無菌培養 的英文怎麼說

中文拼音 [wújūnpéiyǎng]
無菌培養 英文
aseptic culture
  • 無 : 無Ⅰ動詞（沒有） not have; there is not; be without Ⅱ名詞1 （沒有） nothing; nil 2 （姓氏） a surn...
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 培 : 動詞1. （在根基部分堆上土） bank up with earth; earth up 2. （有目的地使成長、壯大） cultivate; foster; train
  • 養 : Ⅰ動詞1 （供養） support; provide for 2 （飼養; 培植） raise; keep; grow 3 （生育） give birth to ...
  • 無菌 : [醫學] asepsis; sterility; germ free; sterile無菌操作法 aseptic manipulation; 無菌隔離室 germfree...
  1. Abstract : the total quantity of perylene quinonoids in hypocrella bambusae ( b. et br. ) sacc., shiraia bambusicola p. henn and culture substance of ascomycetes hypocreacae ( fr ) tul. sp. were determined with spectrophotometric methods. the relative extraction efficiency were studied when ethanol, acetone and chloroform were used as solvents. among them acetone was the best one

    文摘:用分光光度法測定了竹紅菌、竹黃和菌寄生菌培養物中?醌類化合物的總量；同時研究了以無水乙醇、丙酮和氯仿為溶劑提取?醌類化合物的相對效率，結果發現以丙酮為溶劑最合適。
  2. Obtaining transgenic male sterile tobacco in order to prove that hsp70 antisense cdna can lead to male sterility, with plasmid 3301 + 650, 3301 + 651 we transformed 207 aspetic tobacco leaves by genegun bombarding and agrobacterium mediation ( 109 by genegun bombarding, 98 by agrobacterium ). by cultivating them in blotting media containing basta 0. 4 mg / 1, we get 181 resistant leaves ( 98 by genegun bombarding, 88 by agrobacterium mediating )

    獲得轉基因雄性不育煙草為了證實hsp70反義cdna能創造雄性不育，我們將3301 + 650和3301 + 651質粒用基因槍和農桿菌介導法轉化煙草無菌發芽的葉片，共207片（基因槍109片，農桿菌98片） 。在含basta0 . 4mg l的篩選培養基上進行篩選，得到抗性葉片181片（基因槍93片，農桿菌88片） 。
  3. Isolate all grew well in the culture medium with initial ph 4 - 10, the optimal growth temperature range was from 28 to 30. 5 degree c. it grew well on the medium for fungi growth, such as pda medium and czpek medium etc, and also grew well on the cause ' s i medium and the non - nitrogen medium, but little growth on the luria bertani medium ( lb ) and beef extract peptone medium. it did not need special nutrition factors for growth, but source of the carbon was the key factor to growth, all of its nutrition needs were different from that of common bacteria

    該菌在初始ph4 - 10的培養基中都能夠生長，生長最適溫度范圍為28 - 30 . 5 ，在pda 、查氏等真菌培養基中生長旺盛，在高氏1號和無氮源培養基中同樣生長良好，而在lb與牛肉膏蛋白腖等細菌培養基中生長很差，碳源是其生長的關鍵因子，這有別於一般細菌的營養需求。
  4. Using the mouse fetal ovary serum - free culture model, fetal ovaries from 14 day post coitus ( 14 dpc ) mouse were cultured, and treated by ay9944 - a - 7, nystatin and rs - 21745. the results showed that 0. 025, 0. 0625 and 0. 125 um ay9944 - a - 7 or 25, 50 and 75 iu / ml nystatin increased the total number of follicles per ovary significantly ; however, ay9944 - a - 7 and nystatin at the same doses could n ' t cause the same effect on the number of growing follicles and the average diameter of five largest follicles per ovary. 50 u. m rs - 21745 decreased the total number of follicles, the number of growing follicles and diameter of follicles per ovary significantly after 48 h

    首先利用小鼠胚胎卵巢的體外無血清培養模型，培養妊娠14天（ 14daypost - coitus ， 14dpc ）小鼠胚胎卵巢，分別添加能促進mas積累的ay9944 ，制黴菌素，和能抑制mas產生的rs - 21745進行處理，結果表明： 0 . 025 、 0 . 0625利0 . 125 m的ay9944 - a - 7與25 、 50和75iu ml的制黴菌素能顯著提高卵巢中形成卵泡的總數量，但是對生長卵泡數和卵泡直徑的作用不同；而mas合成抑制劑rs - 21745能夠顯著降低形成卵泡的總數量。
  5. These two cases had cloudy peritoneal fluid with high eosinophilic count without abdominal pain, and negative of peritoneal fluid culture and gram stain

    這些病人臨床上的特徵有腹膜透析液混濁、嗜伊紅性白血球增高，但無腹痛且腹膜透析液的培養及革蘭氏細菌染色均正常。
  6. Here we studied the relationship of various factors and the quality of protoplasts. which maybe could be the basic of moss gene targeting. results showed : inoculated the spores onto diferrent kinds of media, such as ms, benecke and knop, we found that there was no difference when the spores germinated and differentiated into cauliform soon

