無蛋白免疫原 的英文怎麼說
中文拼音 [wúdànbáimiǎnyìyuán]
無蛋白免疫原
英文
isopatin- 無 : 無Ⅰ動詞（沒有） not have; there is not; be without Ⅱ名詞1 （沒有） nothing; nil 2 （姓氏） a surn...
- 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
- 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
- 免 : Ⅰ動詞1 （去掉;除掉） dismiss; relieve; remove 2 （避免） avoid; escape; avert 3 （免去） excuse s...
- 疫 : 名詞（瘟疫） epidemic disease; pestilence
- 原 : Ⅰ形容詞1 （最初的; 原來的） primary; original; former 2 （沒有加工的） unprocessed; raw Ⅱ動詞（原...
- 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
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After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s
通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。The results from sds - page presented that there were three female specific protein subunits with molecular weights of 123 kd, 120 kd and 91 kd, respectively. we can conclude the higher molecular compose of two subunits ; the results from two dimension electrophoresis showed the isoelectric points of two female - specific spots with molecular weight of about 120kd were 5. 5 and 5. 7. immunodiffusion reactions demonstrated that vg existed both in female fat body and hemolymph, which as vn was deposited in the ovary, while not in the male
Page電泳結果表明：麗蠅蛹集金小蜂明顯存在2條雌特異性帶-卵黃蛋白，分子量分別為181kd和136kd ； sds - page電泳分析：存在3條雌特異性帶，其分子量為123kd 、 120kd和91kd ，由此，可推定卵黃原蛋白（ vitellogenin ， vg ）和卵黃磷蛋白（ vitellin ， vn ）由2個蛋白組成，其中分子量較大的蛋白由2個亞基組成；雙向電泳結果顯示，在120kd附近有兩個特異性點，其等電點為5 . 5和5 . 7 ；雙擴散表明，麗蠅蛹集金小蜂卵黃磷蛋白的抗血清與雌隱成蟲蟲體、脂肪體、血淋巴和卵巢勻漿液均有免疫沉澱反應，而與雄蜂血淋巴無免疫反應，說明了vg與vn具有免疫同源性，是雌特異性蛋白，且由脂肪體合成。The cultivated cells showed positive reaction for - smooth muscle actin ( - sma ) antibody, and negative reaction for factor antibody with immunocytochemical assay
細胞表面有微絨毛，胞質內有豐富的細胞器，無因子小體；免疫細胞化學顯示細胞呈平滑肌肌動蛋白（ - sma ）陽性，因子相關抗原陰性。
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