液體培養法 的英文怎麼說
中文拼音 [yètǐpéiyǎngfǎ]
液體培養法
英文
liquid culture method- 液 : 名詞(液體) liquid; fluid; juice
- 體 : 體構詞成分。
- 培 : 動詞1. (在根基部分堆上土) bank up with earth; earth up 2. (有目的地使成長、壯大) cultivate; foster; train
- 養 : Ⅰ動詞1 (供養) support; provide for 2 (飼養; 培植) raise; keep; grow 3 (生育) give birth to ...
- 法 : Ⅰ名詞1 (由國家制定或認可的行為規則的總稱) law 2 (方法; 方式) way; method; mode; means 3 (標...
- 液體 : liquid; liquor; fluid
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Materials and methods the mouse, golden hamster and human sperm were incubated with endotoxin in different concentration for different time to get capacitation, respectively, and ar was induced by progesterone after capacitation, then the rates of capacitation and ar were detected by chlortetracycline ( ctc ) and hoechst 33258 fluorescent staining method. the medium was with endotoxin in different concentration in sperm - oocyte fusion step during ivf, then the fertilization rate was observed. the 1 - cell, 2 - cell and zona - free 2 - cell mouse embryos were incubated in the medium with endotoxin, then the rate of blastocysts was recorded
方法取小鼠精子10份、金黃地鼠精子6份、人新鮮精液標本10份及人冷凍精液標本9份,分別與不同濃度內毒素共孵育進行體外獲能和孕酮誘導的頂體反應,應用金黴素和dna結合的熒光染料hoechest33258雙重熒光染色法檢測精子的獲能率和頂體反應率;小鼠體外受精實驗的精卵結合環節培養液中加入不同濃度的內毒素,觀察受精情況並記錄受精率;取小鼠1 -細胞胚胎、 2 -細胞胚胎和去卵透明帶2 -細胞胚胎,與不同濃度內毒素共孵育進行體外培養,觀察體外發育情況並記錄囊胚率。Effects of removal of the shoot apex and leaf wounding on nitrogen uptake and nicotine production were studied with tobacco plants grown in sand or hydroponic medium under the controlled conditions
摘要採用砂培和營養液培養方法,研究了在營養生長階段切除頂芽和葉片損傷對煙株生長、體內煙堿濃度、氮濃度及吸氮量的影響。This experiment passing to grope for the carbon source constitutes of the culture medium and using t. reesei rut c - 30 induced the expression of # - mannanase ( # - 1, 4 - mannan mannohydrolase ec 3. 2. 1. 78 ). in this experiment i put the constant carbon source ( lactose and locust bean gum ) in the foundation culture medium ( mandels nourishment liquid ) of t. reesei rut c - 30, then proceeded the variable carbon source ( dragon spruce fiber, com rush pith fiber, wheat straw fiber, wheat straw xylan, corn rush pith xylan, dragon spruce mannan ) to single factor, double factor, three factor, four factor and five factor orthogonal experiment. 1 determined the activity of p - mannanase using locost bean gum as substract by the 3, 5 - dinitosalicylic acid method, and observed the growing situation of the gernic at the end i selected the directions for the inducement expression of the # ? mannanase from trichoderma reesei rut - c30 that contained the dragon spruce fiber, wheat straw xylan, dragon spruce mannan
在里氏木霉rutc - 30的基礎培養基( mandels營養液)中加入固定碳源乳糖和槐豆膠,然後將可變碳源(雲杉纖維、玉米芯纖維、麥桿纖維、麥桿木聚糖、玉米芯木聚糖、雲杉甘露聚糖)進行單因子、雙因子、三因子、四因子、五因子的里氏木霉rutc - 30正交培養實驗,並以槐豆膠為底物用3 , 5二硝基水楊酸法測定培養液中?甘露聚糖酶的活力。從而確定了酶活最高且菌體生長良好的含雲杉纖維、麥桿木聚糖和雲杉甘露聚糖的誘導培養基為最佳培養基,用該培養基培養的里氏木霉( t . reesei ) rutc - 30使其轉錄的-甘露聚糖酶( - 1 , 4 - mannanmannohydrolaseec3 . 2 . 1 . 78 ) mrna量能夠滿足rt - pcr的要求。The deleted mutant pap gene was also cloned into yeast secreted expression ppic9k vector to form ppic9k ~ 3, then the vector was transferred into pachia pastoris gs115 strain. the specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50 - 60 u g per millilitre measured by uv - absorbed methods in the supernatant of the medium via high density fermentation. sds - page results showed that there was one protein band in the gel which molecular weight was about 34ku
