流式細胞檢 的英文怎麼說
中文拼音 [liúshìxìbāojiǎn]
流式細胞檢
英文
wb- 流 : Ⅰ動1 (液體移動; 流動) flow 2 (移動不定) drift; move; wander 3 (流傳; 傳播) spread 4 (向壞...
- 式 : 名詞1 (樣式) type; style 2 (格式) pattern; form 3 (儀式; 典禮) ceremony; ritual 4 (自然科...
- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 胞 : Ⅰ名詞1 (胞衣) afterbirth2 (同一個國家或民族的人) fellow countryman; compatriot Ⅱ形容詞(同胞...
- 檢 : Ⅰ動詞1 (查) check up; inspect; examine 2 (約束; 檢點) restrain oneself; be careful in one s c...
- 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
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Detect the glycoprotein of platelet with chd by fcm
流式細胞術檢測冠心病患者血小板表面糖蛋白的變化Using flow cytometry to detect peripheral blood eosinophils and their related molecules
流式細胞術在外周血嗜酸性粒細胞及其相關分子檢測中的應用Use pi staining of fixed cells and facs analysis to determine type of u 2 - os cell death induced by bfgf
採用固定后細胞pi染色流式細胞術檢測bfgf誘導u2 os細胞死亡的形式。Facs results showed that umbilical cord blood mscs did not express antigens cd34, cdlla and cdllb, and expressed cd29 and cd71, which was identical to that of human bone marrow - derived mscs
用流式細胞儀檢測msc的表面標志結果顯示,臍帶血msc不表達cd34 、 cd11a和cd11b ,強表達cd29 ,弱表達cd71 ,與骨髓msc表面抗原特性一致。Flow cytometry measurements were done to detect the changing of fluorescent signal of the reporter strain and give expression situation to ybt indirectly. 7 eaggec hpi - positive strains revealed an enhanced fluorescence signal but 1 eaggec hpi - negative did not so
3n ) ,將待測eaggec菌株的培養上清加入該報告菌株培養物中,用流式細胞術facs方法檢測報告菌株熒光強度的變化情況,間接反映ybt的表達與否。Mdr1 express product p - glycoprotein was detected by immunocytochemical method and flowcytometry. the cytotoxicity and multidrug resistance reversion effect of tea polyphenol was examined by mtt assay in mcf - 7 and mcf - 7 adr carcinoma cell lines, and compared with pgp inhibitor quinidine. the pgp expression of mcf - 7 adr was strongly positive, the positive rate was 15 % ; the pgp expression of mcf - 7 was negative, the positive rate was 1. 8 %. ic50 of tea polyphenol to mcf - 7 and mcf - 7 adr is 115. 2g ml and 207. 6g ml respectively. ic50 of quinidine to mcf - 7 and mcf - 7 adr is 129. 8mol l 42. 1g ml and 94. 1mol l 30. 5g ml respectively. tea polyphenol and quinidine changed little toxicity of adriamycin to mcf - 7, but tea polyphenol and quinidine improved the sensitivity of mcf - 7adr to adriamycin significantly. immunocytochemistry and flow cytometry can detect p - glycoprotein expression level qualitatively and quantitatively. tea polyphenol is not only an anti - tumor agent, but also a multidrug resistant modulator similar as quinidine. tea polyphenol is advantageous for its little toxicity in tumor treatment
用免疫組化法和流式細胞儀對腫瘤細胞系mcf - 7和mcf - 7 adr的p -糖蛋白表達水平進行定性定量研究。用噻唑藍比色法mtt研究茶多酚的細胞毒性及其對耐藥性的逆轉作用,並與pgp抑制劑奎尼定進行了比較。免疫組化法檢測p -糖蛋白表達水平, mcf - 7 adr呈強陽性,而mcf - 7呈陰性流式細胞儀定量檢測結果mcf - 7 adr細胞系細胞陽性率為15 % , mcf - 7細胞系細胞陽性率為1 . 8 % 。Smyth mj, krasovskis e, sutton vr, et al. the drug efflux pro tein, p - glyoprotein, additiionally protects drug - resistant tumor cells from multiple forms of caspase - dependenl apoptosis [ j ]. proc natl acad sci usa, 1998, 95 : 7024
闞兵,羅鋒,雷松,等.用流式細胞術檢測腫瘤組織細胞多藥耐藥基因表達產物[ j ] .華西醫科大學學報, 1998 , 29 ( 3 ) : 269Now it has been found the recruitment and homing of circulating endothelial cells ( cecs ) or their progenitors ( ceps ) released from the bone marrow is very important in tumor angiogenesis. we detected the cecs and ceps in 64 pb ( peripheral blood ) of tumor patients. we found the amount of cecs and ceps is higher in tumor patients than normal controls
本項目應用流式細胞法對64例腫瘤患者及15例正常人,外周血中的循環血管內皮細胞( circulatingendothelialcell , cec )以及骨髓來源的循環血管內皮前體細胞( circulalingendothelialprecursor , cep )進行了檢測,證實了外周血中cec / cep的存在,發現腫瘤患者cec / cep含量高於正常對照組,提示腫瘤患者體內存在血管內皮及其前體細胞的動員。In our report, we selected trehalose to substitute sucrose in the cryoprotectant. the process of vitrification is under the same preferable condition. the survival - rate of the recovered cells was tested by fda - pi and cd34 + cell - count
