特異位點 的英文怎麼說

中文拼音 [tèyìwèidiǎn]
特異位點 英文
specific site
  • 特 : Ⅰ形容詞（特殊; 超出一般） particular; special; exceptional; unusual Ⅱ副詞1 （特別） especially; v...
  • 異 : 形容詞1 （有分別; 不相同） different 2 （奇異; 特別） strange; unusual; extraordinary 3 （另外的;...
  • 位 : Ⅰ名詞1 （所在或所佔的地方） place; location 2 （職位; 地位） position; post; status 3 （特指皇帝...
  • 點 : Ⅰ名詞1 （液體的小滴） drop （of liquid） 2 （細小的痕跡） spot; dot; speck 3 （漢字的筆畫「、」）...
  1. Some tendency of tn5gusa5 transposition were found that all preferred sites of tn5gusa5 in xcc 8004 genomic dna are in at - rich regions ; target sequences of tn5gusa5 have some features that the probabilities of guanine and cytosine are high respectively at the head and tail base of target sequence ; the level of gene transcription does not influence insertion density of tn5gusa5 significantly

    結果表明， tn5gusa5插入位點有一定的規律性： tn5gusa5在xcc8004基因組上傾向于插入低gc含量（ 50左右的區域插入密度最高）區段；插入位點的靶序列有一定的特異性，在靶序列的首位鳥嘌呤出現的幾率高，而在靶序列的末位胞嘧啶出現幾率高； tn5gusa5的插入密度與該區段基因的轉錄水平無明顯關系。
  2. The cbd tag of the fusion protein was cut by site - specific protease enterokinase at starting met of the target protein lt 27. it was released from the cbd fusion tag efficiently

    利用融合蛋白中目的蛋白lt 27與cbdtag接頭處的腸激酶特異性識別位點，用腸激酶處理粗純化的融合蛋白，可將卜
  3. The second part, a renewable piezoelectric immunosensor is developed for the antibody of schistosoma - japonicum ( sjab ). after incubating 32 kd molecular antigens of schistosoma japonicum ( sjag ) on the qcm by applying the immobilization above, the nonspecific sites on the immunosensor are sealed by using bsa and nrs together. the immunosensor can detect the sjab with the linear range of 0. 54 ~ 32. 50 ug / ml

    以感染兔血清為檢測對象，採用聚電解質吸附固定法，將日本血吸蟲分子抗原（ siag32kd ）固定於石英晶振表面，再以牛血清白蛋白（ bsa ）和正常兔血清（ nrs ）聯合封閉晶振上非特異性活性位點，可在0 . 54 32 . 50ug ml范圍內檢測感染兔血清中日本血吸蟲抗體。
  4. Transgenic integration site detection is the efficient method to identify whether the transgenic crops are approved to import or export. some quantitative detection methods were reported in 2000 and 2001, and that is about bt11 and mon810 corn. real - time 5 " - nuclease pcr had been previously used successfully to determine quantitatively roundup ready ( rr ) soy and btl76 maize in food

    轉基因整合位點檢測是鑒定轉基因品種是否為批準進口轉基因作物的有效的特異性方法，國外在2000年和2001年已開展了轉基因玉米bt11 、 mon810兩個品種整合位點的特異性檢測方法研究。
  5. The result showed that the homology rate of pila gene among the 5 avian pathogenic e. coli strains tested and one human e. coli were from 89. 8 % to 91. 1 %, and the homology rate of amino acid were from 88. 5 % to 91. 8 %. the homology rate of pila gene sequence among 5 avian pathogenic e. coli strains tested and avian pathogenic e. coli reported ( serotype o1, o2, o78 ) were from 87. 8 % to 90. 2 %, and the homology rate of amino acid were from 84. 6 % to 91. 2 %. there had homology in avian pathogenic e. coli. there had some common antigen side in type 1 pili of avian pathogenic e. coli

