無菌條件 的英文怎麼說

中文拼音 [wújūntiáojiàn]
無菌條件 英文
aseptic condition
  • 無 : 無Ⅰ動詞（沒有） not have; there is not; be without Ⅱ名詞1 （沒有） nothing; nil 2 （姓氏） a surn...
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 條 : Ⅰ名詞1 （細長的樹枝） twig 2 （條子） slip; strip 3 （分項目的） item; article 4 （層次; 秩序; 條...
  • 件 : Ⅰ量詞（用於個體事物） piece; article; item Ⅱ名詞1. （指可以一一計算的事物） 2. （文件） letter; correspondence; paper; document
  • 無菌 : [醫學] asepsis; sterility; germ free; sterile無菌操作法 aseptic manipulation; 無菌隔離室 germfree...
  • 條件 : 1. （客觀的因素） condition; term; factor 2. （提出的要求） requirement; prerequisite; qualification
  1. After analyzing purple soil sampled from neijiang, leshan ofsichuan provinec and yuanmou of yunnan province, the contrast results of microbe quantity feature between surface and subsurface purple soil were obtained as follows. the content of soil organic matter, total and available nitrogen, phosphorous and potassium of surface was higher than subsurface, not relating to the type of purple soil and soil utilization way. the quantity of microbe _ bacteria, actinomyces and mould in surface purple soil was higher than subsurface, which indicated that the organic matter and airy condition in surface soil was more suitable for microbes growing. there was the same tendency in profile change of microbe quantity in purple soil located in temperate _ humid climate of sichuan basin in contrast with dry _ hot climate of yuanmou, yunnan. the nutrient situation of purple soil in sichuan basin shown that state of surface was better than subsurface, while in yuanmou of yunnan the state was on the contrary due to the degradation of surface soil

    實驗室對四川內江、樂山和雲南元謀不同類型紫色土表層和亞表層微生物數量特性的比較分析表明：土壤有機質、氮磷鉀全量及其速效量均表現為表層高於亞表層，與紫色土類型和利用方式無關；三大類土壤微生物細菌、放線菌和黴菌數量均表現出表層高於亞表層，表明紫色土表層的有機質和通氣性優于亞表層，適宜於這三大類微生物生長；溫濕氣候條件下的四川盆地和乾熱氣候條件下的雲南元謀其紫色土微生物數量的剖面變化具有相同的趨勢，唯土壤營養狀況在四川盆地紫色土中表現為表層優于亞表層，而在雲南元謀紫色土中由於表層土壤的退化作用表現為亞表層優于表層的相反情況。
  2. Ammonifiers in suzhou creek can not use inorganic nitrogen and carbamide as nitrogen source ; additional carbon source and garbage lixivium have little influence on ammonifiers growth ; high content of salt and low temperature restrain ammonifiers growth ; alkalescent condition has little influence on ammonifiers, but acidic condition restrain ammonifiers growth ; the biomass of ammonifiers are not necessary correlated with the function of ammonifiers, adding glucose with 1g / l content into the water sample obviously promote the growth and function of ammonifiers. physiological groups of bacteria play significant role in the translation and

    蘇州河的氨化菌無法利用無機氮和尿素作為氮源；在營養條件充足時添加額外c源和富含有機物的垃圾浸出液對氨化菌的生長基本無影響；高鹽度和低溫抑制氨化菌生長；堿性條件對氨化菌的生長影響不大，酸性條件對氨化菌生長具有抑制作用；氨化菌生物量的消長與轉氨活性之間不存在必然聯系， 1g / l的葡萄糖對蘇州河水樣中氨化菌的數量和轉氨功能具有明顯的促進作用。
  3. Here we studied the relationship of various factors and the quality of protoplasts. which maybe could be the basic of moss gene targeting. results showed : inoculated the spores onto diferrent kinds of media, such as ms, benecke and knop, we found that there was no difference when the spores germinated and differentiated into cauliform soon

    通過對立碗蘚的無菌培養和原生質體操作發現： （ 1 ）立碗蘚孢朔接種在無菌ms 、 benecke 、 knop培養基上，均可萌發產生原絲體，但不久便分化為莖葉體，很難長期保持其原絲體狀態，不同培養基條件下原絲體狀態有所不同。
  4. Carrot tissue culture and plant regeneration factors including explants, medium and culture condition are combined together to study the most efficient protocol of carrot tissue culture and plant regeneration thereof. the most suitable explant is fresh hypocotyls segment and precultured hypocotyls derived from 7 - 10 day old aseptic plantlets generating in dark or in dim light, the best recipe for cullus induction and subculture is b5c ( 85 with 0. 5mg / l 6ba and 0. 5mg / l 2, 4 - d ), the ideal recipe for plant regeneration is 65 or ms free of hormone. a phytotron with a 16 / 8 h day / night cycle, at 25 is feasible for plant regeneration, and occasional exposure to sun light dramatically stimulates plant growth