將缺失型pap基因克隆于酵母分泌型表達載體ppicgk構成重組載體,然後導入畢赤酵母( p8chianastoris )菌株gslls細胞中,在甲醇的誘導下,經過酵母高密度發酵進行pap的表達,經sds page分析,結果表明,在培養基上清液中含有一明顯的特異性蛋臼條帶,大小為34ku ,經western blotting分析,該蛋白與法國pap抗血清有特異性反應,體外活性檢測表明該蛋白對tmv的侵染性具有高度的抑制性,說明該pap基因在畢赤酵母gs中也得到了正確表達。It was found that b - 6 isolated by the method of distinct zone of clearing in cellulose - congo red agar medium combined with measuring the enzyme activity of liquid culture filtrates had comparatively higher cellulase activity. by measuring activity of cellulase of strains growing in medium with different carbon sources and of washed mycelium induced by different carbon sources in certain time, it found that the formation of cellulase was regulated by the nature of the carbon source used for b - 6 and as3. 3711
真菌纖維素酶是一種誘導酶,碳源同時也是主要的誘導物來源,為了研究碳源對真菌纖維素酶合成的誘導機理,本文利用液體生長培養和洗滌菌絲誘導培養法研究了不同碳源對兩菌株的誘導特性,並用電泳分析法研究了不同碳源的誘導酶譜。Edible gelatine. detection of coliforms. 30 degrees c culture method on liquid selective medium
食用明膠.大腸桿菌的檢測.在30時液體選擇培養基的培養法Edible gelatine. determination of fecal coliforms. 44, 5 degrees c culture method on liquid selective medium
食用明膠.糞便中大腸桿菌的檢測.在44 . 5時液體選擇培養基的培養法Methods : in the dopa liquid medium, c. neoformans of all serotype strains including capsule - deficient strain were incubated and the d value were monitored under various conditions with a bausch and lomb spectronic 20
方法:在含左旋多巴的液體培養液中孵育各種血清型新生隱球菌,分光光度計測定不同條件下液體的d值。The information generated in current study suggests that the developing influenza ecosystem in southern china region may play an important role in the process of emerging novel influenza viruses, even directly impact the genesis of pandemic influenza strains. materials and methods : fecal, cloacal and tracheal swabs from different types of poultry were collected in live - bird retail markets once a week. they were inoculated into 9 - 11 days embryonated chicken eggs and incubated in 35 for 72 hours
本課題意在: 1 、以pa和cu為代表,探討hgnz亞型流感病毒在這些新形成的小種群中的流行情況; 2 、探討這些小種群中流感病毒的來源:是從其它動物跨種屬傳遞而來,還是本身為流感病毒的天然宿主: 3 、探討這些新形成種群中hgnz亞型流感病毒的進化情況,以及其在整個流感病毒生態體系中的作用;材料和方法:每周採集標本一次,常規處理后,接種於9一n日齡雞胚尿囊腔, 35恆溫培養72小時,收取雞胚尿囊液。In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company
實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 ( 1 : 1 )混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑( upofectalnineplusreageni )和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 ( do一7 )蛋白(即用型) ;鼠單克隆抗體p21waf , (即用型) ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰(濃縮型) ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。No production by mouse embryos embryos were cultured in hanks balanced salt solution for 4 hours. then the culture medium was collected, and equal amount of griess reagent was added into it. no concentrations were determined indirectly by spectrophotometry
胚胎發育過程中no的生成胚胎在hanks液中培養4小時后,取培養液加入等體積的griess試劑,用分光光度法檢測培養液中亞硝酸鹽的濃度,依此得知no的生成。The genotype is a main factor in the genetic transformation via agrobacteriwn - mediated. the results of orthogonal experiments of the factors which affect mainly the transformation illuminates that the erniuxin genotype + bacterium 15 x + explant + dip - dye is the best one. the cocultivation time is 28 days. the experiment shows that : the erniuxin cotyledon regeneration frequency is very high, and that the dongnong901 hypocotyl regeneration frequency is very high
確立了農桿菌介導轉化過程中基因型是影響轉化率的主要因素。通過極差分析,基因型為「二牛心」 ,菌液濃度15倍的,外植體為子葉,感染方法為浸泡法是最佳的組合。農桿菌與外植體共培養的時間為28h 。Used 15 nitrogen sources to select the fittest nitrogen source by solid culturate and liquid culturate