凍融后的細胞以fda - pi雙熒光染色、流式細胞儀cd34 +計數等檢測手段,與同樣條件的60蔗糖保護劑處理的細胞相比較。After adding culture mediem of stably transfected jurkat cells to hepatocarcinoma cells, the binding specificity of the scfvs with hbsag was further confirmed by observation by fluorescence microscope, indirect immunofluorescence and flow cytometer analysis. prokaryotic expression plasmids ptat - ha - scfvs were successfully constructed
建系細胞培養上清與肝癌細胞作用后,經熒光顯微鏡觀察、間接免疫熒光及流式細胞儀檢測進一步確定表達的scfv融合蛋白具有與hbsag特異性結合的活性。Et and nos immunochemical staining and the analyzing the apoptosis in acute myocardial ischemia may be a sensitive method to diagnose the sudden death caused by ami in forensic medicine
故此,通過流式細胞儀檢測心肌的凋亡指數並結合超敏sp法對心肌進行et 、 inos和enos聯合免疫組化染色,可以輔助診斷ami所致的碎死。Methods : 1 ) 12h after irradiation, the cell cycle of nih3t3 cells was determined by flow of cytometry and the ratio of alternations in p16 gene exon - 2 was evaluated through pcr - sscp. 2 ) the content of mda, the activities of the sod and gsh - px in the supernatant of nih3t3 cells and the cells were measured by detecting kits immediately after irradiation. 3 ) the level of matrix metalloproteinase - 2 ( mmp - 2 ) in hela cells was detected by western - blotting and dot - blotting 2h after irradiation
具體方法為: ( 1 )照射后12h ,收集nih3t3細胞,用流式細胞儀檢測各組細胞的細胞周期, pcr - sscp檢測抑癌基因p16的變化; ( 2 ) nih3t3細胞照射后立即收集細胞和細胞上清,用試劑盒測量mda含量和sod 、 gsh - px的活性並觀察其變化; ( 3 ) western免疫印跡和點雜交法檢測照射2h后的各組hela細胞中基質金屬蛋白酶- 2 ( mmp - 2 )的表達變化。( 4 ) cell cycle distribution by flow cytometry
( 4 )流式細胞儀檢測細胞周期分佈。Regulation of toll - like receptor 4 expression on murine dendritic cells mediated by cd137 signal
方法以流式細胞術檢測不同劑量Apoptotic peak. agarose gel electrophoresis showed that 185 - base pair ladder characteristic of the dna degradation that occurs in apoptotic cells induced by a
電泳出現階梯狀條帶流式細胞儀檢測出現典型的凋亡峰,提示It was finded by using flow cytometry that the apoptosis rate of porcine ear skin fibroblasts cultured under a variety of cell cycle - arresting treatments was quite low
通過流式細胞儀檢測,發現不同處理的豬耳皮膚成纖維細胞的凋亡率均較低。In this work, effects of sfamnpv on mitochondrial and er in sl - 1 cells were studied. the main results were as follows : effect of sfamnpv ( moi = 6 ) on the function of mitochondrial in sl - 1 cells was investigated by mtt assay and membrane potential was assayed by both flow cytometry and confocal laser scanning microscope
完成的工作包括以下幾個方面: ( 1 )細胞內線粒體的變化通過mtt法研究病毒感染對細胞線粒體功能的影響,同時用流式細胞儀和共聚焦激光顯微鏡研究線粒體膜電位( m )的變化, western檢測2種凋亡調控蛋白cytoc和bcl - 2蛋白的變化。Light microtechnique and sa - galactosidase method was used to study the effects of super - high - concentration of glucose on the senescence of human diploid fibroblast 2bs cells, ros and the membrane potential of mitochondria during this process were measured. our results showed that 200 mmol l of glucose inhibited the growth of 2bs cells, led to the changes of reactive oxygen species and decrease of mitochondrial membrane potential, and caused senescence of 2bs cells rapidly. it supports the hypothesis of oxidative damage of senescence. moreover it is a better system for the study of the effects of ros during the process of replicative senescence
利用光學顯微鏡觀察和酸性-半乳糖苷酶染色技術研究了高濃度葡萄糖對人二倍體成纖維細胞2bs細胞衰老進程的影響,並用流式細胞儀檢測了此過程中活性氧和線粒體膜電位差的變化。結果表明: 200 mmol l的葡萄糖對2bs細胞有生長抑制作用,能引起活性氧含量的變化,導致線粒體膜電位差顯著下降,並誘導了細胞的衰老。這為氧化損傷假說提供了新的證據,並為研究活性氧和復制衰老之間的關系提供了較好的體系。One measure of malignancy is aneuploidy by flow cytometry
惡性腫瘤的檢測方法之一是通過流式細胞計顯示異倍性。This paper is the working results of flow cytometric measurements of ultraphytoplankton in east china sea and yellow sea, which including the works of two cruises in autumn 2000 and spring 2001 and two - week time series observation in summer 2001. in addition, in situ enrichment experiments were designed at e2 station of spring cruise
本文主要內容為流式細胞計在中國東、黃海對超微型浮游植物的檢測結果報導,所做工作包括: 2000年秋季及2001年春季的兩個航次、 2001年夏季( 7月)在長江口區的定點長時觀測,此外,還在春季航次e2站進行了現場營養鹽加富培養實驗。分享友人