    建立了高效的胡蘿卜組織培養及再生體系以適于生產飲料的胡蘿卜「新黑田五寸人參」為材料，研究不同外植體、不同培養基，不同培養條件對胡蘿卜愈傷誘導及再生的影響，建立一套高效的胡蘿卜組織培養再生體系：最適于誘導愈傷的外植體是弱光或黑暗下發芽7 - 10d無菌苗下胚軸，最適合的愈傷誘導培養基和繼代培養是b _ 5c （ b _ 5 + 0 . 5mg l6ba + 0 . 5mg l2 ， 4 - d ） ，最適于植株再生的培養基為不添加任何激素的b _ 5或ms ，組織培養條件為25 、光照周期為16hr 8hr 。
  5. The carbofuran - degrading experiment of cds - 1 was carried out in lab scale, the results showed that the highest degrading efficiency was obtained with ph, temperature being 7. 0, 30c respectively ; the change of aeration had no influece on degrading rate ; the increasing inocula could accelerate carbofuran degrading progress ; the degrading capability of cds - 1 was n ' t inhibited by high carbofuran concentration ; the addition of low concentration of nutrients had no distinct effect on the degrading rate while high concentration had inhibiting effect the distribution of degrading enzyme was also primarily studied, the results showed that degrading - related enzyme was endocellular and degrading progress was not cometabolism

    Cds - 1的降解酶（系）是誘導酶（系） ，存在明顯的誘導期；胞內、胞外酶實驗表明呋喃丹降解酶（系）存在於細胞內。添加低濃度外源營養物質對cds - 1的降解性能無明顯影響，說明cds - 1降解呋喃丹的過程不屬于共代謝過程，可以在無外源營養物質存在的條件下降解呋喃丹。添加高濃度外源營養物質會對該菌降解性能產生抑制。
  6. Effects of diverse environmental factors on the growth rate ( od4oo ) and nitrogenase activity ( ara ) of the strain w12 hi nitrogen - free culture were investigated in our experiments. the results implied that the strain w12 could easily adapt to different cultural conditions : it could use various carbon sources ( especially glucose, sucrose, malic acid, mannitol ), propagate quickly and fix nitrogen at a temperature range of 15 ? to 40 ? and at 25 - 35 ? for optimum, at a ph range of 4 to 8. 5, at a saline concentration range of 0. 01 % to 1. 5 % ; low nlv " concentration had little effect on its nitrogenase activity. ara could also be detected when it grow in the culture media with 5mmol / l ntv "

    W12菌株對環境因子的適應性研究：無氮培養條件下，測定溫度、碳源、酸堿度、滲透壓對w12生長及固氮能力的影響，結果表明，在15 - 40下均能生長並表達固氮酶活性，其最適生長及固氮的溫度為25 - 35 ；能利用葡萄糖、蔗糖、蘋果酸、甘露醇等多種碳源生長並固氮，當培養基中同時存在蔗糖和蘋果酸時，細菌生長和固氮活性最強；在偏酸和偏堿的條件下（ ph4 . 5 - 8 . 5 ）均能保持較強的生長勢和較高的固氮酶活性，並能通過調節自身代謝平衡並適應環境的酸、堿性變化，使培養液趨于中性：能耐受較高的滲透壓，培養液中卜、 5 naci濃度對其生長和固氮酶活性影響不大，當naci濃度升至2時，菌株的生長勢及固氮酶活性才有所下降：低濃度的鉸對其固氮酶活性影響不大，在0
  7. The viable cells after counting with trypan blue dye exclusion were then transferred to culture flask containing dmem medium in a density of 1 10 ^ 6

    方法無菌條件下，從6月齡紐西蘭白兔的膝關節囊內剪取滑膜組織，採用組織塊培養法和酶消化法分離滑膜細胞。
  8. In this experiment, radio - immunoassay and hybridization in situ were applied to observe the insulinotropic activities of glp - 1 ( 7 - 36 ) nh2 and reveal the mechanisms underlying this process. methods : rat pancreases were removed from 3 - 5 day - old sprague - dawley rats and dissected into 0. 5mm3 segments and islets were isolated by the collagenase digestion method of wangling et al. thoroughly washed islets and suspended in modified rpmi - 1640 medium supplemented with 10 % fetal bovine serum, and added to 50ml cell culture flasks

    方法：胰島的分離參照王玲等的方法，每次實驗取新生3 - 5天sd大鼠，無菌條件下剖腹取出胰腺，剪切為0 . 5mm ~ 3的組織塊， v型膠原酶消化30min后，離心洗滌，懸浮於完全培養基，接種入50ml培養瓶，於5 co _ 2 、 95空氣條件下培養20h ，轉板純化，接種於96孔培養板培養24h ，按實驗要求進行實驗。
  9. This article introduces the production of edible fungi secondary spawn and culture spawn from preparation of substrate, medium sterilization, sterile working technique, cultural condition, cultural inspection and storage condition in detail

    摘要從配料、滅菌、無菌操作技術、培養條件、培養檢查、貯藏條件幾個方面對食用菌原種和栽培種生產工藝技術要求進行了詳細陳述。
  10. In this thesis, physcomitrium sphaericum growing in the area of beijing, which belongs to the same section as physcomitrella patens, is applied as experiment material to explore the condition of the sterile culture