摘要選用15種常見氮源,應用固體和液體培養方法,篩選適宜番茄灰霉病菌生長的氮源營養物質。This paper studied the nutrient physiological characteristics and the liquid culture condition of cg rather completely
本論文採用各種試驗方法對土生空團菌的營養生理特性和液體培養條件進行了比較全面的研究。High performance ion exchange chromatography was applied in studying qualitatively and quantitatively of bacteria, which was shown as follows : firstly, physio - biochemical characteristics of bacteria was investigated by ion exchange chromatography. for the first time spores and nutrient of bacillus pumilus had been separated successfully by chromatography. chromatographial behaviors of bacteria at different cultivating environment and different growth phase were also studied
本文利用高效液相離子交換色譜系統研究細菌學,探討了該方法在細菌定性、定量方面的應用,主要包括三個方面:首先,利用離子交換色譜系統表徵細菌生理、生態方面的變化,首次成功地在色譜上區分了短小芽孢桿菌的芽孢及營養體;考察了不同的培養環境對細菌色譜行為的影響及不同生長階段的細菌的色譜行為。In order to investigate the tolerance of ectomycorrhizal fungi to heavy metals in vitro, three culture methods, namely liquid culture without agitation, liquid culture with agitation and solid agar culture, were investigated to determine which method would give the best combination of fungal biomass and ec50. the results indicated that liquid medium without agitation was the best culture method
為研究外生菌根真菌本身對重金屬污染的耐性,比較了液體靜置、液體搖床和瓊脂固體培養這三種常用的菌絲體的純培養方法,以真菌生物量大小和分離難易程度為主要指標,篩選出液體靜置方法為最優方法。The total rna was purified from the germ in the liquid by the guanidine isothiocyantehod method, then the total rna digested by dnase that had not rnase was used for rt - pcr. i change the magnesium ion dencity in the pcr system in order to optimize the pcr condition. at the end i selected the magnesium ion density as 1. 25 mm. the production of rt - pcr was inserted directionally into pgem ? z ( ampr ). the pgem ? z ( ampr ) was used to transform e coli jm109. i got a positive clone through culling and identificatin. the dna sequence inserted into pgem ? z ( ampr ) was sequenced and blasted with the cdna sequence of the # - mannanase mature peptide that got from genbank
分取誘導培養液中的菌體,用異硫氰酸胍法提取總rna ,總rna再經無rna酶的dna酶處理後用于rt ? pcr 。在pcr擴增目的基因時,通過優選擴增體系,使鎂離子濃度為1 . 25mm時rt ? pcr可順利地獲得目的基因,並能定向克隆到載體pgem ? 3z ( amp ~ r )中。用克隆載體轉化宿主大腸桿菌jm109 ,通過篩選獲取陽性克隆子,對陽性克隆子進行酶切與pcr鑒定,並對載體中插入的目的基因進行測序。Polysaccharide of mycelia from phellinus igniarius cultured by shaking were studied. lg9 ( 34 ) orthogonal experiment were carried out to find the optimum method of extracting polysaccharide from mycelia. the magnitude of range indicated that the factors which effected extracting ratio were times, time, temperature and proportion
本文對液體培養桑黃菌絲體的主要活性成分多糖進行了研究,首次利用lg9 ( 3 ~ 4 )正交試驗法對桑黃菌絲體多糖的提取工藝進行研究,從級差的大小可以看出影響桑黃菌絲體粗多糖提取率的因素主要是浸提次數,其次分別是浸提時間、吉林農業大學碩士學位論文桑黃主要生物學特性及多糖的研究浸提溫度、浸提比。Colchicine concentrations were four levels of 100, 500, 1000 and 2000 mg / l, and treatment periods were of 1, 2, 4 and 6 days for the former and 24, 48 and 72h for the latter. the results showed that both the two methods can induce polyploid cells, but the former was better than the latter in the induction rate and operating
經染色體數目鑒定表明,這兩種方法都能有效的誘導四倍體細胞( 2n = 4x = 48 )的產生,而二倍體染色體數為2n = 2x = 24 ,但是加入培養基法無論在誘導率上還是在操作上都優于液體浸泡法。All study aim is to lay a foundation for clinic appliance. methods : 1 ) hpfl was isolated by enzymatic digestion derived from rat fetal liver on ed13. 5d. furthermore, erythrocyte and other cell were removed from hpfl by erythrocyte - cracking solution and different attachment method
研究方法: 1 )取ed13 . 5d的大鼠胎肝,酶消化法離散細胞,用紅細胞裂解液去除紅細胞,差速貼壁法去除其它細胞,接種于不同的基質和培養液中, mtt法比較不同培養液和培養基質對大鼠hpfl的體外生長影響。分享友人