Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫
熱穩定性天然弱毒b _ ( 95 )株接種spf雞胚繁殖病毒,經處理后提取病毒的基因組rna ,參考國內外發表的ndv融合蛋白基因序列,設計一對特異性引物,經反轉錄
聚合酶鏈式反應( rt - pcr )擴增出約1700bp大小的特異性片段,將此片段回收純化后,利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中,再轉化大腸桿菌jm109感受態細胞,轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
We have synthesized the imprinted polymers using phenacetin, nicotine, paracematol and aminopyrine as the template molecules respectively and deduced the response model of the piezoelectric bio - mimetic sensors
我們合成了非那西丁、尼古丁、撲
熱息痛和氨基比林四種模板分子的印記
聚合物,得出了壓電模擬生物傳感器的響應模型。
Sir thomas exerted himself so far as to speak a few words of calm approbation in reply to an eager appeal of mr. yates, as to the happiness of the arrangement.
耶茨先生
熱切地請求參加他們合家的快樂
聚會,托馬斯爵士勉為其難地冷冷說了幾句表示同意。
This test chooses general industrial vc / vac copolymer as raw material, various supplement material additives are also the common conventional avirulent additive on industry ; the plastic material processing routine is also the universal method to make the thermoplastic plate of low temperature of vc / vac copolymer that accords with medical requirement that its softening temperature is 55
本試驗選用氯醋共
聚樹脂為主要原材料,所用輔料添加劑也是工業上常見的無毒性常規添加劑;採用常規的塑料加工成型方法及設備製造出軟化點為55的、符合醫用要求的低溫
熱塑性氯醋共
聚樹脂板材。
Separately, tma can be used as the cross linking agent to make the polyester resin and epoxy resin for the making of thermoset powder coatings, binding agent, and intermedium of dyestuff, medicine, and resin. moreover, tma can be used to make polyester imido coatings which care special insulated paints for motors
另外,偏酐還可以作
熱固性粉末塗料
聚脂樹脂和環氧樹脂的交聯劑,可用於分散性氨基甲酸乙脂
聚脂中,使其在環境溫度為70時,性質穩定,並具有高光澤度快乾耐磨等多種特性。
There are positive opinions and negative ones about what weather christmas will harms the traditional culture of china ; look around the bonhomie in shanghai, however, and the excitement of the public of welcoming to christmas day, it just let everyone find a chance to meet together, and don ' t take the culture matter too serious
到底過聖誕節,是不是會侵害傳統的中國文化,正反兩面說法是公說公有理,婆說婆有理;不過看看上海街頭
熱鬧的氣氛,以及民眾等著迎接假期的興奮心情;其實,過節嘛,不就是大家找個機會
聚在一起聯絡感情,好像沒有這麼嚴重。
The water of the heat preservation water tank is just decalescence, heat accumulation and thermolysis the water of the water tank is invariablenes
保溫水箱里的水吸
熱性良好,加之保溫水箱里的水在
熱聚集和
熱吸收方面是永恆的。
The effect of oxidative polymerization of pitch on properties of micro - pore carbon
煤瀝青
熱聚合過程對微孔炭特性的影響
Standard test methods for rubber property - shrinkage of raw and compounded hot - polymerized styrene - butadiene rubber
橡膠性能的標準試驗方法.
熱聚合丁苯橡膠生膠和復合橡膠的收縮性
Firstly, a situ - polymerization method as the preparation process of gplb is introduced and pmma as the polymer matrix of the gel electrolyte is selected. the thermal polymerization process of the gel electrolyte is also optimized
首先,採用了現場
聚合法制備凝膠
聚合物鋰離子電池的工藝,根據該制備工藝選擇pmma基作為該凝膠
聚合物電解質的
聚合物體系,然後對凝膠的
熱聚合工藝進行了優化,確定了最佳的
熱聚合工藝。
Focusing on the preparation process of lithium battery, thermal polymerization process of the gpe and optimizing the component of the gel electrolyte, the paper intends to resolve some of the key problems mentioned above and to prepare a gplb with good performance
本論文正是從目前凝膠
聚合物鋰離子電池存在的這些問題出發,從鋰離子電池的制備工藝和凝膠的
熱聚合工藝研究著手,對gpe的組份進行了改進和優化,制備了性能良好的凝膠
聚合物鋰離子電池。
A new method of manufacturing hot - fill pet bottles
製造耐
熱聚酯瓶的新方法
( 5 ). glucose oxidase glass micro - pearls, the biology sensitive materials was prepared based on oxygen sensitive materials made by thermo - polymerization method, and could be utilized to detected the concentration of glucose in solution
( 5 )氧敏感材料在光纖葡萄糖傳感器中的應用:採用
熱聚法制備氧敏感材料並在此基礎上制備了生物敏感材料? ?葡萄糖氧化酶玻璃微珠。
If the protostar is slightly more massive than a planet, but not massive enough to burn its nuclear fuels, it becomes a
如果一顆原恆星的質量只是比行星大少許,它的中心便不能產生
熱聚變,成為一顆昏暗而難以被發現的
With the exception of fusion and iter, the overall european effort is paltry, and is mainly based in universities ? which are good at identifying ideas, but are not equipped to take them forward into commercial application
熱聚變和國際
熱核
聚變能源研究項目作為唯一的例外之外,整個歐洲在能源研究開發方面的努力主要建立在許多大學的基礎上?它們在鑒別想法方面不錯,但是缺乏足夠資源能夠將好的想法推進到商業應用。
600 v grade heat - resistant polyvinyl chloride insulated wires
600 v等級的耐
熱聚氯乙烯絕緣電線
The case is made of heat - resisting polyurethane with foaming treatment, which endows the case an excellent heat - insulating property, and the outer cover keeps a relative constant temperature and long insulation without distortion in the operation, it is energy saving and greatly prolongs the lifespan of the machine
採用耐熱聚胺脂整體發泡工藝,令箱體隔熱性能優良,外殼升溫小,保溫時間長,整體無變形,既節能環保,又相應地延長了整機的使用年限。