    通過對立碗蘚的無菌培養和原生質體操作發現： （ 1 ）立碗蘚孢朔接種在無菌ms 、 benecke 、 knop培養基上，均可萌發產生原絲體，但不久便分化為莖葉體，很難長期保持其原絲體狀態，不同培養基條件下原絲體狀態有所不同。
  7. Carrot tissue culture and plant regeneration factors including explants, medium and culture condition are combined together to study the most efficient protocol of carrot tissue culture and plant regeneration thereof. the most suitable explant is fresh hypocotyls segment and precultured hypocotyls derived from 7 - 10 day old aseptic plantlets generating in dark or in dim light, the best recipe for cullus induction and subculture is b5c ( 85 with 0. 5mg / l 6ba and 0. 5mg / l 2, 4 - d ), the ideal recipe for plant regeneration is 65 or ms free of hormone. a phytotron with a 16 / 8 h day / night cycle, at 25 is feasible for plant regeneration, and occasional exposure to sun light dramatically stimulates plant growth

    建立了高效的胡蘿卜組織培養及再生體系以適于生產飲料的胡蘿卜「新黑田五寸人參」為材料，研究不同外植體、不同培養基，不同培養條件對胡蘿卜愈傷誘導及再生的影響，建立一套高效的胡蘿卜組織培養再生體系：最適于誘導愈傷的外植體是弱光或黑暗下發芽7 - 10d無菌苗下胚軸，最適合的愈傷誘導培養基和繼代培養是b _ 5c （ b _ 5 + 0 . 5mg l6ba + 0 . 5mg l2 ， 4 - d ） ，最適于植株再生的培養基為不添加任何激素的b _ 5或ms ，組織培養條件為25 、光照周期為16hr 8hr 。
  8. Spawn is necessary for both cultivation of edible fungi and tameness of wild mushroom. the reliable method of spawn identification is to produce carpophores by the culture, which is not only time - consuming for domestic fungi but also unuseful for untamed wild mushroom

    無論是食用菌的人工栽培，還是珍貴野生蘑菇的馴化研究，都需要制備菌種並對菌種的真偽進行鑒定，其中最可靠的鑒定方法是誘導純培養菌種產生賴以識別的子實體。
  9. The regeneration system of tobacco was established. the adventitious shoots were induced from leaf explants of tobacco based on ms basal medium supple - - mented with 2. 0mg / l 6 - ba and 0. 3mg / l naa. then the regenerated plants were rooted on ms medium containing 0. 3mg / l naa

    植株再生體系的建立採用無菌苗煙草葉片為外植體，以ms為基本培養基，篩選出ms + 6 - ba2 . 0mg l + naa0 . 3mg l以誘導不定芽的分化，並於ms + naa0 . 3mg l的pa碩士學位論文v沉了砂「工隊nk 」主根培養基上生根，獲得完整再生植株。
  10. Leaves of tobacco ( nicotiana tabacum ) were wounded, infected by agrobacterium tumefaciens strain lba4404 and gv3101 : pmp90 harboring expression vector pbgsb and pbglsb and co - cultured for 3 days in darkness on the culture medium ( ms + 0. 5mg / l 6 - ba + 0. 7 % agor ph 5. 7 )

    4以煙草（品種sr ）無菌苗葉片為外植體，採用農桿菌浸染的葉盤轉化法，用構建好的植物表達載體對煙草進行遺傳轉化。外植體置於無抗生素的誘芽培養基（ ms 0
  11. Results showed that in the water body of xizi lake, annual average of culturable planktonic ammonifiers and nitrogen fixers were 510 and 236 cfu / ml, respectively ; ammonia oxidizers, nitrite oxidizers, nitrate reducers and denitrifiers were 8. 5, 16, 587 and 16 mpn / ml, respectively ; inorganic phosphate solubilizing bacteria ( 1pb ) and organic phosphorus mineralizing bacteria ( opb ) were 89 cfu / ml and 37 mpn / ml, aerobic and anaerobic cellulose decomposers were 7 and 5 mpn / ml, respectively

    水體中可培養異養細菌（氨化細菌）和固氮菌的年平均值分別為510和236cfu ml ，氨氧化細菌、亞硝酸氧化細菌、硝酸鹽還原菌和脫氮菌的數量分別為8 . 5 、 16 、 587和16mpn ml ；無機磷和有機磷分解菌分別為89cfu ml和37mpn ml ；好氧性纖維素分解菌和厭氧性纖維素分解菌只有7和5mpn ml 。
  12. Pcr amplification using 2 degenerate primers for nitrogenase fe protein gene was performed with chromosomal dna isolated from the 29 isolates. the result suggested that a nifh amplicon of 323 nucleotides was detected in 7 isolates and the 7 isolates are c4 c5 g1 g2, w5 t1 and t7. these pcr amplified fragments were cloned, and sequenced

