溶細胞性抗體 的英文怎麼說
中文拼音 [róngxìbāoxìngkàngtǐ]
溶細胞性抗體
英文
cytolytic antibody- 溶 : 動詞(溶化; 溶解) dissolve
- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 胞 : Ⅰ名詞1 (胞衣) afterbirth2 (同一個國家或民族的人) fellow countryman; compatriot Ⅱ形容詞(同胞...
- 性 : Ⅰ名詞1 (性格) nature; character; disposition 2 (性能; 性質) property; quality 3 (性別) sex ...
- 抗 : Ⅰ動詞1 (抵抗; 抵擋) resist; combat; fight 2 (拒絕; 抗拒) refuse; defy 3 (對等) contend with...
- 體 : 體構詞成分。
- 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
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In contrast, n - acetyl - d - galactosamine, sucrose, d - glucose, glycogen, d - mannose, l - fucose and fetuin had no inhibitory effects on hemagglutination. so the lectin activity in the humoral fluids of amphioxus is sh group - dependent and is specifically inhibited by various d - galactosides. also, the humoral fluids obtained from e. coli - injected amphioxus showed increased agglutinating activity against human b and o, rabbit, grass carp and toad erythrocytes, but not against human a and chick erythrocytes, hinting that there might be two types of lectins in amphioxus humoral fluids
條紋斑竹鱉血清對懸浮在egta一mg一gvb緩沖液中的正常的兔血紅細胞、綿羊紅細胞、及部分哺乳動物、鳥類、兩棲類和魚類等代表動物的血紅細胞具有溶血活性;在gvbz十存在時對抗體包被的綿羊紅細胞具有溶血活性,而在edta一gvb緩沖液中不論是對正常的兔紅細胞或其他脊椎動物的紅細胞還是對抗體包被的敏感綿羊紅細胞都沒有溶血活性。The hemolytic activity was mg2 + - dependent and heat - sensitive, and was abrogated by treatment with rabbit anti - human c3 serum, zymosan, methylamine, hydrazine, and phenylmethylenesulfonyl fluoride ( pmsf )
文昌魚體液對兔血紅細胞的溶血活性在受到酵母聚糖、甲胺、肼、 pmsf 、 edta 、兔抗人補體3抗血清處理時,溶血活性消失。Activation of the complement may lead to killing of the microorganisms by direct lysis effect of complement system or the phagocytosis by the enhancement of the attachment of microbes to the phagocyte ( opsonization ). low serun concentrations of mbl in man is the basis for a common opsonic defect associated with recurrent infection
它可選擇性識別多種病原體表面的糖結構,以不依賴抗體和c1q的方式激活補體,發揮溶破和間接調理功能,還能與吞噬細胞膠凝素受體結合而起直接調理作用。Injected group, 0. 1 % saccharin ( 1. 5 ~ 2ml / rat, in 5min ) intraoral infused group and cta group. the expression of endogenous leucin - enkephalin ( lek ) in the rat brain was observed and 5 parts of the thalamus including laterodorsal thalamic nucleus ( ld ), lateral part of mediodorsal thalamic nucleus ( mdl ), ventroposterolateral thalamic nucleus ( vpl ), ventroposteromedial thalamic nucleus ( vpm ) and reticular thalamic nucleus ( rt ) were comparatively researched before and after the acquisition of cta applying lek - immunocytochemistry. in behavioral experiment, 18 adult male sd rats were divided into normal cta group ( control ) and 2 naloxone i. p
為探討cta形成過程中enk的作用,本實驗用成年雄性sd大鼠35隻,分為空白對照組、生理鹽水( 2體重)腹腔注射組、 0 . 15mlicl溶液( 2體重)腹腔注射組、 0 . 1糖精溶液口腔灌流組( 1 . 5 - 2ml只, 5min )和cta建立組,採用免疫細胞化學方法,觀察了亮腦啡肽( lek )陽性神經元在大鼠腦內的分佈情況,並比較了各組大鼠丘腦外側背核( ld ) 、丘腦內側背核外側部( mdl ) 、丘腦腹后外側核( vpl ) 、丘腦腹后內側核( vpm )以及丘腦網狀核( rt )等5個腦區內lek表達水平的差異;另外將成年雄性sd大鼠18隻,分為正常cta建立組以及在cta建立前或cta建立后阿片受體拮抗劑納洛酮( 2mg kg體重)腹腔注射組,對內源性阿片樣物質對于cta建立和保持的影響進行了行為學研究。After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s
