流式胞 的英文怎麼說
中文拼音 [liúshìbāo]
流式胞
英文
flow cytometry-
Detect the glycoprotein of platelet with chd by fcm
流式細胞術檢測冠心病患者血小板表面糖蛋白的變化Application of flow cytometry in sperm chromatin structure assay
流式細胞術在精子染色質結構分析中的應用In the first trial, combination of enzymatic digestion was used to prepare suspensions of spermatogenic cells from adult mouse testis, and then a modified discontinuous percoll gradient centrifugation method ( 15 %, 22 %, 30 %, 40 %, 50 %, 60 % ) was introduced to isolate spermatids from the cellular suspensions. the content of spermatids in each isolated fraction by percoll method was determined by morphology ( wright - giemsa stain ) and flow cytometry analysis, and the viability of spermatogenic cells was assessed by using eosin y exclusion test
在第一部分試驗中，首先利用連續3次組合酶消化成年小鼠睪丸制備睪丸細胞懸液，然後經6層非連續percoll梯度離心法（ 15 、 22 、 30 、 40 、 50和60 ）分離，通過形態學和流式細胞術鑒定南京醫科大學碩士學位論文各個percoll組分中精子細胞的含量，並以伊紅y排斥試驗測定細胞的存活率。Using flow cytometry to detect peripheral blood eosinophils and their related molecules
流式細胞術在外周血嗜酸性粒細胞及其相關分子檢測中的應用Use pi staining of fixed cells and facs analysis to determine type of u 2 - os cell death induced by bfgf
採用固定后細胞pi染色流式細胞術檢測bfgf誘導u2 os細胞死亡的形式。There appears obvious apoptotic peak on dna content curve of facs analysis. and as the time of bfgf treatment increases, percent of apoptotic cells increases. 3
在流式細胞術dna含量曲線上顯示出明顯的亞二倍體峰（凋亡細胞峰入並且隨著bfgf作用時間的延長， u2 os細胞凋亡百分比逐漸增加。Facs results showed that umbilical cord blood mscs did not express antigens cd34, cdlla and cdllb, and expressed cd29 and cd71, which was identical to that of human bone marrow - derived mscs
用流式細胞儀檢測msc的表面標志結果顯示，臍帶血msc不表達cd34 、 cd11a和cd11b ，強表達cd29 ，弱表達cd71 ，與骨髓msc表面抗原特性一致。Flow cytometry measurements were done to detect the changing of fluorescent signal of the reporter strain and give expression situation to ybt indirectly. 7 eaggec hpi - positive strains revealed an enhanced fluorescence signal but 1 eaggec hpi - negative did not so
3n ） ，將待測eaggec菌株的培養上清加入該報告菌株培養物中，用流式細胞術facs方法檢測報告菌株熒光強度的變化情況，間接反映ybt的表達與否。Thus, in order to investigate the developmental pathways not only involved in the regulation of growth and patterning, but also in the determination of cell lineages and differentiation, we utilized the fluorescent immunohistochemical methods, flow cytometry analysis sorting ( facs ) and molecular methods to investigate the developmental law of mammary gland at the different developmental stages, distribution of the stem cells in mammary gland, the methods of isolation, culture and evaluation for the stem cells, the multipotent abilities in vivo and in vitro, and the efficient cultural system for stem cells enriched in vitro. the results showed below : 1
我們以小鼠為模型，運用組織化學、免疫熒光組織（細胞）化學、流式細胞儀分選方法（ facs ）以及分子生物學手段，研究了小鼠乳腺的發育規律：小鼠乳腺組織中類乳腺幹細胞：小鼠乳腺細胞的分離、培養以及類乳腺幹細胞的鑒定；小鼠類乳腺幹細胞分化的潛能；小鼠乳腺類腺體體外短期培養富集類乳腺幹細胞體系的優化等。研究結果表明： 1Effect of lomerizine on multidrug resistance in human leukemic cell line k562 a
水平;以流式細胞術測定兩種細胞系內柔紅黴素Immunophenotyping of peripheral blood leukemic cells by multi - parameter cytometry
多參數流式細胞術對外周血白血病免疫分型的研究Study of the relationship between cell size, chlorophyll content of aegiceras corniculatum mesophyll and seawater salinity by fcm
用流式細胞儀探討桐花樹葉肉細胞大小和葉綠素含量與海水鹽度的關系We used fission yeast schizosaccharomyces pombe ( s. pombe ), an unicellular eukaryotic organism, as research material. electroporation was adopted to load ca2 + fluorescent indicator into yeast cell and under the laser scanning confocal microscopy ( lscm ), we observed cytosolic ca2 + distribution and relative content as well as fluorescence intensity of gfp - cam in different phases of cell cycle of yeast cell. flow cytometry provided a way of determining the relative dna content of populations of fission yeast