    首先利用芽孢桿菌中芽孢的抗熱性將土壤溶液在100沸水中煮10 - 15分鐘，然後用選擇性無氮培養基進行初篩得到29株菌落形態不同的菌株；接著用固氮酶結構基因nifh的特異性引物對這29株菌進行pcr擴增，結果表明其中7個菌株具有nifh基因，這7個菌株的編號依次為： c4 、 c5 、 g1 、 g2 、 w5 、 t1和t7 。
  13. Pepper ( capsicum annuum l. ) is one of the important vegetables and condiments. in order to laying down the foundation for the selecting and breeding of polyploid of pepper, a experiment of induction polyploids of pepper by colchicines treatment in clones of pepper was carried out

    本實驗用萌動的辣椒種子在ms培養基中培養獲得無菌苗，取下胚軸為外植體，在ms + 6 - ba2 . 0mg l + iaa1 . 0mg l培養基中誘導形成愈傷組織。
  14. Effects of diverse environmental factors on the growth rate ( od4oo ) and nitrogenase activity ( ara ) of the strain w12 hi nitrogen - free culture were investigated in our experiments. the results implied that the strain w12 could easily adapt to different cultural conditions : it could use various carbon sources ( especially glucose, sucrose, malic acid, mannitol ), propagate quickly and fix nitrogen at a temperature range of 15 ? to 40 ? and at 25 - 35 ? for optimum, at a ph range of 4 to 8. 5, at a saline concentration range of 0. 01 % to 1. 5 % ; low nlv " concentration had little effect on its nitrogenase activity. ara could also be detected when it grow in the culture media with 5mmol / l ntv "

    W12菌株對環境因子的適應性研究：無氮培養條件下，測定溫度、碳源、酸堿度、滲透壓對w12生長及固氮能力的影響，結果表明，在15 - 40下均能生長並表達固氮酶活性，其最適生長及固氮的溫度為25 - 35 ；能利用葡萄糖、蔗糖、蘋果酸、甘露醇等多種碳源生長並固氮，當培養基中同時存在蔗糖和蘋果酸時，細菌生長和固氮活性最強；在偏酸和偏堿的條件下（ ph4 . 5 - 8 . 5 ）均能保持較強的生長勢和較高的固氮酶活性，並能通過調節自身代謝平衡並適應環境的酸、堿性變化，使培養液趨于中性：能耐受較高的滲透壓，培養液中卜、 5 naci濃度對其生長和固氮酶活性影響不大，當naci濃度升至2時，菌株的生長勢及固氮酶活性才有所下降：低濃度的鉸對其固氮酶活性影響不大，在0
  15. In this thesis, physcomitrium sphaericum growing in the area of beijing, which belongs to the same section as physcomitrella patens, is applied as experiment material to explore the condition of the sterile culture

    本論文採用生長在北京地區的與小立碗蘚同科立碗蘚（ physcomitriumsphaericum ）為材料，探討其無菌培養的條件。
  16. After co - cultivation, all the explants were washed in liquid mso medium containing 500mg / l cef and 500mg / l carb for 2 hours. washed with sterile water for 5 times and patted dry on the filter paper again

    共培養后，以附加有500mg / l的cef和500mg / l的carb的ms0液體培養基震蕩脫菌培養2小時，無菌水沖洗5次，無菌濾紙吸干水分。
  17. Uniform design was employed to optimize the culture conditions and the component of culture medium including carbon source, nitrogen source, phosphor source, growth factors, inorganic salts and precusor as well

    利用均勻設計原理進行實驗設計，以優化培養基中碳源、氮源、磷源、生長因子、前體物及無機鹽成份的配方以及細菌培養條件。
  18. Results. the 120 specimens from 30 patients underwent bacterial culture growth : 116 were sterile, an 4 aerobic cultures ( 2 patients ) grew coagulase - negatie staphylococci, suggestie of contamination

    結果：這來自30個病人的120片碎片在經歷了細菌培養程序后發現： 116片是無菌的，有4片（來自2個病人）在有氧條件下培養除了凝固酶陰性的葡萄球菌，提示被污染了。
  19. The ammonia - oxidizers were increased from 2. 13 106 / g mlss to 6. 28 108 / g mlss, while the nitrite - oxidizers were increased from 2. 13 103 / g mlss to 6. 28 106 / g mlss. after a month, most of the heteotrophic bacteria were washed out from the system. they nitrifying bacteria were prevalent in the enrichment system and were around 99 % in total bacteria

    選取富含硝化細菌的活性污泥作為富集培養的對象，採用純無機培養基對硝化細菌進行定向富集培養，能在較短的時間內得到大量硝化細菌富集培養物，硝化細菌數約占總菌數的99以上。
  20. In nitrogen - free medium, the logarithmic phase of the strain w12 was about 13 - 48 h. in logarithmic period its doubling time was 2 - 4 h in nitrogen - free medium, 0. 9 - lh when enough nk

    在無氮培養基上該菌在13h后進入對數生長期，並持續至48 52h ，這期間其生長代時為2 - 4h ，有氮培養基上為0 . 9 - 1h 。
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