    結果表明：運用msha法檢測1型菌毛的檢出率為80 （ 36 45 ） ， pcr法的檢出率為95 . 5 （ 43 45 ） ， pcr方法用於1型菌毛的檢測比msha更加敏感、快速、特異性強；選擇5株優勢血清型雞源致病性大腸桿菌代表株（ o _ （ 89 ） ， o _ （ 119 ） ， o _ （ 141 ） ， o _ （ 127 ） ）的1型菌毛pila基因的pcr擴增片段經純化后，分別定向克隆到puc18質粒的多克隆位點，構建了含有目的基因片段的克隆質粒，並轉化到dh5株大腸桿菌載體菌中，篩選獲得陽性克隆菌株。
  6. In some cases the observed segregation ratios even allowed us to clearly determine that m. xiaojinesis is a autotetraploid plants, at least is a isosyndetic allotetraploid. these were the cases of 32 m. xiaojinesis - specific markers and 50 markers present in both parents, which fit 5 : 1 and 11 : 1 ratio respectively. since these cases happened were owing to m. xiaojinesis ' s duplex loci

    50個雙親共有標記和32個小金海棠特有標記分別適合11 ： 1和5 ： 1的分離系數，由這兩個系數可以推測，小金海棠可能是同源四倍體，至少是同源異源四倍體，因這兩個系數都表明其產生是源於小金海棠的雙式位點。
  7. Protein engineering and site directed mutagenesis have been used to change the active site and alter the substrate specificity of various hydroxynitrile lyases

    研究人員已經利用蛋白質工程和定點突變技術來改變各種醇腈酶的活性位點和底物特異性等。
  8. Application of microsatellite dna polymorphisms and dna fingerprints to inbred strain mice and rats to screen the exact, dependable, particular genetic monitoring marker method of laboratory animal, the author had studied the application of microsatellite dna polymorphisms and dna fingerprints to inbred strain mice and rats, and compared the two methods with the biochemical marker enzyme method. the study had established the foundation of the molecular genetic monitoring marker method of laboratory animal

    本文通過對dna指紋技術和pcr擴增微衛星dna技術在近交系大、小鼠遺傳檢測中的應用研究，並與生化位點標記分析法進行比較，旨在篩選出具有精確、可靠、特異性好的實驗動物遺傳檢測方法，為建立分子生物學實驗動物遺傳質量監測技術和標準奠定基礎。
  9. The env protein deduced from env gene encodes the hydrophilic surface protein ( su ) and the hydrophobic transmembrane domain ( tm ) that determine the specific interaction between virus particles and cell surface receptors during retroviral entry. the su of retroviruses is a highly variable genetic element, containing receptor binding sites and major antigenic determinants. exjsrv - specific dna probes were derived. by using these dna probes in tissue hybridization. we successfully identified jsrv mrna expression and proviruses dna in sheep lung tissues infected with jsrv and control group has no postive signals, validating the use of exogenous virus - specific dna probes in the analysis of oncogenic proviral integration sites and identification of integrated exogenous proviral sequences

    用地高辛隨機引物法標記exjsrv特異的env片段，制備探針，原位雜交檢測spa肺組織中的rna及前病毒dna ，結果表明spa患羊肺組織內有jsrvenv基因mrna的表達，同時也檢測到了前病毒dna ，而相應的陰性對照卻無陽性信號，證實外源性病毒特異的dna探針在致瘤性前病毒的整合位點和整合的外源性前病毒的檢測中具有可信度。
  10. These results indicate that the alteration of cell proliferation and dna synthesis caused by different gnt - v cdna transfection may at least partly result from the modification of n - glycan structure and function of egfr. it seems that the increased 1, 6 glcnac branch on the n - glycans of egfr may benefit to its binding with egf and the resulting tyrosine auto - phosphorylatio n, while the decrease of this branch may prevent these processes

