生化培養基 的英文怎麼說
中文拼音 [shēnghuàpéiyǎngjī]
生化培養基
英文
biochemical medium-
In this study, the stem segments of new shoot with axillary buds of well - growth tetraploid black locust trees were used as explants. the effects of different basic mediums, different hormone kinds and their concentrations ratios, different sucrose concentrations on calli induction, buds differentiation and rooting in the process of establishment of high frequency regeneration system of tetraploid black locust were studied. on the base of high frequency regeneration system, the effects of various factors on transformation efficiency of badh mediated by agrobacterium tumefaciens were discussed in the light of gus histochemical assays
本實驗首先以生長良好的四倍體刺槐優株上當年生新梢的帶腋芽莖段為外植體，研究了在四倍體刺槐高頻再生體系的建立過程中不同基本培養基、不同激素濃度及其配比、不同蔗糖濃度對愈傷組織的誘導、芽的分化及生根的影響；然後在得到高頻再生體系的基礎上，通過農桿菌介導法轉化甜菜堿醛脫氫酶（ badh ）基因，以gus染色組織分析為依據探討了影響轉化效率的各種因素，建立了高效、可重復的基因轉化體系，為四倍體刺槐目的基因的導入打下了基礎。The results showed that : adding tryptone, soy peptone. beef extract, com extract and cys - hcl to jaj could obviously promote the growth of blm and bbm ; by the orthogonal experiment of three elements and three levels, a satisfying jaj compound medium was acquired which included corn extract ( 0. 3 % ), soy peptone ( 0. 05 % ) and cys - hcl ( 0. 025 % ). nextly, after establishing a selective bifidobacterium medium, the effects of jaj on the growth of bifidobacteria in vivo were studied, using healthy mouse of kunming species as experimental animal
研究了以菊芋汁為主要原料的雙歧桿菌培養基，大量試驗結果表明，在菊芋汁中添加胰蛋白腖、牛肉膏、大豆蛋白腖、玉米漿和半胱氨酸鹽酸鹽等成分，對雙歧桿菌有明顯的促進生長作用；利用大豆蛋白腖、玉米漿和半胱氨酸鹽酸鹽設計了三因素三水平的正交試驗，確定了菊芋汁復合培養基的優化配方：菊芋汁+ 0 . 3玉米漿+ 0 . 05大豆蛋白腖+ 0 . 025半胱氨酸鹽酸鹽。The lipase b from candida antarctica ( cal - b ) displays high enantioselectivity on a broad range of substrates, making it an accepted biocatalyst for asymmetric organic chemistry
摘要從南極假絲酵母中提取的脂肪酶b ，具有較高的對應異構體選擇性，廣泛應用在培養基中，作為不對稱有機化學的生物催化劑。Explants were then transferred onto the selection medium containing 500mg / l carbenicillin and 150mg / l kanamycin and incubated at 25, 16 / 8h light / dark cycle. leaves of adventitious shoots were detected by gus staining
Sin旮16ea ）上暗培養3天後，移入加有抗生素150mg ikan和500mg幾cr的誘芽培養基上誘導不定芽的分化。Explants were then transferred onto the selection medium containing 500mg / l carbenicillin and loomg / l kanamycin and incubated at 25, 16 / 8h light / dark cycle. small leaves of adventitious shoots differentiated from explants were cut and dipped into gus staining solution. positive shoots, gus tinted, were induced to root
分化出的不定芽切下半張葉片進行gus染色，呈陽性的植株從外植體上切下，在生根培養基（ ms 0刀sing lnaa 50mg幾kan ）中誘導根的形成。2. according to the effect of combination of different hormone concentration in the medium on callus formation and shoot induction of tomato cotvledons, we defined mso + 2. 0mg / l ba + - 0. 2mg / l iaa as optimum differential medium
