畢赤氏 的英文怎麼說
中文拼音 [bìchìshì]
畢赤氏
英文
pichia-
Methods : an artificial gene for fl extracellular domain cdna was synthesized by using favored genetic codons of pichia pastroris. by inserting human fl extracellular domain cdna coding 156 amino acid resi dues into pichia pastoris expression vector ppic9k containing aox1 promoter and the sequences of alpha secreting signal peptides, a recombinant expression plasmid ppic9k - fl was constructed, and integrated into the alcohol oxidase region of the host genome
為了提高外源基因的表達量，我們根據畢赤氏酵母偏愛密碼子人工合成了編碼fl胞外區156個氨基酸的cdna序列，目的序列被定向克隆到酵母分泌型表達載體ppic9k質粒上，構建ppic9k - fl表達質粒。Proteins of expression in multi - copy recombinant were able to react with monkey - anti - pzp3 a antibody and rabbit - anti - hcg antibody specifically. cone i us i on : recombinant pzp3 a hcg p - ctp109 - 145 was constructed and expressed in the yeast pichia pastoris successfully. it provides a foundation for further contraception vaccine research
結論：重組質粒ppic9k - pzp3 - hcg - ctp構建成功，並在巴氏畢赤酵母中獲得重組抗原pzp3 - hcg - ctp的表達，為探討重組多特異性避孕疫苗抗生育效能的研究建立基礎。After linerization, the recombinant plasmid was transformed into pic hia pastoris by electroporation, which then were cultured in md plate free of histidine, from which the positive colones were propagated
重組質粒線性化后，用電擊法將重組質粒轉化入巴氏畢赤酵母，在缺組氨酸的md板上篩選陽性菌落，然後用不同濃度的g418 ? ypd板篩選多拷貝插入單菌落。Biological assay proved that the expressed product could stimulate the proliferation of cd34 + hematopoietic cells. conclusion : flt - 3 ligand extracellular domain was successfully expressed
體外活性實驗表明，畢赤氏酵母表達的重組fl蛋白可以有效地刺激造血幹細胞cd34 「的增殖。Multicopy integrants were screened with g418 from pichia pastoris which contains recombinant plasmid, and induced with methanol to secrete interesting peptide. the supernate of pichia pastoris culture was analysed by sds - page and western blotting. a reactive band, which the apparent molecular weight is 36kd, can be detected with sheep anti - hcmv polyclonal antibodies
重組質粒轉化巴氏畢赤酵母， g418篩選出多拷貝插入的單克隆，甲醇誘導多拷貝插入的單克隆酵母細胞分泌目的蛋白，培養液上清經sds - page電泳分析，在蛋白質印跡中檢測到培養液上清有一表觀分子量為36kd ，能與羊抗hcmv多克隆抗體發生發應的條帶。High level expression of human recombinant albumin gene in the yeast pichia pastoris
人血管內皮生長因子受體2胞外段在畢赤氏巴斯德酵母中的表達Sds - page showed efficient expression and secretion of the rhfl. the highest yield ( 108 mg / l ) was obtained when expression was induced with 0. 5 % methanol for 96 hours
Western雜交實驗出現了可被抗體特異性識別的雜交帶，證實此條帶即為重組畢赤氏酵母km7lppicgkil分泌表達的重組fl蛋白。The study lays a foundation for further application of the expressed product in expansion of hematopoietic stem cells and tumor immunotherapy. last, recobinant human fl can be used to generate large numbers of antigen - presenting dendritic cells from cd34 + peripheral blood progenitor cells that might be ideally used for immunotherapeutic approaches
綜上所述，本實驗根據畢赤氏酵母偏愛密碼子構建了高效分泌表達fl的工程菌株，其分泌表達的重組fl蛋白具有與天然蛋白相似的生物學功能，為獲取大量重組fl蛋白，進一步研究fl的生物功能奠定了工作基礎。分享友人