甲基化清蛋白 的英文怎麼說

中文拼音 [jiǎjīhuàqīngdànbái]
甲基化清蛋白 英文
methylated albumin
  • 甲 : Ⅰ名詞1 （天乾的第一位） the first of the ten heavenly stems2 （爬行動物和節肢動物身上的硬殼） she...
  • 清 : Ⅰ形容詞1 （純凈） unmixed; clear 2 （寂靜） quiet 3 （清楚） distinct; clarified 4 （一點不留） w...
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 甲基 : methyl; methylium; carbinyl; hexanone
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  1. Aminated and hydroxylated polysulfone membranes were prepared by amination and hydroxylation reaction, respectively. then bovine albumin ( bsa ) - fixed membranes were obtained by crossed - linking albumin into porous membrane with 1, 1 ' - carbonyldiimidazole and bisoxirane reagents, respectively. a mathematical model for facilitated transport in asymmetric membranes with fixed site carriers was derived by assuming an instantaneous, microscopic concentraion fluctuation in the membrane

    以氯甲基聚碸為基材，通過相轉化法制備出具有底部貫通孔的非對稱膜，通過胺化和羥基化反應，分別制得胺化聚碸膜和羥基化聚碸膜，再採用羰基二咪唑和雙環氧烷兩種活化試劑對其進行活化，將牛血清白蛋白固載在膜內，獲得固定白蛋白促進傳遞膜。
  2. The results of sds - page and western blot showed that the culture supernatant of transforments contained the correct glycosylated e2 protein and the recombinants could still secretedly express the specific proteins after culturing for 8 generations

    經甲醇誘導表達后， sds - page和westernblot結果證明了p . pastoris培養上清液中含有正確糖基化的e2蛋白，表達量約250mg / l 。
  3. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
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