生物素標記的 的英文怎麼說
中文拼音 [shēngwùsùbiāojìde]
生物素標記的
英文
biotin labeled- 生 : Ⅰ動詞1 (生育; 生殖) give birth to; bear 2 (出生) be born 3 (生長) grow 4 (生存; 活) live;...
- 物 : 名詞1 (東西) thing; matter; object 2 (指自己以外的人或與己相對的環境) other people; the outsi...
- 素 : Ⅰ形容詞1 (本色; 白色) white 2 (顏色單純) plain; simple; quiet 3 (本來的; 原有的) native Ⅱ名...
- 標 : Ⅰ名詞1 [書面語] (樹梢) treetop; the tip of a tree2 (枝節或表面) symptom; outside appearance; ...
- 記 : Ⅰ動詞1 (把印象保持在腦子里) remember; bear in mind; commit to memory 2 (記錄; 記載;登記) writ...
- 的 : 4次方是 The fourth power of 2 is direction
- 生物 : living things; living beings; organisms; bios (pl bioi bioses); biont; thing; life生物材料 biol...
- 標記 : (標志; 記號; 貨物標記) tab; sign; stamp; peg; label; mark; flag; blip; notation; fleck; track; ...
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Our previous studies showed existence of apoplast cam in the plant cell and cam had many extracellular functions. so it supposed cam may be one of important extracellular polypeptides and trigger the intracellular signal transduction by binding the receptor. in this study, radiolabelled ligand is used to investigate the binding characteristic of cam and a. thaliana protoplasts. and chemical crosslinking is employed to explore binding proteins in the membrane. at first, ( 35 ) ~ s - cam was produced using ( 35 ) ~ s - labeled amino acid mixture in e. coli. sds - page and autoradiograph indicated high - purified, high - specific radioactivity ( 35 ) ~ s - cam was obtained. electrophoresis of ( 35 ) ~ s - cam is the same as that of unlabeled cam with ca ~ ( 2 + ) or egta ; a quatitive of protoplasts was prepared by enzymolysis
首先,用~ ( 35 ) s標記的氨基酸混合物喂養工程菌成功地制備了~ ( 35 ) s標記的擬南芥鈣調素( ~ ( 35 ) s - cam ) , ~ ( 35 ) s - cam純度高、放射活度高、 ca ~ ( 2 + )與egta存在時的電泳行為與未標記cam相同,可作為一種高靈敏性的探針用於進行受體學分析實驗;用擬南芥種子誘導愈傷,通過酶解制備了大量原生質體。The capture of the labeled antibody ? analyte complex for signal generation can be achieved with the antibody immobilized in a defined area of nitrocellulose membrane as mentioned
對產生信號的標記抗體抗原復合物的捕獲,是用抗體固定在已經提到的硝酸纖維素的特定區域內來達到的。Although this method gives high sensitivity, the radioactive labels present many problems such as a potential hazard to analyst and environment, which limited its application in dna diagnostic laboratories. in order to overcome these problems a serious of non - radioactive dna probes such as fluorescent, chemiluminescent and electrochemical probes have been developed. although these new methods display many advantages, they have not been used to take place completely the traditional method because of low sensitivity or complex equipment or other shortcomings
自20世紀80年代以來,各種非同位素如酶、熒光素、生物素、地高辛標記的化學發光法和熒光分析法以及以電活性物質做標記的電化學方法相繼問世,這些方法雖然在一定程度上克服了同位素標記的缺陷,但由於存在靈敏度不夠高或檢測系統龐雜或儀器價格昂貴或標記物不穩定等缺陷,還不能完全取代傳統方法。The dendrimers would carry one of the stable isotopic or fluorescent labels to identify the presence or absence of a protein that can be further deeloped for use as a disease indicator, or biomarker
攜帶有穩態同位素或熒光標記的樹狀高分子可以鑒別標本中是否存在某種蛋白質,從而可以將其作為某種疾病的化學指標或生物標記。But these techniques can not provide physiological ph and ionic strength, and radio - iodination of heparin required for ace endangers health of human and environment. for avoiding the radiological hazards and reflecting directly the binding characteristic of heparin to protein
但親和層析柱易被污染失活, ace技術不能提供生理ph范圍和離子強度及ace技術必需的標記肝素的放射性物質對人和環境具有很大危害。They were capable of detecting 50 pg of purified total rna by a colorimetric assay and 5 pg by a chemiluminescent assay. the nonradioactive probe produced with pcr amplification were particularly suitable for practical diagnosis, as they are sensitive and can be rapidly prepared in large quantities. the two assay methods were 260 times and 26 times as sensitvity as r - page respectively