根據番茄子葉外植體在加有不同激素濃度配比的培養基上愈傷組織分化和芽再生的情況，確定最佳分化培養基為ms _ 0 + 2 . 0mg / lba + 0 . 2mg / liaa ； 3According to these problems, we adopt to the method of mending material, optimize to fermentation media and partly ferment condition. finally, we excogitate a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified. with the plasmid pbv220 - ifnr, pbv220 - hgfa, pbv220 - hgfb, pbv220 - hpk5 that expresses serve as the model, adopting the biostat - c15l of b. braun company, utilize the method of mending material to ferment, through optimization fermentation media and optimization partly ferment condition ( ventilate quantity, stir speed, mend material speed ), eventually establishment a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified
以我室構建並穩定表達的重組質粒pbv220 - - ifn 、 pbv220 - hgf 、 pbv220 - hgf 、 pbv220 - hpk5為模型，分別從不同的表達宿主菌中篩選出一種適合大規模生產的菌種bl21 （ de3 ） ，該工程菌株連續傳代100代表達質粒不丟失，表達量穩定；採用b . braun公司的biostat - c15l自控發酵罐，運用分批補料技術分別進行四種工程菌的高密度發酵，通過優化工程菌發酵的培養基配方及優化部分發酵條件（通氣量、攪拌速度、補料速度） ，最終建立一種適于目的基因高效表達的高密度發酵工藝模式。Eng. ) preparation of media, culture of bacteria, isolation and purification of bacteria, preservation of bacterial strain, gram stain and observation of bacterial strain, biochemical test, growth curve, preparation and analysis of bacterial dna
中）培養基的制備，菌株的培養，菌株的分離及純化，劃線分離法，及連續稀釋法，菌株的保存，菌株的格蘭氏染色法，菌株生化反應的測試，菌株生長曲線的測定，菌株的染色體dna之制備及分析。Cotyledon and hypocotyl ' s rate and quamity are the most among these explams, and callus can be obtained in 10 days by cotyledon and hypocotyl. reversely it is difficult to indue callus with root, and the callus from root is lnde and easy to become browning. the calius obtained from leaf grows very slow and does not become browning uniill in 2 or 3 months
銀杏的不同器官和組織都能夠誘導出愈傷組織來，其中，子葉和胚軸10d左右全部愈傷化，誘導速度和誘導率均最高，根則很難誘導，愈傷組織很少，褐化很快；葉片誘導的愈傷組織，生長慢，褐化也慢，在培養基上保持兩三個月而不褐化；胚乳的誘導時間也較長，需要30d左右。Numerous microbial cultures convert heptachlor to its expoxide.
許多微生物培養基可以把七氯轉化為它的環氧化物。Furthermore the expressions of all the genes in a representative sample were examed by the recently developed method of hybridization to cdna arrays. this was intended to strengthen the theoretical background for the screening of norway spruce genotypes with low lignin content. the calli of the transformed sublines a78 - 3, a78 - 4, a78 - 5 and the untransformed control a95 : 88 : 22 were successfully induced to form mature embryos from which plantlets were established
以轉基因亞系a78 - 3 、 a78 - 4 、 a78 - 5和未轉基因對照a95 : 88 : 22的細胞愈傷組織為實驗材料，以dkm和lp - m熟化培養基培養五周后，再以1 4sh萌發培養基培養四周，成功地誘導形成了胚胎，並再生成新的小植株，萌發成活率達到80 。Soya peptone is an enzyme digest of soya, this product contains carbohydrate, widely used in culture media and often used for the cultvation of many fastidious organisms and where rapid, luxuriant growth is required
性狀：本品系由大豆為原料，經酶消化而成，富含碳水化合物，廣泛應用於微生物培養基的配製並常用於營養要求高的微生物培養及滿足微生物快速大量生長的需要。Agar powder is a phycolloid extracted from a group of red marine algae, it solube in hot water but insoluble in cold water. as a solidifying agent, supension agent and desissant, it can be used in preparing microbiological culture media, bio - engineering, and food manufacture
性狀：本品有海洋中生長的紅藻為原料精製而成的水膠體，易溶於沸水，不溶於冷水，作為凝固劑、增稠劑、乳化劑、懸浮劑和乾燥劑可應用於微生物培養基、生物工程、食品加工等。Their morphological and physiological characteristics were observed through the strains colony morphology, size, color, growth rate, texture, and spores
