生物病毒分表 的英文怎麼說

中文拼音 [shēngwùbìngdúfēnbiǎo]
生物病毒分表 英文
virus classification
  • 生 : Ⅰ動詞1 （生育; 生殖） give birth to; bear 2 （出生） be born 3 （生長） grow 4 （生存; 活） live;...
  • 物 : 名詞1 （東西） thing; matter; object 2 （指自己以外的人或與己相對的環境） other people; the outsi...
  • 病 : Ⅰ名詞1 （疾病; 失去健康的狀態） illness; sickness; disease; malum; nosema; malady; morbus; vitium...
  • 毒 : Ⅰ名詞1 （對生物體有害的性質或物質; 毒物） poison; toxin 2 （毒品） drug; narcotics 3 （姓氏） a s...
  • 分 : 分Ⅰ名詞1. （成分） component 2. （職責和權利的限度） what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] （料想） judge
  • 表 : Ⅰ名詞1 （外面;外表） outside; surface; external 2 （中表親戚） the relationship between the child...
  • 生物 : living things; living beings; organisms; bios （pl bioi bioses）; biont; thing; life生物材料 biol...
  • 病毒 : [醫學] virus; inframicrobe （濾過性）
  1. In trpsin tolerance assay. this virus could resist to 1 % trpsis at 37 in an hour. in acid tolerance assay, this virus was resistant to ph3. 0 and ph5. 0 at 37 in 2 hours, and the average infection litre of the virus decreased little. in heat assay, at 50, the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree. among these conditions, when at 50 in 30 minutes. the average infection litre of this virus decreased over 2 tilre. and when al 50 in an hour, cpe of ihis virus disappeared. when time was set for an hour. but with processed in different temperature as 50 60 70, 80, the virus losl the multiplication capacity complelely. in biological assay, we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory. then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second. with f81 cell line, after passaged for 12 times continuously with low concentration of fcs. the virus could produce cpe. however, with vero cell line. the virus could not procuce any cpe after many passages. the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig, fowl and cavy. by neutrolizaion assay, dxmv could be identified as a kind of ccv

    理化學研究表明，該病毒為rna病毒，對氯仿、乙醚敏感；胰酶試驗中，經37 、 1小時處理的病毒，仍然能夠在貓源細胞fcwf細胞上生長，並且毒力基本保持不變；耐酸性試驗中，病毒分別在ph5 . 0和ph3 . 0經37作用2小時，毒力僅下降一個滴度；耐熱性試驗中，該病毒在恆定溫度50 ，設定不同時間，從5分鐘到150分鐘，毒力均有不同程度下降，其中， 50作用30分鐘，病毒平均滴度下降2個單位； 50 ， 60分鐘， cpe消失；恆定時間1小時，設定不同溫度（ 50 - 60 - 70 - 80 ） ，病毒在細胞上完全喪失增殖能力， cpe消失。生物學試驗，利用實驗室現有條件，選擇不同的細胞系對該病毒進行培養，發現該病毒對貓源細胞fcwf最敏感； mdck細胞次之； f81細胞經多次傳代，亦可出現cpe ；而vero細胞則不敏感。血凝試驗表明，該病毒對豬、雞、人及豚鼠的紅細胞均無血凝性。
  2. Phytoalexins are low molecular weight chemicals that plants produce and accumulate in response to infection especially of fungal origin. sakuranetin is a kind of flavanone phytoalexin isolated from ultraviolet - irradiated rice leaves. recent research work on flavanone phytoalexins represented by sakuranetin is reviewed. interesting novel structures, stucture - activity relationships and synthetic methods are discussed

    植物抗毒素是植物受到外界病原微生物侵擾后所產生並積累的一類具有抗菌活性的小分子物質，櫻花素是從水稻稻瘟病感染組織中分離鑒定的一種黃烷酮類植物抗毒素.對以櫻花素為代表的水稻抗毒素及其類似物的結構與活性、黃烷酮類植物抗毒素合成方法的研究概況進行了綜述
  3. The sucking mouse brain were inoculated with mdj - 01 strain to make electron microscopic examination, results showed that the virus was a spheral particle with membran which had a diameter of about 40 nm. by indirect fluorescent antibody test mdj - 01 strain was identified with tbev. a part of region encoding e protein was expanded by rt - pcr and sequenced. the nucleotide sequences of two strain viruses were compared with sequences in genbankjsequence homology analyses revealed mdj - 01 strain and senzhang strain had the highest homology with tbev oshima5 - 10, respectively, which were 95 %, 94 %. mdj - 01 strain was identified with tbev again

    應用間接免疫熒光試驗進行血清學鑒定，結果表明mdj - 01株為tbev 。通過rt - pcr技術擴增部分e蛋白序列並測序，在genbank上進行同源性比較，發現mdj - 01株和森張株與tbevoshima5 - 10株的同源性最高，分別為94 、 95 ，從分子生物學水平上進一步證明mdj - 01株病毒為tbev 。在鑒定的基礎上，本實驗對兩株病毒進行了核苷酸全序列測定。
  4. The reasults are summed up as following : 1 the study on chromosomes and mitoses of bmn cells the cell line, bmn, is a silkworm cell line widely used in silkworm molecular genetics, cell engineering, gene engineering and baculovirus expression system but whose genetics and cytobiology studies are nearly untouched. the chromosomes and mitoses of the bmn cells are researched by the air - drying method and culturing cells on cover glasses

