液體氰化 的英文怎麼說
中文拼音 [yètǐhuà]
液體氰化
英文
liquid cyaniding-
Photography chemicals - sodium thiocyanate crystals and sodium thiocyanate solution
攝影用化學藥品.硫氰酸鈉晶體和硫氰酸鈉溶液In the experiment, the first instar housefly larvae " metallothionein mrna expressing reach maximur. after induced by cd ( 2mm ) for 48 hours, then total rna was extracted with guanidinium isothiocyanate, and mrna was isolated by the polyattract mrna isolation systems surplied by promega company
試驗中採用2mmcd ~ 2對家蠅初孵幼蟲誘導48小時，使其體內金屬硫蛋白mrna表達達到高峰，在液氮中將其研磨成粉末后採用異硫氰酸胍法提取總rna ，再用promega公司提供的小量分離mrna試劑盒純化mrna 。The total rna was purified from the germ in the liquid by the guanidine isothiocyantehod method, then the total rna digested by dnase that had not rnase was used for rt - pcr. i change the magnesium ion dencity in the pcr system in order to optimize the pcr condition. at the end i selected the magnesium ion density as 1. 25 mm. the production of rt - pcr was inserted directionally into pgem ? z ( ampr ). the pgem ? z ( ampr ) was used to transform e coli jm109. i got a positive clone through culling and identificatin. the dna sequence inserted into pgem ? z ( ampr ) was sequenced and blasted with the cdna sequence of the # - mannanase mature peptide that got from genbank
分取誘導培養液中的菌體，用異硫氰酸胍法提取總rna ，總rna再經無rna酶的dna酶處理後用于rt ? pcr 。在pcr擴增目的基因時，通過優選擴增體系，使鎂離子濃度為1 . 25mm時rt ? pcr可順利地獲得目的基因，並能定向克隆到載體pgem ? 3z （ amp ~ r ）中。用克隆載體轉化宿主大腸桿菌jm109 ，通過篩選獲取陽性克隆子，對陽性克隆子進行酶切與pcr鑒定，並對載體中插入的目的基因進行測序。Cyanation of aryl halides is widely used in the synthesis of liquid crystals, but its traditional heating reactions require long time and high temperature
摘要鹵代芳烴的氰化反應在液晶單體合成中廣泛應用，但傳統的加熱方法反應時間長、溫度高。分享友人