高效表達，所構建的重組質粒ptga ，其ppsa 、 tkta 、 arog 、 cm和pd的酶活分別比對照提高了3 、 2 、 2 ， 5 、 4 、 2 . 3倍，且其酶活比較協調一致; 8 .將ptga導入到篩選的基因敲除和基因替換菌株大腸桿菌31884 c甲b中，搖瓶發酵證實比以往所構建的基因工程菌株具有較高的phe產量和糖轉化率率，分別為0 . 448 %和22 . 4 % 。
  6. An expression vector carrying a fragment encoding the amino - terminal part of an fr - 008 type i pks module, containing a keto - synthase ( ks ) and part of an acetyl - transferase ( at ) domain was constructed for trial expression of the extremely high g + c content ( 76 % ) pks gene in plant

    為探索在植物中表達極高g + c含量的pks基因的可能性，構建了攜帶有編碼fr - 008型pks模塊氨基端部分的基因的表達質粒，包括一個酮基合酶（ ks ）和部分酰基轉移酶（ at ）活性結構域。
  7. Based on a 3. 1kb pst i fragment of genomic dna of a wild s. avermitilis, a 1. 5 kb apramycin resistance fragment was inserted into sph i site of avec gene in the 3. 1 kb fragment, then a recombinant plasmid pc05 was obtained by introducing above inactivated avec fragment into mcs region of phjl401. competent cells of et12567 were transformed by recombinant plasmid pid03 and pc05 respectively

    以含有avec基因的3 . 1kb基因組dnapsti片段為基礎，將1 . 5kb的安普黴素抗性基因片段插入到avec基因中的sphi酶切位點，再將此插入失活的avec基因片段連接到具有接合轉移功能（含有orit基因）的鏈黴菌-大腸桿菌穿梭質粒phjl401的多克隆位點區，由此得到重組質粒pc05 。
  8. The obtained recombinant - 5 - htr plasmid was tranfected into human liver cancer smmc - 7721 cells. all data suggested the expression of plncx - atr could condense cell nucleus and increase nuclear fluorescence intensity, effectively repress the telomerase activity, cell growth and cell proliferation, and induce cell apoptosis

    反義重組質粒plncx - atr轉染人肝癌smmc - 7721細胞，結果發現plncx - atr的表達有效地封閉或抑制肝癌細胞的端粒酶活性，使細胞的生長和增殖受到抑制，細胞體積變小、核緻密、核熒光強度增強，且促進其凋亡。
  9. Quantum mechanics thinks a kind of particle has a kind of corresponding field. then, there must be the universal unified field in the whole universe

    量子力學認為，一種物質粒子就有一個「場」 ，那麼整個宇宙，就有一個「宇宙統一場」 。而人是生活在宇宙之中的，所以人體生物場必定受宇宙統一場的調控和制約。
  10. 16 replacement plasmids for two different regions were obtained, but have n ' t been introduced into 10 - 22 successfully. 42 cosmids in this region were introduced into a heterogeneous host zx64, one transformant exhibited a very faint inhibit activity against fusarium oxysporum when growed on a medium with thiostrepton

    將300kb區域的柯斯質粒進行異源表達，發現有一文庫質粒5h4的轉化子在硫鏈絲菌素存在時表現出對棉花枯萎病菌微弱的抑制活性；無硫鏈絲菌素存在時，則檢測不到。
  11. Mutated plasmid was transformed into e. coli tg1 cells to produce engineered peptide, then the peptide was purified by cm sepharose ion - exchange column. in vitro bactericidal assay and drug withdrawal were used to identify the bioactivity of the engineered peptide. the planar lipid bilayer membrane was used to assay the electrophysiology of the engineered peptide. toxicity studies on mammalian cells were used to assay the toxicity of the engineered peptide

    將重組質粒轉化入大腸桿菌tgi工程菌中，生產構建的工程多膚，離子交換純化后獲得工程多膚初步純化產物，體外抗菌試驗、藥物撤離試驗檢測工程多膚的抗菌活性，在人工脂質膜上測定其形成離子通道的特性以初步研究抗菌機理， ?並觀察其對真核細胞的毒性作用。
  12. The gpa1 gene was obtained via pcr amplification and was cloned in the two - hybrid dna binding domain vector pgbkt7 the combinant plasmid was designated as pgbkt7 - ga. - galactosidase assay indicated that got did not have the property of self - activation. after pgbkt7 - ga was transformed to yeast pj69 - 4a, we transformed arabidopsis vegetative tissue two - hybrid cdna library plasmids to yeast pj69 - 4a containing pgbkt7 - ga

