烷化劑誘變 的英文怎麼說
中文拼音 [wánhuàjìyòubiàn]
烷化劑誘變
英文
alkylating agents mutagenesis-
Monofunctional alkylating agent n - methyl - n ' - nitro - n - nitrosoguanidine ( mnng ) is a widely spread environmental mutagen and carcinogen that targets dna and proteins to generate adducts. among the adducts, o6 - alkyl guanine is the predominant mutagenic lesion because of its mispairing properties, which can eventually lead to chromosomal aberrations, point mutations, and cell death. this lesion also appears to be involved in tumor initiation, particularly in gastric carcinogenesis
單功能烷化劑n -甲基- n -硝基- n -亞硝基胍（ mnng ）是一種在環境中廣泛存在的化學誘變劑和致癌劑，它能和dna及蛋白質等生物大分子形成加合物（ adduct ） ，其引起的與突變有關的主要dna損傷類型是o ~ 6 -甲基鳥嘌呤，這種損傷與腫瘤尤其是胃癌的發生密切相關。Therefore, it is important to study the profiles of gene expression, which will help understand the global cellular stress responses to chemical carcinogens, and further elucidate the mechanisms of nontargeted mutagenesis
所以，從整體上研究烷化劑作用后細胞基因表達譜改變，對于了解化學致癌物誘發的哺乳動物細胞應激反應的全貌和揭示非定標性突變的發生機制具有非常重要的意義。In our laboratory, a unique mutation detection system using a shuttle vector plasmid has been established to demonstrate that a low concentration of mnng ( 0. 2 m ) can induce nontargeted mutation in mammalian cells : the mammalian cells were exposed to 0. 2m mnng for 2. 5h, then a shuttle plasmid pz189 carrying supf trna gene was transfected into cells after 24h culture. we found a 5 - fold higher mutation frequency of the plasmid replicated in pretreated cells than the spontaneous mutation frequency of the plasmid replicated in control cells. this kind of mutation did not occur immediately after mnng exposure
我們實驗室曾用一特殊的突變檢測系統，直接證明dna損傷劑可在哺乳動物細胞誘發非定標性突變：首先用低濃度（ 0 . 2 m ）的短壽烷化劑mnng （半壽期為1 . 1hr ）處理細胞2 . 5h后，繼續培養24h ，將重組有用作突變檢測的靶基因supftrna基因的穿梭質粒pz189轉入細胞復制，發現在未受致癌物直接攻擊的穿梭質粒中有較自發突變率高5倍以上的靶基因突變。
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