通過細胞的免疫組化,細胞裂解物的sds - page電泳, westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示:重組質粒轉染的細胞質中有棕褐色顆粒,而空載體轉染細胞及正常細胞無此現象;細胞裂解物sds - page電泳結果顯示:只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶,這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致; western - blot分析結果顯示:約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清,成蟲蟲體可溶性抗原免疫兔血清, ts87基因原核表達蛋白免疫兔血清( ts87血清)以及一株具保護性的旋毛蟲單抗特異識別。The characteristics of this method are : a, directly counting cell number without the influence of the metabolic state of the cells ; b, discrimination of target cells from effector cells in cell - mediated cytotoxicity assay ; c, less treatment step, and free - radioactivity ; d, high sensitivity and reliability. 2, using the above assay, immunofluorescent labeled technique, and flow cytometry, the pbmc proliferation, apoptosis, necrosis, cell cycle, activation, cytokines and membrane marker were detected. the results showed that the number of pbmc reduced, but the activity of pbmc increased dose - dependently ; the reduction of cell number resulted from necrosis and apoptosis ; the supernatant of k562 cell lines were not able to block the cell cycle, but to promote it ; the ratio of t cell subset and the expression of thl and th2 cytokines increased
結合以上創建的方法和免疫熒光流式細胞術,用k562細胞株可溶性分泌物(上清)對外周血單個核細胞( pbmc )進行培養以模擬體內微環境,然後分別從細胞增殖、凋亡、壞死、細胞周期、活性、細胞因子和表面抗原表達等方面進行研究,結果發現用腫瘤上清培養的pbmc細胞數量下降明顯,但同時對其有激活作用,且呈劑量依賴性;細胞數的下降主要是由細胞壞死和凋亡引起的,腫瘤上清對細胞周期沒有阻斷作用,反而略有促進作用; t細胞亞群比例增加,並促進表達th1 、 th2細胞因子。Results showed that the muskrat musk increased the weight of unmatured mouse, enhanced weights of prostagland and sperm restore bladder, resisted hemolysis of red cell in higher or lower osmotic solutions, stabilized red cell membrane obviously, improved the activity of sod in the liver
本研究發現:麝鼠香具有促進未成齡小白鼠體重增長、增加小白鼠前列腺貯精囊的重量的作用;對抗小白鼠紅細胞在高滲和低滲液中溶血穩定紅細胞膜作用明顯,增強小白鼠肝臟中超氧化物歧化酶( sod )的活性。2 ) basis of upon studies, we have also designed and sythesized the mutation ii of the cmiv that been greatly changed in the 3 " of the gene comparing with nature cmiv : the ht gf3 ( the third loop region of htgf2 specifically binding to egfr receptor ) was fused to 3 " of gene of cmiv through a flexible linker. the gene of the mutation ii of cmiv was sequenced and clonged to the vector of ptxfus to fuse to the 3 " of gene of thioredoxin
二、在以上研究的基礎上,對cmiv抗菌肽的c端進行較大的改造,即將與腫瘤細胞過度表達的表皮生長因子受體( egfr )具有高親和性的因子多肽tgf _ 3通過疏水柔性接頭連接在抗菌肽cmiv的c端,設計完整的基因,並在大腸桿菌中利用ptxfus表達載體與硫氧還蛋白進行可溶性融合蛋白表達。The presence of complement - like activity in the humoral fluid of amphioxus was examined. the complement classical pathway ( cp ) activity is assayed by determining the amount of serum required to hemolyse a given number of sensitized sheep red blood cells ( srbc ), whereas the complement alternative pathway ( ap ) activity measured in the same manner, but by using rabbit red blood cells ( rarbc ) instead of sensitized srbc
文昌魚體液可溶解懸浮在10mmol legta - mg - gvb緩沖液中的正常的兔血紅細胞、綿羊紅細胞、及部分哺乳動物、鳥類、兩棲類和魚類的代表動物的血紅細胞;而在gvb ~ ( 2 + )緩沖液中時對抗體包被的綿羊紅細胞卻沒有溶血活性。The originalities of this paper are : 1, development of a a flow cytometry - based assay for quantitative analysis of cellular proliferation and cytotoxicity in vitro ; 2, the soluble secretion of k. 562 cell lines reduce the number of pbmc, but promote the activity of pbmc in dose - dependent manner ; 3, soluble secretion of k562 cell lines can induce the no production by pbmc, but no only plays a part of the role of soluble secretion of k562 cell lines ; 4, establishing a in vitro model and giving some parameters for sreening and appraising anti - tumor medicine
本研究的創新點在於: l 、建立了用流式細胞術定量測定細胞增殖和細胞毒性的直接檢測技術; 2發現k562可溶性分泌物劑量依賴性地使pbmc細胞數量減少但活性增加; 3 、 k562可溶性分泌物能誘導pbmc產生no ,但是no的作用並不等同於腫瘤上清的全部作用。 4 、為抗腫瘤藥物篩選提供了一個體外模型,並明確了一些篩選指標。Perioperative blood conservation interventions include use of antifibrinolytic drugs, selective use of off - pump coronary artery bypass graft surgery, routine use of a cell - saving device, and implementation of appropriate transfusion indications
圍術期血液保護干預措施包括應用抗纖溶藥,非體循環冠狀動脈搭橋手術的選擇性應用,應用常規的細胞挽救設備,並採用恰當的輸血指標。分享友人