生體組織檢鏡 的英文怎麼說
中文拼音 [shēngtǐzǔzhījiǎnjìng]
生體組織檢鏡
英文
vital microscopy- 生 : Ⅰ動詞1 (生育; 生殖) give birth to; bear 2 (出生) be born 3 (生長) grow 4 (生存; 活) live;...
- 體 : 體構詞成分。
- 組 : Ⅰ名詞1 (由不多的人員組成的單位) group 2 (姓氏) a surname Ⅱ動詞(組織) organize; form Ⅲ量詞(...
- 織 : 動詞(編織) knit; weave
- 檢 : Ⅰ動詞1 (查) check up; inspect; examine 2 (約束; 檢點) restrain oneself; be careful in one s c...
- 鏡 : Ⅰ名詞1 (鏡子) looking glass; mirror 2 (幫助視力或做光學實驗的器具) lens; glass 3 (姓氏) a s...
- 組織 : 1 (組織系統) organization; organized system 2 (組成) organize; form 3 [紡織] weave 4 [醫學] [...
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Methods the 54th generation of transformed human embryonic tendon cells and artificial composite materials of carbon fibers ( cf ) and polyglycolic ( pga ) were co - cultured in vitro to construct tet. lt was frozen in liquid nitrogen with four kinds of cpa for 2 months. post - thawed quickly and transplanted into hind limbs of nude mice, and repaired the defects of achilles tendon. after 2, 4, 6, 8, 12 weeks, the morphological, histological, ultrastructure, short tandem repeat loci and immunohistochemistry examination were detected, and biomechanical strength of tet were examined. result tendon cell survived and could secret type i collagen after 12 weeks to transplanted into nude mice. in the group of dmso + raffmose + kh2o4, vacuole in mitochondrion degraded i tendon cell ranged in order, abundant collagen fibers were found and linked each other and the biomechanical strength was increased as time elapsed. c onclusion dmso + raffmose + kh2o4 could protect tet in deep low temperature
組織工程肌腱制備完成後在四種抗凍劑保護下液氮凍存2月;快速復溫后植入裸鼠以修復跟腱缺損, 2 、 4 、 6 、 8 、 12周后取出,觀察形態學、組織學、電鏡和免疫組織化學變化,短串聯重復位點檢測和生物力學變化。結果實驗組組織工程肌腱體內植入12周后仍有肌腱細胞存活並分泌型膠原;隨著時間延長, 10二甲基亞碸( dmso ) +棉子糖( 30mmol l ) + kh _ 2po _ 4 ( 25mmol l )組線粒體空泡減少,肌腱細胞排列整齊,膠原纖維增粗並連接,抗拉強度增高。Methods : in cultured lung explants without serum, the lipid component synthesis of pulmonary surfactant was evaluated in [ 3h ] - choline incorporation ; mrna content of phosphocholine cytidylyltransferase ( cct ) in lung explants was investigated in rt - pcr ; the changes of the ultrastructure of the at ii cells were observed with electron microscope ; the expression of nmdar1 subtype was observed in immunohistochemistry staining ; nitric oxide synthase ( nos ) activity, nitric oxide ( no ) content, superoxide dismutase ( sod ) level, malondialdehyde ( mda ) content and lactae dehydroase ( ldh ) level were determined by biochemistry methods. results : 1. influence of glutamate on synthesis of the lipid component of pulmonary surfactant ? with l - arginine, glu inhibited [ 3h ] - choline incorporation with good dose - dependence and time - dependence ; ( 2 ) mrna content of cct of the glu treatment groups was decreased ; ( 3 ) glu increases the release of ldh in cultured lung explants ; ( dwith electron microscope histochemistry, glu induced the changes of the ultrastruture of at ii iv cells
方法:採用成年大鼠肺組織無血清培養,運用[ ~ 3h ] -膽堿摻入法測定ps主要脂質磷脂酰膽堿( pc )合成量; rt - pcr擴增檢測肺組織中pc合成限速酶磷酸膽堿二胞苷酰基轉移酶( cct ) mrna含量;透射電子顯微鏡法觀察肺泡型上皮細胞和ps系統超微結構的變化;免疫組織化學染色檢測glu的受體nmdar1亞單位的表達;生化測定肺組織乳酸脫氫酶( ldh )釋放量和肺組織勻漿中一氧化氮合酶( nos )活性、一氧化氮( no )生成量、超氧化物歧化酶( sod )水平以及丙二醛( mda )含量。
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