    用特異性抗體結合westemblot結果發現，正義或反義gnt一vcdna的轉染並不引起pkb 、 p44 / 42mapk和mek蛋白質表達的變化，而gntv一s / h 」 21細胞pkbt308 、 5473位點磷酸化和免疫沉澱pkb的酪氨酸磷酸化以及以gsk召a /日磷酸化為指標的pkb的活性都較mock細胞增加， gntv一as / h7721細胞中這些指標的變化則相反。
  11. Telomerase is a ribonucleoprotein complex ( rnp ) composed by its rna component and protein subunits. telomerase can synthesize telomeric dna onto chromosomal ends using its own rna component as a template, elongate the length of telomere, increase cell life and even induce cell immortalization

    端粒酶（ telomerase ）是由端粒酶rna和蛋白質組成的一種核糖核蛋白復合物（ rnp ） 。端粒酶含有引物特異識別位點，能以自身rna為模板，逆轉錄合成端粒dna並加到染色體末端，使端粒延長，從而延長細胞的壽命甚至使其永生化。
  12. The best bet at the moment is probably that the phage integrase method can be used by getting a better hold of how its site - specificity works and therefore being able to design changes to it rather than using the very weak technique of in vitro evolution - - but this will need a lot of hard work

    目前大可一賭的是：嗜菌體整合酶方法也許可以利用：較好地拿捏位點特異性使之起作用，而因此能設計改變嗜菌體整合酶，而不是利用體外進化的很不可靠的技術?但這需要做大量艱苦的工作。
  13. But polyadenylation in bacteria needs no specific consensus sequence or there is no such sequence signals found. the sites of polyadenylation of bacterial mrna are diverse, including the 3 ' ends of primary transcripts, the sites of endonucleolytic processing in the 3 ' untranslatd and intercistronic regions, and sites within the coding regions of mrna degradation products

    細菌mrna多聚腺苷酸化的位點多種多樣，包括初級轉錄產物的3 』末端， 3 』端非翻譯區和順反子間區的內切酶加工位點及mrna降解產物的編碼區內，其腺苷酸化相對無特異性、無選擇性。
  14. The results indicate that the nucleotide sequences and deduced amino acid sequences of all the guangxi isolates in the signal peptides were highly homologous, but lowly homologous with other reference strains. the amino acid composes and arrangement of all guangxi isolates at the cleavage site has the typical pattern of ndv virulent strains, and is identical with the facts in the field cases. all the guangxi isolates are classified into genotype vii of apmv - 1, the same genotype dominated in china and other areas in recent years

    結果發現，廣西分離株之間在信號肚的核旮酸和氨基酸同源性很高，而與其它參考株差異較大；廣西分離株在裂解位點的氨基酸組成和排列均符合強毒株的特徵，並與毒株在臨床上的致病情況相符；根據apmvlf基因第47位第420位核苦酸序列所繪制的系譜樹吵ylogenetictree ）來看，廠西雞和鵝分離株都歸屬于基因型vll 。
  15. Besides the immunological functions, mhc genes also play important roles in many other respects. the polymorphism of mhc genes, especially of mhc class ii genes, is the most essential property. moreover, mhc genes are usually used to analyze the genetic structure and genetic variation in conservation genetics

    Mhc除了具有免疫功能外，還在其它許多方面起作用： mhc基因的多態性是最受關注的特徵，尤其是類基因，因此可將mhc位點作為一種遺傳標記，用於種群遺傳結構和變異性的研究，這對於一些瀕危物種的遺傳保護起到重要的作用。
  16. The gene of amoa in ammonia - oxidizing encodes the active - site polypeptide of ammonia monooxygenase which catalyzes the oxidation of ammonia to hydroxylamine. we designed a pair of primers special for the amoa gene by comparing the known amoa gene sequences and used pcr to amplify the amoa gene fragments

