煙花葉病 的英文怎麼說
中文拼音 [yānhuāyèbìng]
煙花葉病
英文
tobacco mosaic-
The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中,通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中,然後採用葉盤法,在該農桿菌的介導下將pap基因導入普通煙草中,經過卡那黴素抗性篩選,最後獲得了轉pap基因的工程煙草植株,摩擦接種煙草花葉病毒( tmv ) ,與非轉基因煙草相比,能夠推遲癥狀表現達2月之久,說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。Change of catalase activity in interaction of tobacco and cucumber mosaic virus
煙草與黃瓜花葉病毒互作中過氧化氫酶活性的變化The monopartite genome of tobacco mosaic virus is a plus-strand rna molecule of 6395 nucleotides.
煙草花葉病毒的單份基因組是一個6395個核甙的()鏈RNA分子。However, only a few host factors with clear in vivo function have been identified. by using pcr and 5 " and 3 " race, we were able to clone a homologue ( named ttom1 ) of arabdopsis thaliania host factor gene, tom1, which supports the replication of tobacco mosaic virus
根據從擬南芥中已經克隆的支持煙草花葉病毒復制的基因tom1序列設計一對特異性引物,用rt - pcr的方法獲得番茄同源基因的部分序列,然後利用5 』 race與3 』 race方法從番茄中克隆出全長ttom1基因。Studies on virus resistance of transgenic tobacco with tmv replicase gene
轉煙草花葉病毒復制酶基因煙草的病毒抗性研究The loop sequence of mb1 and mb2 were the anti sense and sense sequence ofing1, respectively the sequence of mb3 was a piece of ssrna sequence in tobacco mosaic virus, which had no analogical to human gene. mbl was the most suitable probe because mbl had the highest fluorescence enhancemen after hybridizing wtth rna extrated froin normal cell
第三章,根據一種常見的病毒煙草花葉病毒( tmv )的核酸序列設計了分子信標熒光探針,由於tmv的遺傳物質是rna ,分子信標又具有很高的特異性和靈敏度,因此感染了病毒粒子的植物葉片在經過簡單處理后,可用分子信標檢測葉片上。Cucumber mosaic virus resistant mutant from tobacco ' s anthers treated with ray through anther culture
輻射與花藥培養相結合篩選煙草抗黃瓜花葉病毒病突變體Spread characteristics of tobacco mosaic virus through clipping in tobacco floating - seedling system
煙草漂浮育苗剪葉傳播煙草花葉病毒的特點Study on tobacco germplasm screening for resistance to cmv
煙草種質資源對黃瓜花葉病毒抗性鑒定研究3. arabidopsis plants were transformed with the constructed plant binary expression vector. eighteen independent antibiotic - resistant arabidopsis transformants for dreb1c were obtained by using a vacuum infiltration method
載體全長為13kb ,有一個煙草花葉病毒35s強啟動子和nos終止序列,利用bamhi和sacl做為酶切位點,構建載體。The monopartite genome of tobacco mosaic virus is a plus - strand rna molecule of 6395 nucleotides
煙草花葉病毒的單份基因組是一個6395個核甙的( )鏈rna分子。Abstract : tobacco mosaic virus plays an very important role in the discovery of virus and several pioneers made contributions to the developmental process of virology, through which the brief history of discovery of virus can be traced
文摘:煙草花葉病病毒在病毒的發現中有著極其重要的地位,幾位病毒學研究的先驅在這方面的研究有極大的貢獻,以此為線索可以看到病毒被發現的簡要歷史。分享友人