    本論文採用生長在北京地區的與小立碗蘚同科立碗蘚（ physcomitriumsphaericum ）為材料，探討其無菌培養的條件。
  11. Sudies on the trannsmission of phytophthora root rot of soybean had been carriedout with the soil debris and the seeds which came from natural diseased fields as well as thesoil and seeds which inoculated with the pathogen

    對帶菌土壤、病殘體進行傳病試驗，研究了人工接種條件下種子帶菌條件及帶菌種子傳病情況，結果表明，帶菌土壤和病殘體無論是在當年還是越冬后都可有效地傳播大豆疫霉根腐病，是大豆疫霉根腐病的主要初侵染源和傳播途徑。
  12. We can see a lot of new pictures of induced neuron - like cells, include neuron - like cells and glial - like cells, with the apparent nerve cells. material and methods rat bone marrow cell were collected, after sacrifice of a 3 months old wistar rat, from femurs and tibias by flushing the shaft with culture media ( dmem - 10 % fetal bovine serum ) using a syringe of 5ml

    無菌條件下取股骨和胚骨的骨髓b人snd含10胎牛血清的dmem培養液1000rpm離心10分鐘，吹打b人兩個培養瓶，置人37 j coh飽和濕度條件下培養，隔日更換培養液，當細胞達到70融合時，傳代。
  13. Parametric release can be authorised if the data demonstrating correct processing of the batch provides sufficient assurance, on its own, that the process designed and validated to ensure the sterility of the product has been delivered

    達到以下條件后可以授權給參數放行：顯示正確處理這一批的數據能獨立提供足夠的保證，以上所述的處理是設計和驗證好的，以確保無菌產品已經送達
  14. The different organs of elaeagnus mollis diels were used in this experiment as the explants. after establishing the aseptic propagation system, the aseptic tube stems and leaves were used to study the effect of different exogenous hormones combination and different culture conditions on organgenesis. the optimum medium and culture condition was screened for fast propagation of elaeagnus mollis diels

    本實驗選材為胡頹子科胡頹子屬的翅果油樹，在建立起無菌繁殖系的基礎上，分別以試管無菌莖段和葉片為材料，研究了不同外源激素配比及不同培養條件對其器官分化的影響，從中篩選出適合翅果油樹快速繁殖的最適培養基和培養條件。
  15. Bone under sterile conditions can be deep frozen ( - 70 - 80 ) for storage

    骨質在無菌條件下可通過深凍（ - 70 - 80 ）保存。
  16. The feeding of glutamic acid, glutamine and a - keto - glutaric acid respectively showed that glutamic acid and glutamine had no obvious effects on cell growth, but stimulated apramycin production greatly. with the same concentration of amino acids supplemented, glutamine showed a stronger stimulation effect than glutamic acid, while a - keto - glutaric acid showed a repression effect on apramycin production. it could be deduced from above results that glutamine possibly is the donor of nitrogen element for the biosynthesis of apramycin

    Glu 、 gln及?酮戊二酸添加實驗結果表明glu 、 gln對菌體的生長無明顯的影響，但能強烈促進安普黴素的生物合成，且相同濃度條件下gln的促進作用明顯高於glu ，而?酮戊二酸則對安普黴素的生物合成有抑制作用。
  17. Human bone marrow samples were obtained from normal person. bone marrow mononuclear cells were isolated with 1. 073g / ml percoll. mscs were obtained by removing the non - adherent cells

    無菌條件下採集正常人的骨髓，用相對密度1 . 073g ml的percoll分離液分離骨髓中的單個核細胞，進而獲得msc 。
  18. Umbilical cord blood samples were allowed to drain from the end of the cord into glass bottles with 20u / ml preservative - free heparin. the mononuclear cells were fractionated on a density gradient ( ficoll - hypaque, 1. 077g / ml )

    無菌條件下採集肝素抗凝的正常人臍帶血，用相對密度1 . 077g ml的淋巴細胞分離液分離臍帶血中的單個核細胞，進而獲得msc 。
  19. 4. effect of resolution vector on the expression of pesticidal crystal protein genes this work successfully introduced pesticidal crystal protein genes into crystal negative strain bmb171 by using resolution shuttle vectors. after resolution, the expression of cry1ac and cry 1c genes have no obvious change, but the expression of cry3a genes has great increase in the same condition

    解離反應對殺蟲晶體蛋白基因表達的影響成功地利用解離載體將crylac10 ， crylc和cry3a基因導入蘇雲金芽胞桿菌無晶體突變株， crylac和crylc基因解離前後的表達量和殺蟲毒力未見明顯變化， cry3a基因在相同條件下則表達量有所提高，至於為何只對基因cry3a有作用尚不清楚，國內外也未見有人作相關報道。
  20. Capable of functioning under varying environmental conditions. used of certain organisms, such as bacteria that can live with or without oxygen

    兼性寄生的能在不同環境條件下活動的。用於某些生物，如細菌在有氧或無氧條件下都能生存
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