    通過pcr擴增得到gpa1基因並將其克隆到雙雜交dna結合域載體pgbkt7中，得到的重組質粒命名為pgbkt7 - g ， ?半乳糖苷酶活性鑒定表明g不具有自激活特性，將其轉化到酵母菌pj69 - 4a中，再以此為受體菌轉化擬南芥綠色營養組織cdna文庫質粒。
  13. With the precondition that invitro tissue could survive after implantation, the study on transferring gus gene into arabidopsis callus was done. and we got the transformants. transformation efficiency was up to 2 %

    在活體組織注入后可以存活的前提下，通過對離子束介導質粒dna導入擬南芥愈傷組織的遺傳體系的研究，得到了gus基因轉入擬南芥愈傷組織的轉化植株，轉化效率達2 。
  14. In this thesis the lbsp identification medium screening techniques was used. only after one cycle, large quantities no - function recombination plamid and enzyme activity increasing 20 times recombination plasmid were obtained

    本研究用lbsp鑒別培養基直接篩選技術，僅一個循環就獲得了大量無酶活的重組質粒和酶活提高了20倍的突變基因。
  15. Lymphotoxin ( lt ) is a kind of pleiotropic lymphocyte - secreted cytokine which mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. in order to increase the antitumor activity of lymphotoxin and reduce its side effects, the recombinant plasmid pet36b - lt 27 was constructed to express soluble fusion protein cbd - lt 27. the active form of lt 27 could be collected directly with several simple steps by three kind of components on the expressed fusion protein

    本研究通過構建表達n端缺失27個氨基酸的淋巴毒素融合蛋白的重組質粒，在大腸桿菌中實現融合蛋白的可溶及分泌表達，同時利用表達載體上的幾種特殊序列經簡單的分離純化步驟直接獲得大量的有生物活性的淋巴毒素缺失體lt 27 ，為尋找一種高抗腫瘤活性、低臨床毒副作用的生物抗癌藥物進行了有效的探索。
  16. The research showed that their interactions in yeast two - hybrid system are still steady when the vector was exchanged, in contrast, the interaction was disappear when the reading frame of each positive had been changed. the four positives were subcloned into suicide plasmid so that gene mutant strains could be constructed via conjugation and homologous recombination

    為了進一步驗證這些克隆的編碼產物與nifa蛋白的相互作用，將它們與nifa基因互換載體后共轉化酵母，仍然可以激活三個報告基因的表達，而陽性克隆移碼表達的重組質粒和pgbd - nifa的共轉化物則不能在選擇性平板上生長。
  17. The main mechanisms of earthworms ' resistance to metal pollutants are also elaborated : ( 1 ) its lipid antioxidative enzyme system helps relieve the stress of oxidation ; ( 2 ) compartment and immobilization of metals ; ( 3 ) process of chelating and detoxicification ; ( 4 ) lysosome and cellular plasmid are activated to restrain activity of heavy metals

    並闡述了蚯蚓對重金屬的主要耐性機制： （ 1 ）脂質過氧化保護酶系統緩解氧化脅迫； （ 2 ）分隔、固定作用； （ 3 ）螯合解毒作用； （ 4 ）溶酶體和細胞質粒抑制重金屬活性。
  18. Furthermore, we discussed the influence of immune - sensibilization before induction to the activity of t - cell vaccine, compared the immune - sensibilization difference between hcv - adenovirus vectors and plasmid vectors

    此外，我們還探討了誘導前免疫致敏對t細胞疫苗活性的影響，並比較了hcv腺病毒載體和質粒載體在此免疫致敏功能方面的差異。
  19. Objective, clone tissue - type plasminogen activator ( t - pa ) gene and construct a new kind of recombinant vector containing human tissue - type plasminogon activator ( t - pa ) cnda neither cytotoxiaty nor actovating prot - oncogenes

    目的：克隆組織纖溶酶原激活物（ t - pa ）基因並構建一種無細胞毒性、不激活原癌基因的真核表達的pcdna3 . 1 （ + ） / t - pa質粒載體。
  20. A 1. 5 kb apramycin resistance fragment was inserted into nru i site of aved gene and the inactivated aved gene fragment was then introduced into mcs region of phjl401 - an e. coli / streptomyces shuttle vector with conjugation function ( containing orit gene ). as a result of above procedures, a recombinant plasmid pid03 was obtained

    將1 . 5kb的安普黴素抗性基因片段插入到aved基因中的nrui酶切位點，再將此滅活的aved基因片段插入到具有接合轉移功能（含有orit基因）的鏈黴菌?大腸桿菌穿梭質粒phjl401的多克隆位點區，由此得到重組質粒pid03 。
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