淋菌素 的英文怎麼說
中文拼音 [lìnjūnsù]
淋菌素
英文
gonococcin-
Clinical comparative study of injectable fleroxacin and azithromycin in the treatment of non - gonococcal urethritis and cervicitis
鹽酸多西環素控釋微丸治療非淋菌性尿道炎和宮頸炎的療效觀察Results of all these 4 macrolides, the higher susceptibilities to gonococci were azithromycin and roxithromycin, in turn, the percentages of susceptibility were 100 %, 96. 04 %, respectively
結果在測定的4種大環內酯類藥物中，淋球菌對阿奇黴素敏感性最高，達100 % ；其次為羅紅黴素，為96 . 04 % 。The assay system of the biological activity of lymphotoxin was established using l929 cell as the sensitive target, lt international standard as the positive control and crystal violet staining method to detect viable cell after treated with lt. the best relationship between dosage and effect could be got if the cell seeding density in cell plate was 1. 6 0. 1 104 the dosage of amd was lug / ml, and the starting concentration of dilution in the plate of lt standard was 10 iu / ml with two fold dilution. the credibility of the established system was detected with rhtnfp developed by r & d
為確定經上述步驟純化后得到的目的蛋自lt 27的生物活性，本研究以l929細胞為靶細胞、淋巴毒素國際標準品為參照，採用結晶紫染色法檢測經淋巴毒素處理后存活的細胞，對淋巴毒素生物活性測定的細胞接種濃度、淋巴毒素標準品板上稀釋的起始濃度和梯度稀釋的倍數、放線菌素d的使用劑量等進行條件實驗后，建立了人淋巴毒素生物活性測定方法。Lymphotoxin ( lt ) is a kind of pleiotropic lymphocyte - secreted cytokine which mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. in order to increase the antitumor activity of lymphotoxin and reduce its side effects, the recombinant plasmid pet36b - lt 27 was constructed to express soluble fusion protein cbd - lt 27. the active form of lt 27 could be collected directly with several simple steps by three kind of components on the expressed fusion protein
本研究通過構建表達n端缺失27個氨基酸的淋巴毒素融合蛋白的重組質粒，在大腸桿菌中實現融合蛋白的可溶及分泌表達，同時利用表達載體上的幾種特殊序列經簡單的分離純化步驟直接獲得大量的有生物活性的淋巴毒素缺失體lt 27 ，為尋找一種高抗腫瘤活性、低臨床毒副作用的生物抗癌藥物進行了有效的探索。In order to further investigate the role of axudl in human tumor carcinogenesis and the potential association between the axudl gene expression status and the stimulation of transforming growth factor beta in human cancers, the present study was performed in three aspects as follows : ( 1 ) cloning full length enconding region cdna of axudl and construction of eukaryotic vector that expression the fusion protein of axud1 and influenza virus hemagglutin ha epitope tag ; ( 2 ) exploring the time and dose effects of tgf - 1 on the expression - of axudl gene in hepg2 hepatoma cells and spc - a1 lung carcinomas cells, and studying the effects of overexpression of axud1 on the expression of cell cycle and apoptosis related protein in hepg2 hepatoma cells ; ( 3 ) construction and expression of human axudl in e. coli m15. the following main results and conclusions can be obtained from the present study : 1. the full length ecnoding region of human axudl cdna from human peripheral blood lymphocytes was successfully cloned using one step rt - pcr method, and constructed into a eukaryotic expression vector which can be expressed a ha - axud1 fusion protein with axud1 and influenza virus hemagglutin ha epitope tag. the recombinant plasmid was identified by polymerase chain reaction, restriction endonuclease maping and sequencing, this expression vector might be instrumental to further study the function of axud1 protein in tumor cells
為了進一步研究axud1在人類腫瘤發生中的作用及axud1基因的表達狀況與tgf -介導的信號通路的關系，本實驗研究分為三個部分： （ 1 ） axud1基因cdna全長編碼區的克隆和ha表位標記的axud1基因表達載體的構建； （ 2 ）探討肝癌細胞hepg2和肺腺癌spc - a1細胞中tgf - 1誘導的axud1基因表達的時間、劑量效應以及誘導表達的可能機理，並研究axud1的過表達對細胞周期和細胞凋亡相關蛋白表達的影響； （ 3 ） axud1原核表達載體的構建及其在大腸桿菌中的表達。本實驗的主要結果和結論如下： 1利用一步法rt - pcr成功地從人類外周血淋巴細胞中克隆出axud1基因編碼區cdna ，並將其構建入真核表達載體中，編碼的ha - axud1融合蛋白帶有流感病毒凝血素ha的表位標記肽段。Umbilical cord blood samples were allowed to drain from the end of the cord into glass bottles with 20u / ml preservative - free heparin. the mononuclear cells were fractionated on a density gradient ( ficoll - hypaque, 1. 077g / ml )
無菌條件下採集肝素抗凝的正常人臍帶血，用相對密度1 . 077g ml的淋巴細胞分離液分離臍帶血中的單個核細胞，進而獲得msc 。This is not a common disease but when it happens, a group of people may be affected at the same time. sufferers will have red eyes, tearing and discharge, enlarged neck lymph nodes. as this is caused by a virus, there is no particular anti - microbial agent
發病的時侯，兩眼會突然發紅，常流眼淚，眼屎也多，耳朵周圍的淋巴結還會腫起來，由於這種眼病帶有病毒，所以沒有什麼特效藥，但為了防止細菌感染，有時可使用抗生素。In this paper, we mainly discuss the research progress of staphylococcal enterotoxin a in the aspects of the biological activities and its effect on promoting lymphocyte proliferation and inhibiting tumor cell growth
摘要該文就葡萄球菌腸毒素a的生物學活性、促淋巴細胞增殖和抑制腫瘤細胞生長作用的研究進展作一綜述。分享友人