利用含pstvd單體的pgem - 7z質粒,通過pcr擴增技術,用生物素標記制備cdna探針,進行雜交反應檢測pstvd ,其中通過化學顏色反應進行判讀靈敏度可達到50pg ,而用化學發光反應進行判讀靈敏度可達到5pg ,分別是r - page檢測靈敏度的260倍和26倍。The author reviewed the detection measures of prunus necrotic ringspot virus, and related the research progression of the pathogen detection technology inside and outside. the template amplication technology include pcr assays ^ nasba and so on. the cdna and crna probe which labeled with the isotope % biotin or dig, the offset probe and the peptide probe can be applied to magnify the signal. pcr - gene scan assays and pcr agilent chip chamber combine the template amplication and the signal magnification
本文回顧了李壞死環斑病毒的檢測方法,較全面地評述了國內外病原物檢測技術研究進展:在模板的擴增有各種pcr技術、 nasba技術;在信號的放大有同位素、生物素或dig標記的cdna和crna探針,分支探針和肽核酸探針;模板擴增和信號放大相結合的有pcr -基因掃描技術、 pcr安捷倫晶元實驗室技術;模板擴增和雜交以及信號放大相結合的有pcr - elisa技術、實時熒光pcr技術、生物晶元技術。In the may issue of cancer epidemiology, biomarkers & prevention, researchers based at the national cancer institute and fred hutchinson cancer research center report that lycopene, an antioxidant predominately found in tomatoes, does not effectively prevent prostate cancer
來自美國國家癌癥研究所和佛瑞德?哈金森癌癥研究中心的研究人員在《癌癥流行病學、生物標記物和預防》 5月刊發表的報告指出,西紅柿中的主要抗氧化成分? ?番茄紅素,不能有效地預防前列腺癌。The method was making bone lacuna artificially in rabbits " condyles, and implanting home - made porous p - tcp. then a serial of rabbits were killed every month and condyles, including material, were taken out. by force of the histological analysis measures such as he, tbs and tdl, an in - depth study went along with p - tcp, the new - born and the interface between the materials and the new - born in the implanted region, we may attempt to ascertain the osteogenetic process of new bone when porous p - tcp bioceramics repairing bone defects
實驗方法是,人為造成兔股骨髁部骨缺損,將自製的多孔- tcp生物陶瓷植入,然後逐月取實驗兔處死、取材,利用he染色、甲苯胺藍染色、四環素雙標記法等組織學分析手段,對材料植入區的- tcp 、新骨及材料與新骨的界面進行研究,探討多孔- tcp生物陶瓷修復骨缺損時,新生骨的形成過程。We screened the cdna expression library by using 12 b - deoxoartemisinyl - ( 4 ' - oxyacetic acid ) phenyl ether conjugated with bsa. one of the screened positive clones was inserted tctp of plasmodium falciparum from hainan. the tctp was cloned to express protein in order to provide materials for further study
把青篙素類衍生物: 12b一二氫青篙素( 4 』一氧乙酸基)苯醚用bsa標記作為探針,對惡性瘧原蟲海南株cdna表達文庫篩選,分離出插入了惡性瘧原蟲海南株tctp基因的陽性單克隆。So we observed the distribution and the relative amount of the polysaccharide - protein complex and glycosyls in order to find out the possible functions and changing rules of these substances
方法:採用愛爾藍及生物素標記的植物血凝素方法對兔下頜鈦種植體周圍組織中的多糖-蛋白復合物及糖基進行觀察。Anti - igg antibody was immobilized onto the surface of gold electrode modified with a thin layer of protein a. biotin labeled anti - igg antibody and hrp - streptavidin were premixed to form network complex to be used as probes for amplifying the sensing response of antibody - antigen interaction
抗體通過單層蛋白a固定到金電極表面,預先處理好的生物素化抗體和辣根過氧化物酶( hrp )標記的親和素形成的網狀復合物作為免疫反應信號的放大探針。Standard test method for determining the biodegradability of radiolabeled polymeric plastic materials in seawater
海水中放射性同位素標記的聚脂塑料的生物降解性的測定的標準試驗方法Wide variety of modifications from biotinylation to phosphorylation, to dye labeling, and much more
多種修飾接入生物素磷酸化染色標記等等藥用的At 6 months after ahst treatment, the level of c - peptide, a marker that shows the presence of the body ' s own produced insulin, was significantly greater than the pre - treatment values, and did not change at 12 and 24 months
幹細胞移植后6個月, c -肽水平,反映體內自身產生胰島素的標記物,比治療前顯著升高,在12個月和24個月時也沒有改變。Conclusion : pmp technology can be useful in identifying potential biomarkers in patients with copd. panels of * ed serum markers are associated with important clinical predictors of outcome in these patients
結論: pmp技術有助於鑒別慢阻肺患者潛在的(血清)生物標志物。選定的血清標記群與患者重要的臨床預后因素有相關性。分享友人