熒光斑 的英文怎麼說
中文拼音 [yíngguāngbān]
熒光斑
英文
optic mark-
Tumor necrosis factor ( tnf ), a cytkine that exerts many pro - atherosclerotic effects in vivo, causes up - regulation of acat - 1 gene expression in human blood monocytes. by luciferase activity assay, tnf - a enhanced acat - 1 p7 promoter activity in thp - 1 monocytic cell line
細胞因子tnf -即腫瘤壞死因子大量存在於人動脈粥樣硬化斑塊中，本實驗通過熒光素酶活性和rt - pcr測定結果表明， tnf -處理人血單核細胞和單核細胞株thp - 1均增強acat - 1基因p7啟動子活性。Recombinant fpvs were selected and purified by blue plaques expressing 3 - galactosidase. the expression of foreign genes in rfpvs were confirmed by ifa. trails for evaluating protective efficacy of rfpvs against very virulent mdv or aiv challenge
純化后脂質體轉染，藍斑篩選純化得到穩定的重組病毒rfpv - ps - ha - pe / l - f ，間接免疫熒光法證實， ha基因和f基因同時得到了表達。Fluorescence power transfer function, three - dimensional point spread function ( 3d - psf ) and three - dimensional optical transfer function ( sd - otf ) for the various fluorescent wavelength of the two kinds of fluorescence confocal scanning microscopy are calculated in this paper by using fourier imaging theory. the results show that the fluorescent wavelength has influence on imaging property of confocal microscopy such as spatial cut - off frequency, resolution and 3d - otf. there is a different missing - cone in the 3 - d space of otf when the ratio of excitation wavelength to fluorescent wavelength decreases
本文在sheppard和gumin等人的理論基礎上，利用fourier光學成像理論，討論了不同熒光波長對單光子和雙光子共焦顯微鏡成像特性的影響，導出了單光子和雙光子共焦顯微鏡的熒光功率傳輸函數、三維脈沖響應函數和三維光學傳遞函數，得到了它們在不同激發波長與熒光波長比值時具體的表達式，並且通過數值計算，得到了它們的曲線圖，結果表明：隨著激發波長與熒光波長比值的增加，焦斑的橫向分佈和縱向分佈變窄，橫向解析度和縱向解析度提高，系統的成像效果變好，當激發波長與熒光波長的比值下降到一定程度時，可以看到不同程度的失錐現象。The author reviewed the detection measures of prunus necrotic ringspot virus, and related the research progression of the pathogen detection technology inside and outside. the template amplication technology include pcr assays ^ nasba and so on. the cdna and crna probe which labeled with the isotope % biotin or dig, the offset probe and the peptide probe can be applied to magnify the signal. pcr - gene scan assays and pcr agilent chip chamber combine the template amplication and the signal magnification
本文回顧了李壞死環斑病毒的檢測方法，較全面地評述了國內外病原物檢測技術研究進展：在模板的擴增有各種pcr技術、 nasba技術；在信號的放大有同位素、生物素或dig標記的cdna和crna探針，分支探針和肽核酸探針；模板擴增和信號放大相結合的有pcr -基因掃描技術、 pcr安捷倫晶元實驗室技術；模板擴增和雜交以及信號放大相結合的有pcr - elisa技術、實時熒光pcr技術、生物晶元技術。In the paper, both the size of the source and of the beam spot of a extended gaussian beam are considered and their influences on both the lateral and axial resolution of a fluorescent confocal microscopy are studied and calculated based on the power transfer function and three - dimensional pulse spread function
摘要研究一定光源孔徑、束斑的高斯光束對熒光共焦顯微鏡橫向、縱向解析度的影響，導出了熒光功率傳輸函數、三維響應函數。In order to get the soluble recombinant eo protein and inspect the protein expression status convinently, the egfp and eo gene were ligated into baculovirus transfer vector. with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna, and plaque purification in the posttransfection procedure, the pure recombinant baculovirus were harvested, which infected the sf9 cells for amplifying to generate a p - l stock. in the meantime, the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus. the pi - stock from a pure plaque was used to generate a high liter p - 2 stock, which was determined in liter as 1. 14 107pfu / ml by performing a plaque assay. when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression, the strong fluorescence was obeserved on the day 3 of postinfection
此外，為了得到可溶性重組eo蛋白並便於觀察重組蛋白的表達情況，我們將egfp基因與eo基因相連插入昆蟲桿狀病毒轉移載體中，與線性桿狀病毒dna共轉染sf9細胞后通過噬斑純化得到純的重組桿狀病毒，將其感染sf9細胞制備p1種子液，同時用熒光顯微鏡觀察綠色熒光蛋白的表達情況剔除表達效果差的重組桿狀病毒。再用p1種子液感染sf9細胞制備高效價的p2種子液。通過病毒液的梯度稀釋和噬斑測定，確定p2種子液的病毒滴度達1 . 14 10 ~ 7pfu ml 。Confocal laser scanning microscopy confirmed the co - localization of fluorescent immunomicelles and macrophages in the atherosclerotic plaques, and the leels correlated with the mri findings
激光共聚焦顯微鏡掃描證實了，熒光免疫微粒和巨噬細胞共同積聚在動脈硬化斑塊中，並且水平與mri的發現相關。分享友人