本文以單細胞的真核模式生物裂殖酵母（ schizosaccharomycespombe ）為研究材料，通過激光掃描共聚焦顯微鏡觀察酵母細胞胞質內游離ca ~ （ 2 + ）的分佈及相對濃度，以及不同周期時相細胞中gfp - cam的熒光強度變化，並採用細胞流式法對酵母細胞的相對dna含量進行測定以確定細胞所處周期時相。Mdr1 express product p - glycoprotein was detected by immunocytochemical method and flowcytometry. the cytotoxicity and multidrug resistance reversion effect of tea polyphenol was examined by mtt assay in mcf - 7 and mcf - 7 adr carcinoma cell lines, and compared with pgp inhibitor quinidine. the pgp expression of mcf - 7 adr was strongly positive, the positive rate was 15 % ; the pgp expression of mcf - 7 was negative, the positive rate was 1. 8 %. ic50 of tea polyphenol to mcf - 7 and mcf - 7 adr is 115. 2g ml and 207. 6g ml respectively. ic50 of quinidine to mcf - 7 and mcf - 7 adr is 129. 8mol l 42. 1g ml and 94. 1mol l 30. 5g ml respectively. tea polyphenol and quinidine changed little toxicity of adriamycin to mcf - 7, but tea polyphenol and quinidine improved the sensitivity of mcf - 7adr to adriamycin significantly. immunocytochemistry and flow cytometry can detect p - glycoprotein expression level qualitatively and quantitatively. tea polyphenol is not only an anti - tumor agent, but also a multidrug resistant modulator similar as quinidine. tea polyphenol is advantageous for its little toxicity in tumor treatment
用免疫組化法和流式細胞儀對腫瘤細胞系mcf - 7和mcf - 7 adr的p -糖蛋白表達水平進行定性定量研究。用噻唑藍比色法mtt研究茶多酚的細胞毒性及其對耐藥性的逆轉作用，並與pgp抑制劑奎尼定進行了比較。免疫組化法檢測p -糖蛋白表達水平， mcf - 7 adr呈強陽性，而mcf - 7呈陰性流式細胞儀定量檢測結果mcf - 7 adr細胞系細胞陽性率為15 % ， mcf - 7細胞系細胞陽性率為1 . 8 % 。Smyth mj, krasovskis e, sutton vr, et al. the drug efflux pro tein, p - glyoprotein, additiionally protects drug - resistant tumor cells from multiple forms of caspase - dependenl apoptosis [ j ]. proc natl acad sci usa, 1998, 95 : 7024
闞兵，羅鋒，雷松，等.用流式細胞術檢測腫瘤組織細胞多藥耐藥基因表達產物[ j ] .華西醫科大學學報， 1998 ， 29 （ 3 ） : 269Now it has been found the recruitment and homing of circulating endothelial cells ( cecs ) or their progenitors ( ceps ) released from the bone marrow is very important in tumor angiogenesis. we detected the cecs and ceps in 64 pb ( peripheral blood ) of tumor patients. we found the amount of cecs and ceps is higher in tumor patients than normal controls
本項目應用流式細胞法對64例腫瘤患者及15例正常人，外周血中的循環血管內皮細胞（ circulatingendothelialcell ， cec ）以及骨髓來源的循環血管內皮前體細胞（ circulalingendothelialprecursor ， cep ）進行了檢測，證實了外周血中cec / cep的存在，發現腫瘤患者cec / cep含量高於正常對照組，提示腫瘤患者體內存在血管內皮及其前體細胞的動員。For small scale systems such as convective cells or thunderstorms with dimensions under 10 km that affect hong kong every summer, the current model resolution is still not quite fine enough fig. 6
對于夏季影響香港，范圍不及10里的對流細胞及雷暴這等細小系統而言，目前的模式解析度仍覺不足圖表6 。For small scale systems such as convective cells or thunderstorms with dimensions under 10 km that affect hong kong every summer, the current model resolution is still not quite fine enough ( fig. 6 )
對于夏季影響香港，范圍不及10里的對流細胞及雷暴這等細小系統而言，目前的模式解析度仍覺不足（圖表6 ） 。In our report, we selected trehalose to substitute sucrose in the cryoprotectant. the process of vitrification is under the same preferable condition. the survival - rate of the recovered cells was tested by fda - pi and cd34 + cell - count
凍融后的細胞以fda - pi雙熒光染色、流式細胞儀cd34 +計數等檢測手段，與同樣條件的60蔗糖保護劑處理的細胞相比較。The principle of flow cytometer and its clinical application
流式細胞儀工作原理及臨床應用分享友人