液化腸桿菌 的英文怎麼說

中文拼音 [yèhuàchánggǎnjūn]
液化腸桿菌 英文
enterobacter liquefaciens
  • 液 : 名詞（液體） liquid; fluid; juice
  • 腸 : 名詞1. （消化器官的一部分, 通稱腸子） intestines 2. （用腸衣塞肉、魚等製成的食品） sausage 3. （感情; 情緒; 情感） heart
  • 桿 : 桿名詞（桿子） pole; staff
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 液化 : [化學] liquefaction; deliquesce; liquefying; liquidation液化劑 liquefier; fluidifiant; liquefacie...
  • 桿菌 : [微生物學] bacillus
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. Israelensis recombinants, which contained recombinants plasmid pmt4 and pmt9 respectively, were obtained by electroporation. the bioassay results showed that the recombinants b - pmt9 and b - pmt4 had toxicities both to resistant and susceptible c. quinqnefasciatus larvae during vegetative growth stage, having the lc ? o values similar to that of. fi. sssii - 1. however, the toxic levels of the final sporulated cultures of recombinants b - pmt4 and b - pmt9 differed, with a lcso value of 2 49mg / ml for b - pmt9 and little toxicity for b - pmt4 by using the plasmid pmt9, m txl gene from b. sphaericus was ligated with p20 and cytjaa gene, giving recombinant plasmid pmpx2

    含有pmt9和pmt4的大腸桿菌轉化子能表達產生mtx1毒素，發酵液對敏感和抗性致倦庫蚊幼蟲具有中度毒殺作用；含有pmt9和pmt4的蘇雲金芽孢桿菌轉化子b - pmt9和b - pmt4在營養體生長階段對敏感蚊幼和抗性幼蟲也具有毒性，毒力與野生型b . sss - 1相當，而不同轉化子在芽孢形成期的毒力因插入的mtx1基因轉錄方向不同而表現出差異，其中b - pmt4對目標蚊幼毒力極低（ lc _ （ 50 ） 10mg ml ） ，而b - pmt9對蚊幼蟲具有毒性（ lc _ （ 50 ） = 2 . 49mg ml ） 。
  3. The total rna was purified from the germ in the liquid by the guanidine isothiocyantehod method, then the total rna digested by dnase that had not rnase was used for rt - pcr. i change the magnesium ion dencity in the pcr system in order to optimize the pcr condition. at the end i selected the magnesium ion density as 1. 25 mm. the production of rt - pcr was inserted directionally into pgem ? z ( ampr ). the pgem ? z ( ampr ) was used to transform e coli jm109. i got a positive clone through culling and identificatin. the dna sequence inserted into pgem ? z ( ampr ) was sequenced and blasted with the cdna sequence of the # - mannanase mature peptide that got from genbank

    分取誘導培養液中的菌體，用異硫氰酸胍法提取總rna ，總rna再經無rna酶的dna酶處理後用于rt ? pcr 。在pcr擴增目的基因時，通過優選擴增體系，使鎂離子濃度為1 . 25mm時rt ? pcr可順利地獲得目的基因，並能定向克隆到載體pgem ? 3z （ amp ~ r ）中。用克隆載體轉化宿主大腸桿菌jm109 ，通過篩選獲取陽性克隆子，對陽性克隆子進行酶切與pcr鑒定，並對載體中插入的目的基因進行測序。
  4. First, the purified pezzis and pcr product of angiostatin are digested by ecor. i and xba i. after purifying the digested products respectively, we ligate these two kinds of dna by t4 dna ligase and construct the recombinant plasmid pezz18 - as. then transform it to the competent e. coli dh5a

    用限制性內切酶ecori與xbai對目的基因as 、表達載體pezz18行雙酶切，酶切產物純化后利用大腸桿菌t _ 4dna連接酶連接構成重組子pezz18 - as ，並轉化e . colidh5 ，經氨芐青霉素lb平板初篩后，以菌液pcr和重組子的單、雙酶切行進一步鑒定。
  5. Water quality - detection and enumeration of intestinal enterococci in surface and waste water - miniaturized method most probable number for surface and waste water

    水質.表面和廢水中大腸桿菌計數和檢測.液體介質中培養的小型化方法
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