    Amoa基因是編碼氨單加氧酶活性多肽位點基因，我們通過引物篩選合成了對氨氧化細菌amoa基因特異結合的引物序列，利用pcr技術對活性污泥中的amoa基因片段進行特異擴增，得到的dna片段大約為490bp 。
  17. As well as in eukaryocyte ( hepg2 and cos - 7 ), then detect their antigenity as a basis study and explore of the choice of immunogen for preventive and therapeutic vaccines of hepatitis b. methods : the gene fragments coding 152aa ( si ) and 124aa ( s2 ) of the carboxyl terminus of hbsag were amplified by pcr from plasmid pecob6 with a pair of primers containing different endonuclease sites and were cloned into multiple cloning sites of plasmid pbks ( + )

    為乙型肝炎的預防和治療性疫苗免疫原的選擇進行初步的研究和探討。方法：本研究利用聚合酶鏈反應（ pcr ） ，通過設計帶有不同酶切位點的一對引物，從質粒pecob6特異性擴增hbsag蛋白羧基末端152個氨基酸（ s1 ）和124個氨基酸（ s2 ）的基因片段，分別將二者克隆到質粒pbks （ + ）的多克隆位點，篩選重組克隆。
  18. In the part i, the sequence of the cloned promoter osg6b " was first analyzed. osg6b " had a whole length of 1688 bp and 98 % homology to known sequence of promoter osg6b. its transcriptional start point, tata boxes and the consensus sequences " tgtgg " conserved usually in anther - specific promoters were identical to those of reported osg6b

    第一部分：對已克隆的啟動子osg6b 』進行的序列分析表明， osg6b 』全長1688bp ，與報道的osg6b有98的同源性，兩者在轉錄起始位點、 tatabox及其它花藥特異性啟動子共有的保守序列tgtgg完全相同。
  19. Surface plasmon resonance immunosensor is a relatively new immunoassay technique and has been receiving more and more attention in recent years. however, a major disadvantage of spr for bioanalytical applications is that low concentration or low molecular mass analytes could not be detected directly. therefore, it is a challenging task to develop strategies for improving the detection limit sensitivity of spr. in this paper, authors present a novel strategy for improving the sensitivity of spr immunosensing using streptavidinbiotinylated antibody complex. it is proven that the amplification strategy causes a dramatic improvement of the detection sensitivity. this amplification strategy is based on the construction of a molecular complex between streptavidin and biotin labeled protein. the complex can be formed in a crosslinking network of molecules so that the amplification of response signal will be realized due to the big molecular size of complex

    將鏈霉親和素-生物素系統用於表面等離子體共振免疫傳感的信號放大，實時檢測了人免疫球蛋白g higg的蛋白濃度。發生免疫反應的傳感片和生物素化抗體反應后，傳感片表面的一層生物素分子隨后與鏈霉親和素-生物素化抗體復合物中的鏈霉親和素的活性位點發生親和反應，從而使傳感片表面特異健合的物質質量顯著增加，大大提高了免疫檢測的靈敏度和檢測限。免疫反應經放大后，可檢測0 . 00510g ml濃度區間內的higg 。
  20. In n terminal, 3 conserved sequences, wxixgmxgxgkttla, l ( i / v ) ( v / l ) lddv ( w / d ) and sriixttrd xxv are in term of p - loop, kinase 2 and kinase 3a. those are highly identical to the homologous of human ( apaf - 1 ) and namenode ( ced - 4 ). it revealed that tm - 22 encodes a membrane protein with a receptor structure and kinase property and may function in ion flow, phosphorylation and proteins interaction

    Tm一夕基因的編碼蛋白在該區與tm一2有32個氨基酸的差異，主要集中在第10 、 11和12個亮氨酸重復序列中，與tm一2有兩個氨基酸的差異，僅僅是位於第12個亮氨酸重復序列中的767和769兩個位點，這表明:第一， lrr是tm一22基因和tm一2基因編碼蛋白對病毒識別的特異區段;第二， 767和769兩個位點的差異氨基酸是tm一22基因和tm一2基因編碼蛋白對病毒識別的特異位點。
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