活酵母培養 的英文怎麼說
中文拼音 [huójiàomǔpéiyǎng]
活酵母培養
英文
live yeast culture- 活 : Ⅰ動詞1 (生存; 有生命) live 2 [書面語](救活) save (the life of a person):活人無算 (of a goo...
- 酵 : 動詞(發酵) ferment; leaven
- 母 : Ⅰ名詞1 (母親) mother 2 (泛指女性長輩) one s elderly female relatives 3 (配套的兩件東西里的凹...
- 培 : 動詞1. (在根基部分堆上土) bank up with earth; earth up 2. (有目的地使成長、壯大) cultivate; foster; train
- 養 : Ⅰ動詞1 (供養) support; provide for 2 (飼養; 培植) raise; keep; grow 3 (生育) give birth to ...
- 酵母 : yeast酵母浸液[提取液] yeast extract; 酵母聚糖 zymosan; 酵母片 aluzyme; 酵母細胞[植物] yeast plant; yeast cell
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Effect of yeast culture on egg - chickling ' s production performance
高活性酵母培養物對蛋雛雞生產性能的影響The deleted mutant pap gene was also cloned into yeast secreted expression ppic9k vector to form ppic9k ~ 3, then the vector was transferred into pachia pastoris gs115 strain. the specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50 - 60 u g per millilitre measured by uv - absorbed methods in the supernatant of the medium via high density fermentation. sds - page results showed that there was one protein band in the gel which molecular weight was about 34ku
將缺失型pap基因克隆于酵母分泌型表達載體ppicgk構成重組載體,然後導入畢赤酵母( p8chianastoris )菌株gslls細胞中,在甲醇的誘導下,經過酵母高密度發酵進行pap的表達,經sds page分析,結果表明,在培養基上清液中含有一明顯的特異性蛋臼條帶,大小為34ku ,經western blotting分析,該蛋白與法國pap抗血清有特異性反應,體外活性檢測表明該蛋白對tmv的侵染性具有高度的抑制性,說明該pap基因在畢赤酵母gs中也得到了正確表達。Effect of yeast cultures on fibrolytic bacterial population and activities of fiber hydrolytic enzymes in the rumen
添加不同酵母培養物對瘤胃纖維分解菌群和纖維素酶活的影響It produced strong antifungal bioactive substance on medium containing 4 % of starch, 0. 5 % of yeast extract and 75 % of stale seawater cultured at 28 for 96h
海洋微生物菌株041381利用澱粉培養基(澱粉4 ,酵母膏0 . 5 ,陳海水75 ) ,在28下靜置發酵培養96h產抗真菌活性物質。The mutant pel - d92l was expressed in pichia pastoris gs115, sds - page detection showed that the expression product pel - d92l - gs is different from pel - gs, and its " yield decreased dramatically, the themostability of pel - d92l - gs is also different from the pel - gs, but their optimum temperatures are same. 3. directed evolution of pel through random mutagenesis mutagenesis pcr carried out in error - prone conditions was used on the vector psk - pel, using the oligos " beginning " and " end ", homologous to the 5 ' and to the 3 " ends of the gene of pel respectively
三、 pel基因的隨機誘變用易錯pcr方法對pel基因進行隨機誘變, pcr產物與ppic3 . 5k連接,轉化大腸桿菌,獲得的混合質粒電轉化畢赤酵母gs115 , omm平板篩選適于低溫或對熱穩定的重組子,篩選獲得一株最活反應溫度、熱穩定性、發酵酶活均有提高的突變體pel - ep5 - gs ,其最活反應溫度為45 ,比野生型高出5 ; 40處理30min殘留活性為56 ,大大高於野生型的6 ;初始ph7 . 3528條件下培養72h ,發酵上清酶活為325u / ml 。分享友人