    同時，還通過原代培養實驗對新的家蠶胚胎細胞系的建立進行了探索和嘗試，並對家蠶胚胎原代培養過程中出現的細胞和組織類型進行了觀察、探討與研究。 1bmn細胞有絲分裂及染色體研究bmn細胞是家蠶分子遺傳學，細胞工程、基因工程和桿狀病毒表達系統中廣泛應用的家蠶細胞，但其遺傳學和細胞生物學背景知之甚少。
  5. Recovery estimated from the safh4 plant line indicates that 9 ( jl g of pure active scfv can be obtained per gram of fresh leaf material, on a laboratory scale. the production of the scfv antibody proteins in plant root exudates was also addressed. the scfv antibody protein was continuously secreted from the transgenic tobacco roots into a simple hydroponic medium at 630 to 760 ng g - 1 dry weight of root day - 1

    在水培條件下，轉基因煙草根可連續分泌具有活性的重組抗乙肝病毒表面抗原pres1 （ 20 - 47 ）單鏈抗體進入到液體培養液中，不須破壞植物即可連續獲得重組單鏈抗體，為利用植物生物反應器連續生產單鏈抗體開辟了新途徑。
  6. Addressing a press conference in murray building he said it was " very heartening " that cuhk microbiologists working with prince of wales hospital staff had identified it as a member of the paramyxoviradae family of viruses that includes measles and mumps

    楊醫生今日在美利大廈舉行的新聞簡報會上表示，對于香港中文大學微生物學家與威爾斯親王醫院人員已確定該病毒，令人十分鼓舞。該病毒屬于副黏液病毒科的病毒家族，病毒成員包括麻疹病毒及腮腺炎病毒。
  7. In order to develop recombinant poifn - a preparations preventing porcine viral infectious disease, and to further expound the research of cytokines in the field of molecular biology, mature poifn - a was cloned and expressed in e. coli expression system in the study

    為了在我國研製生物工程重組豬干擾素類制劑，防制豬病毒性傳染病和進一步開展豬干擾素分子生物學研究，本文進行了豬干擾素基因（ poifn ）的克隆、表達及其重組蛋白抗豬瘟病毒的研究。
  8. This dissertaion, on the basis of the other studies, on the one hand, researched and analysed the chromosomes and mitoses of the cell line - bmn, which is widely used in the silkworm baculovirus expression system as an engineering cell. on the other hand, the dissertation attempted to explore establishment of the new silkworm embryoni cell line by primary culture

    同時，家蠶體外細胞培養研究的進行，不僅可以為家蠶細胞生物學的基礎理論研究提供良好的研究系統，而且在家蠶資源的開發與利用方面也具有重大的意義。本研究在借鑒前人研究的基礎上，一方面對現在廣泛應用於家蠶桿狀病毒表達系統的工程細胞? bmn細胞的染色體和有絲分裂進行了研究與分析。
  9. The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action, thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained. in this study, we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template. the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons

    本研究設計以c tamrna為模板的反義cdna片段，從c ta基因5 』端第94位到500位核苷酸段設計引物，目的片段407bp ，覆蓋第116和188位兩個aug密碼子，也包含了第一外顯子和第二外顯子間的剪接位點：用常規分子生物學方法構建了反義片段的腺病毒表達載體（ padeasy - 1系統） ；腺病毒載體經hek293細胞包裝產生含反義片段的重組腺病毒，用氯化銫密度梯度離心法獲得純化的高滴度腺病毒；進行體外基因轉移，分別用反義片段真核表達載體轉染p388d1細胞和用重組腺病毒感染hela細胞，觀察導入的c ta基因反義rna抑制細胞內組成型或誘導型c ta基因表達的作用，從而達到調控mhc -類分子表達的目的。
  10. A chp spokesman said pivs are spread by respiratory secretions through close contact with infected persons or contact with contaminated surfaces or objects

    生防護中心發言人表示，副流感病毒是透過接觸受感染人士的呼吸道分泌物，或接觸受污染的物件表面及物件而傳播。
  11. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
  12. A small number of flu viruses resistant to tamiflu, a top antiviral drug, have been detected in europe, health authorities said

    歐洲已經偵測到少部分能抵抗強效抗病毒藥物克流感的感冒病毒，衛生主管單位表示。
  13. To overcome these limitations, we produced recombinant an - giostatin in a baculovirus expression system. here we report the expression and analysis of recombinant human angiostatin produced by insect cells infected with recombinant angiostatin - baculovirus. methods generation of recombinant baculovires by co - transfection

    為克服這些困難，獲得具有高活性的angiostatin表達，為進一步研究及應用奠定物質基礎，我們通過桿狀病毒表達系統表達重組的angiostatin ，本研究就是在昆蟲細胞中表達和分析angiostatin ，並研究其生物活性。
  14. Ingl plasndd was a1so prepared and delivered to the npc cell. subsequenly, v mbl was also de1ivered to the cell and intensity fluorescence was observed by nuorescence confocal ndcroscope. the degree of fluorscence enhancemen can be wttatively obtaind through anscence spectr8

    4這種探針對乙肝病毒陽性和陰性病人血清的檢測有顯著性差異，生物素化的分子信標可以用橋式結構固定在玻璃片表面，製成dna晶元，根據晶元的熒光可以快速檢測到靶g dna的存在。
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