牛度生長素 的英文怎麼說

中文拼音 [niúdùshēngzhǎngsù]
牛度生長素 英文
somidobove
  • 牛 : 名詞1. （哺乳動物） cattle; ox 2. （姓氏） a surname
  • 度 : 度動詞[書面語] （推測; 估計） surmise; estimate
  • 生 : Ⅰ動詞1 （生育; 生殖） give birth to; bear 2 （出生） be born 3 （生長） grow 4 （生存; 活） live;...
  • 長 : 長Ⅰ形容詞1 （年紀較大） older; elder; senior 2 （排行最大） eldest; oldest Ⅱ名詞（領導人） chief;...
  • 素 : Ⅰ形容詞1 （本色; 白色） white 2 （顏色單純） plain; simple; quiet 3 （本來的; 原有的） native Ⅱ名...
  • 生長 : grow; grow up; ascent; merisis; build up; auxesis; increment; overgrowth; gain; burgeon; bourgeon...
  1. Rlean meat percentage is one of the most important economic traints in pig breeding programs. myostatin is a negative regulator of skeletal muscle growth. null or low activity of myostatin, individual muscle of mutant amimals would show a large and widespread increase in skeletal mass. myostatin null animals have significantly larger diameter or more quantity of fiber skeletal muscle. the phenotype was termed double muscling. in order to probe the relation between myostatin and high lean meat rate and plump - hipped trait, we sythesized the c ' 80 amino acids coding sequence of porcine myostatin and costructed the cloning and expressing vector of it

    肌生成抑制素（ myostatin ，即mstn ）是近幾年來（ mcpherrona . c等， 1997 ）發現的骨骼肌生長的負調控因子，它主要在骨骼肌中表達。其活性的喪失，會引起動物肌肉的過度發育，肌纖維直徑變大或肌纖維數增加，表現為雙肌癥狀。肌生成抑制素研究的突破將對豬、肉雞、肉牛等畜禽生產性能的提高具有特別重要的意義。
  2. Then, 5. 5kb thrombiotin gene was amplified with the same technique from the genome of a baby ' s blood, which included the begining part of intronl to the teminator. in addition, 6. 0kb and 1. 8kb homlogous arms were also amplified from a cow with high yield. the 6. 0kb homologous arm contains the promotor, extron 1, extron2, extron3 and intron 1, intron2 and part of the intron3 fragment, while the 1. 8kb homologous right arms contains exon13, exon14 and part of intron 13, the whole intron14 and part intron 14 of asl - casein gene of bovine

    通過長片段pcr從高產奶牛的基因組中獲得了打靶所需的長、短同源臂序列，長度分別為6 . 0kb和1 . 8kb ，位於s1 -酪蛋白基因的5上游區到第三內含子和十二到十四內含子；從綿羊全血基因組克隆得到了綿羊的-酪蛋白基因啟動子區到第二內含子區4 . 1kb的5調控序列；利用同對引物克隆得到了水牛的同基因序列；從廣西當地一嬰兒臍血基因組中通過獲得了人血小板生成素基因，位於第1內含子到終止子後部分的序列，長達5 . 5kb 。
  3. Main works and their important results are showed as following. firstly, the cdna encoding bullfrog gh is amplified by use of the total rna, extracted from adult bullfrog ' s pituitary, as the template, pi ( 5 ' - cggatcc atg gct tca ggg tta ggc ) and p2 ( 5 ' - cgaattc tta aaa ggt gca gtt gct ) as the primer pair and one particular cdna product, 660bp, was obtained after the rt - pcr - the product was purified by using dna agarose gel extraction method. after the purified cdna and pmd18 - t vector were ligated at 16 over night, the ligation products were transformed into e. coli strain dh5a and then the transformants were screened by clone pcr method

    首先，以成體牛蛙的腦垂體總rna為模板，進行rt ? pcr擴增得到約660bp的特異性pcr產物帶，切下瓊脂糖凝膠中的特異產物帶進行cdna膠回收，將cdna與pmd18 ? t載體進行t ? a克隆連接並轉化到dh5 e . coli后，進行菌落pcr 、質粒酶切鑒定，篩選出陽性菌株，測序結果經blast分析，與已報道的牛蛙生長激素基因高度同源，證實此陽性克隆為牛蛙生長激素基因轉化子，命名為pbfgh 。
分享友人
分享到 Line

分享到臉書