留粒篩 的英文怎麼說
中文拼音 [liúlìshāi]
留粒篩
英文
retaining screen-
However, as my first crop was but small i had no great difficulty to cut it down ; in short, i reap d it my way, for i cut nothing off but the ears, and carry d it away in a great basket which i had made, and so rubb d it out with my hands ; and at the end of all my harvesting, i found that out of my half peck of seed, i had near two bushels of rice, and above two bushels and half of barley, that is to say, by my guess, for i had no measure at that time
因為我既不知道怎樣把穀粒磨成粉，甚至根本不知道怎樣脫谷，怎樣篩去秕糠即使能把穀粒磨成粉，我也不知道怎樣把粉做成麵包即使做成了麵包，也不知怎樣烤麵包。另外，我想多積一點糧食，以保證不斷供應。為此，我決定不吃這次收獲的穀物，而是全部留起來做種子，待下一季再播種。The mutant pel - d92l was expressed in pichia pastoris gs115, sds - page detection showed that the expression product pel - d92l - gs is different from pel - gs, and its " yield decreased dramatically, the themostability of pel - d92l - gs is also different from the pel - gs, but their optimum temperatures are same. 3. directed evolution of pel through random mutagenesis mutagenesis pcr carried out in error - prone conditions was used on the vector psk - pel, using the oligos " beginning " and " end ", homologous to the 5 ' and to the 3 " ends of the gene of pel respectively
三、 pel基因的隨機誘變用易錯pcr方法對pel基因進行隨機誘變， pcr產物與ppic3 . 5k連接，轉化大腸桿菌，獲得的混合質粒電轉化畢赤酵母gs115 ， omm平板篩選適于低溫或對熱穩定的重組子，篩選獲得一株最活反應溫度、熱穩定性、發酵酶活均有提高的突變體pel - ep5 - gs ，其最活反應溫度為45 ，比野生型高出5 ； 40處理30min殘留活性為56 ，大大高於野生型的6 ；初始ph7 . 3528條件下培養72h ，發酵上清酶活為325u / ml 。
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