用察氏平板培養基分離菌株，根據菌株的菌落形態、大小、顏色、生長速率、質地、生長培養基顏色變化以及菌絲體和抱子的形態特徵進行鑒定。6. transformation system of mustard a serials of kanamycin concentration was added to optimum medium to test the explants resistance capacity of two kinds of mustard. the transformation procedures described were derived from numerous regeneration and trasformation designed to test factors that might affect shoot regeneration, which including length of co - cultivation. those producing the best result parameters were described as below : after the mustard explants were precultured on regeneration medium for 2 days. they were inoculated with agrobacterium for 20 minutes. inoculated explants were co - cultivated for 4 days and in shadow at first 2 days. then transferred to the same medium plus 30 mg / l kanamycin and 500mg / l garb. all of them were transferred to fresh medium every 2 weeks. the kan - resistant plants were regenerated
芥菜外植體高頻遺傳轉化體系的建立在最適培養基上試驗了兩類芥菜的三種外植體對卡那黴素的敏感性、預培養天數、浸菌時間等因素的影響，建立了芥菜高頻轉基因再生體系：取生長4天的芥菜子葉、下胚軸和25天的葉片在分化培養基上（ ms + ba3 . 0mg / l + naa0 . 1mg / l ）預培養2 - 3天後，投入農桿菌菌液中浸染20分鐘，在分化培養基上暗培養2天，正常條件下培養2天後，轉入抗性培養基（ ms ba3The influence of three optimized medium on the growth quality of rooting pear plantlet
三種優化培養基對香梨生根試管苗生長質量的影響3. potato stems and agrobacterium fumefaciens containing recombinant vector were co - cultured at 28cfor 48 hours and transplanted onto callus - inducing medium at 24c for 7 days. and then, the explants were transplanted onto differentiation medium and cultured at 24c for 21 days. resistant buds rooted and grew into plants in medium with kanamycin for 20 days, and 83 plants were obtained
3將含外源基因的根癌農桿菌與馬鈴薯莖段共培養后在愈傷誘導培養基上培養7天，轉接到分化培養基上分化出抗性芽，抗性芽在生根培養基上生根長成完整植株，共獲83株。Bap performed more important function than kt in differentiation of tall fescue embryogenic calli, but better results could be achieved with combination of 2mg / l bap and 0. 5mg / l kt. at this cytokinin level, 0. 5mg / l naa was recommended to obtain the highest callus regeneration frequency. plant regeneration could be evidently boosted when embryogenic calli were pre - differentiated on high - osmoticum medium with 60g / l sucrose, and / or when the pre - differentiated compact calli were differentiated on differentiation medium solidified with l0g / l agar
高羊茅胚性愈傷組織分化時， bap的作用要比kt大，但2mg lbap與0 . 5mg lkt配合可獲得更佳的效果；在該細胞分裂素水平下，生長素naa用0 . 5mg l ，愈傷組織再生率最高；胚性愈傷組織先在含60g l蔗糖的分化培養基上高滲預分化，以及經高滲預分化后的緻密愈傷組織在瓊脂濃度為10g l的分化培養基上分化，能明顯促進愈傷組織的植株再生；在分化培養基中添加脯氨酸導致愈傷組織再生率下降，但同時有減少白化苗再生的趨勢。The author discussed the determination of selection pressure, measured the growth of calli and analyzed the influences of regeneration media on the regeneration of resistant calli in order to get some parameters to enhance the indica rice transformation frequency of particle bombardment
從秈稻基因槍轉化過程中選擇壓的確定、愈傷組織的生長規律以及不同分化培養基對抗性愈傷出苗率的影響上，探討了可能提高秈稻基因槍轉化頻率的參數。Uniform design was employed to optimize the culture conditions and the component of culture medium including carbon source, nitrogen source, phosphor source, growth factors, inorganic salts and precusor as well
利用均勻設計原理進行實驗設計，以優化培養基中碳源、氮源、磷源、生長因子、前體物及無機鹽成份的配方以及細菌培養條件。分享友人