煮沸裂解 的英文怎麼說
中文拼音 [zhǔfèilièjiě]
煮沸裂解
英文
boiling lysis-
2n methods 1 ) sample preparation the supernatant of cultures were digested with identical volume lysis buffer at 100 for 15 min. dna was isolated for use as template
二、實驗方法1 、標本處理取hcmv低傳代分離株細胞培養上清液與等量裂解液混勻后，煮沸15分鐘，提取dna ，作為擴增模板。Western blot analysis we treated 200 fertilized eggs in a series of steps including lysing them in l0ul of lysis buffer ( 50mm tris - hcl ph 7. 5, 250mm nacl, 5mm edta, imm dtt, 0. 1 % triton, 50mm sodium orthovanadate, looug / mg pmsf and tpck, 50ug / ml tlck, 1 ug / ml leupeptin pepstatin and aprotinin ), frozing them below - 70, and then thawing them at room temperature for 10 min
2 . westem印跡將處理組及對照組小鼠一細胞g2期受精卵各200個取出，轉移到ep - pendoff管中， 5000甲m離心4分鐘，棄去上清，加入ro川粉碎緩沖液，充分振蕩混勻，在液氮中經過2一3次的凍融循環，迫使卵細胞裂解，加人等量的樣品緩沖液沸水中煮5分鐘，用於westem印跡分析。
分享友人