熒光鏡檢法 的英文怎麼說

中文拼音 [yíngguāngjìngjiǎnfǎ]
熒光鏡檢法 英文
fluorescence microscopy
  • 熒 : 形容詞[書面語]1. （光亮微弱的樣子） glimmering 2. （眼光迷亂; 疑惑） dazzled; perplexed
  • 光 : Ⅰ名詞1 （照耀在物體上、使人能看見物體的一種物質） light; ray 2 （景物） scenery 3 （光彩; 榮譽） ...
  • 鏡 : Ⅰ名詞1 （鏡子） looking glass; mirror 2 （幫助視力或做光學實驗的器具） lens; glass 3 （姓氏） a s...
  • 檢 : Ⅰ動詞1 （查） check up; inspect; examine 2 （約束; 檢點） restrain oneself; be careful in one s c...
  • 法 : Ⅰ名詞1 （由國家制定或認可的行為規則的總稱） law 2 （方法; 方式） way; method; mode; means 3 （標...
  1. The epitaxial growths of ingaas / gaas / algaas fundamental material and the fabrication of 45 - deflector are extensively studied in our work. some measuring methods are used to evaluate the growth quality of our grown structure by pl, cv, x - ray double crystal diffraction, sem etc. property analysis are provided for it

    利用高能電子衍射、電化學c - v 、掃描電鏡（ sem ） 、 x射線雙晶衍射儀、光熒光譜儀（ pl ） 、原子力顯微鏡等多種方法對制備的器件進行了檢測，同時對實驗結果進行了必要的分析。
  2. Methods according to theory of specific binding of antigen and antibody, at first the anti - a monoclonal antibody ( ma ) and anti - bma were labeled with the fluorescent, then fluorescent - labeled antibodies ( fla ) were bound with corresponding biological material ( such as bloodstain ) in the optimum condition, finally the abo blood type of bloodstain was determined under microscope fluorescent

    方法根據抗原抗體特異性結合的原理，首先對抗a 、抗b單克隆抗體進行熒光標記，然後使熒光標記抗體與相應抗原（血痕）在最佳條件下結合，最後熒光顯微鏡鏡檢，判定血痕的血型。
  3. First, glass slides having been rinsed will be treated with nh3h2o, aminosilane and aldehyde. second, the quality of pretreatment surface of glass slides can be tested through methods of fluorescence and afm microscope. in the end, the characteristic of probe immobile ratio for oligonucleotide on glass surface is obtained through researching the internal relation of these two methods

    實驗選用表面平整的德國玻片，將清洗好的玻片分別進行羥基化、氨基化、醛基化，採用熒光法和原子力顯微鏡法分別檢測玻片表面預處理質量，研究兩種檢測方法之間的內在聯系，從而確定表徵玻片表面寡核苷酸探針固定率的方法。
  4. According to the gene sequence and secondary structure of hcv ns5b, we design the sirnas targeting ns5b gene following with the requirement for sirnas design from tuschl et. al and synthesize it from dharmacon company ; hepg2 cell stably expressing ns5b - egfp protein was trasfected by synthesized sirnas with electroportion, the non - transfected cell and non - specific sirnas transfected cell are c onsidered as control group ; inhibitory effect of sirnas was investigated by fluorescence microscope with dapi dyeing and by semi - quantitative rt - pcr

    然後根據dsrna設計原則，結合nssb基因的序列特徵，藉助生物信息學軟體設計了針對nssb基因的sirnas ，並交由公司化學合成；電穿孔法轉染上述穩定轉染的細胞克隆，同時分別以非特異的sirnas轉染組和空白轉染組為對照， dapi染色后通過熒光顯微鏡和內標化rtpcr檢測，初步證實了化學合成的sirnas可以特異阻斷nssb基因的表達。
  5. In this dissertation, the plasmids containing 5s promoter were transfected into cho cells and the transcription sites of rna polymerase and its transcripts were detected by fluorescence in situ hybridization to dna and rna, respectively

    本實驗以中國倉鼠卵巢細胞（ cho ）為實驗材料，利用基因轉染、熒光原位雜交並結合激光共聚焦顯微鏡觀察的方法，在dna和rna水平上分別對rna聚合酶的轉錄位點和轉錄子的分佈進行了檢測。
  6. Aqueous fluid volume and [ c1 ~ j were assayed in samples withdrawn by micropipettes. intraocular pressure ( top ), pressure - dependent outflow, and anterior chamber compliance were determined from pressure measurements in response to pulsed and continuous fluid infusions into the anterior chamber using micropipettes. result : in wildtype mice ( gdi genetic background, age 4 - 6 weeks ), iop was 16. 0 ? 0. 4 mmhg, aqueous fluid volume was 7. 2 ? 0. 3 ul, aqueous fluid production was 3. 6 ? 0. 2 ul / hr, aqueous fluid outflow was 0. 36 ? 0. 06 ul / hr / mmhg, and anterior chamber compliance was 0. 036 ? 0. 006 ul / mmhg ( mean ? se, 8 - 10 eyes )

    實驗方法包括：將熒光物質用電離子滲透的方法穿透角膜導入活體小鼠的前房中，然後應用共聚焦顯微鏡根據熒光強度變化測量房水生成率；通過顯微注射針吸取房水檢測房水容積和氯離子濃度；顯微玻璃管刺入前房測量眼內壓，並將生理鹽水分別以連續和脈沖兩種方式注入前房，測量房水間隙的順應性和房水排出與眼內壓的相關性。
  7. This study we acquired the coding region of hcv ns5b gene by pcr of hcv full length genome and construct the recombinant plasmid pegep n3 - ns5b ; with the different concentration of g418 in the culture medium, we think the selection concentration of g418 for hepg2 cell is 800 g / ml ; the recombinant plasmid was transfected into hepg2 cell by lipofectamine2000 cells containing stable transformants were selected by the ability of resistance to g418 and isolated with the limited dilution. the stably transfected cell line expressing ns5b - egfp fusion protein was obtained by the detection under fluorescence microscope and rt - pcr

    本研究首先從hcv基因組中擴增出nssb基因，構建了nssb基因與報告分子egfp （增強型綠色熒光蛋白）基因的融合基因真核表達質粒pegfpn30 ；通過含有不同g418濃度梯度的培養液培養hepgz細胞，確定了篩選用g4181作濃度為800pg ml ；利用脂質體法將該重組質粒轉染hepgz細胞，經過有限稀釋法和g4壓力選擇，應用熒光顯微鏡和rtpcr檢測，獲得可穩定表達nssbegfp融合蛋白的hepgz細胞克隆。
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