浸液培養基 的英文怎麼說
中文拼音 [jìnyèpéiyǎngjī]
浸液培養基
英文
infusion medium-
The genotype is a main factor in the genetic transformation via agrobacteriwn - mediated. the results of orthogonal experiments of the factors which affect mainly the transformation illuminates that the erniuxin genotype + bacterium 15 x + explant + dip - dye is the best one. the cocultivation time is 28 days. the experiment shows that : the erniuxin cotyledon regeneration frequency is very high, and that the dongnong901 hypocotyl regeneration frequency is very high
確立了農桿菌介導轉化過程中基因型是影響轉化率的主要因素。通過極差分析，基因型為「二牛心」 ，菌液濃度15倍的，外植體為子葉，感染方法為浸泡法是最佳的組合。農桿菌與外植體共培養的時間為28h 。In case of environmental samples ( e. g. swabs or sponges ) volumes may need to be adjusted to ensure that the whole sample is covered by pre - enrichment broth )
如果是環境樣（棉簽或海綿）可以調整培養基體積以確保整個樣品被預富增液浸泡。Calli were induced by culturing hypocotyls of pepper in the ms medium supplemented with 2. 0mg / l 6 - ba and l. omg / l naa. the diploid wound callus was treated by adding colchicines into the medium and by immersing into colchicines solution for different concentrations and periods
通過將不同濃度的秋水仙堿加入培養基和不同濃度的秋水仙堿溶液浸泡兩種方法處理愈傷組織，從而篩選出最佳的誘導方法和最佳的秋水仙堿濃度和處理時間。6. transformation system of mustard a serials of kanamycin concentration was added to optimum medium to test the explants resistance capacity of two kinds of mustard. the transformation procedures described were derived from numerous regeneration and trasformation designed to test factors that might affect shoot regeneration, which including length of co - cultivation. those producing the best result parameters were described as below : after the mustard explants were precultured on regeneration medium for 2 days. they were inoculated with agrobacterium for 20 minutes. inoculated explants were co - cultivated for 4 days and in shadow at first 2 days. then transferred to the same medium plus 30 mg / l kanamycin and 500mg / l garb. all of them were transferred to fresh medium every 2 weeks. the kan - resistant plants were regenerated
芥菜外植體高頻遺傳轉化體系的建立在最適培養基上試驗了兩類芥菜的三種外植體對卡那黴素的敏感性、預培養天數、浸菌時間等因素的影響，建立了芥菜高頻轉基因再生體系：取生長4天的芥菜子葉、下胚軸和25天的葉片在分化培養基上（ ms + ba3 . 0mg / l + naa0 . 1mg / l ）預培養2 - 3天後，投入農桿菌菌液中浸染20分鐘，在分化培養基上暗培養2天，正常條件下培養2天後，轉入抗性培養基（ ms ba3The eg cell culture media consisting of dmem medium supplemented with fbs, chicken serum, beta - mercaptoethanol, l - glutamine. hepes, chicken embryonic extract and cytokines etc. after 24 hours culture, the isolated pgcs were selectively attached on the gonadal stromall cells in the plates
加入新鮮的eg細胞培養液（含dmem 、胎牛血清、雞血清、 -巰基乙醇、 l -谷氨酰胺、 hepes 、雞胚浸出液以及細胞因子等成分）培養24小時以後， pgcs開始部分貼附於共培養的生殖原基細胞上。Colchicine concentrations were four levels of 100, 500, 1000 and 2000 mg / l, and treatment periods were of 1, 2, 4 and 6 days for the former and 24, 48 and 72h for the latter. the results showed that both the two methods can induce polyploid cells, but the former was better than the latter in the induction rate and operating
經染色體數目鑒定表明，這兩種方法都能有效的誘導四倍體細胞（ 2n = 4x = 48 ）的產生，而二倍體染色體數為2n = 2x = 24 ，但是加入培養基法無論在誘導率上還是在操作上都優于液體浸泡法。Our experiment indicates : ( 1 ) the optimal concentration of kanamycin for screening torenia fournier regenerated buds was 400 mg / l. the ideal transformation was obtained in the following conditions : the leaf discs were dipped in agrobacterium suspension that od600 was 0. 1 for 10 ~ 20 min ; subsequently cocultivated on the ms solid coculture medium containing 20umol / l acetosyringon for 7 to 8 d at 23, and the induction ratio of regenerated buds was 27. 2 %
研究結果表明： （ 1 ）篩選藍豬耳轉化芽的最適卡那黴素濃度為400mg l 。 od _ （ 600 ）為0 . 1的菌液濃度菌液浸染葉盤10 20min ，固體共培養基（含20 mol l乙酰丁香酮）上23共培養7 8d可獲得理想的轉化效率，轉化芽誘導率為27 . 2 ； 16h光照， 8h黑暗是較理想的共培養光周期；莖段是較好的轉化受體。4. transformation and selection of plants. leaf explants had been pre - cultured for 2 days, then immersed in agroba - - cterium suspension for 5 ~ 6minutes
轉化體篩選及植株再生將預培養2天的外植體與農桿菌菌液浸染5 6分鐘后，共培養2 3天，然後轉化到含km75m旮的選擇培養基上進行分化篩選，再轉入k 。分享友人