畢赤酵母 的英文怎麼說

中文拼音 [bìchìjiàomǔ]
畢赤酵母 英文
pichia pastoris
  • 畢 : Ⅰ動詞（結束 ; 完成） finish; complete; terminate; conclude Ⅱ副詞[書面語] （全; 完全） all; altoge...
  • 赤 : Ⅰ形容詞1. （紅色） red 2. （忠誠） loyal; sincere; single-hearted 3. （光著; 裸露） bare Ⅱ名詞（姓氏） a surname
  • 酵 : 動詞（發酵） ferment; leaven
  • 母 : Ⅰ名詞1 （母親） mother 2 （泛指女性長輩） one s elderly female relatives 3 （配套的兩件東西里的凹...
  • 酵母 : yeast酵母浸液[提取液] yeast extract; 酵母聚糖 zymosan; 酵母片 aluzyme; 酵母細胞[植物] yeast plant; yeast cell
  1. Thrombin inhibitor ; expression in methylotrophic yeast pichia pastoris ; anticoagulant

    山蛭素凝血酶抑制劑畢赤酵母表達抗凝劑
  2. Expression of bovine interleukin - 2 gene in pichia pastoris

    2在畢赤酵母中的表達
  3. Expression of mn superoxide dismutase gene from bacillus cereus m22 in pichia pastoris

    葡萄糖苷酶基因在畢赤酵母中的表達研究
  4. Secreted expression of mannanase gene in pichia pastoris and anylysis of enzymic properties

    植酸酶基因在畢赤酵母中表達及表達酶性質研究
  5. Secreted expression of trichoderma reesei endo - - glucanase gene in pichia pastoris and anylysis of enzymic properties

    22錳超氧化物歧化酶基因在畢赤酵母中的表達
  6. Identification of expressed human chorionic gonadotropin subunit in pichia pastoris

    人絨毛膜促性腺激素在畢赤酵母中的表達及鑒定
  7. Yeast gs115, one kind of pichia pastoris, has been used to express varied heterologous proteins

    Gslls是畢赤酵母uichiapastoris ）的一種菌株，己被用於多種外源蛋白的表達。
  8. Cloning and expression of human lysozyme gene in pichia pastoris

    人溶菌酶基因的克隆及其在畢赤酵母中的表達
  9. Cloning and expression of a musca domestica defensin gene in pichia pastoris

    的克隆及在畢赤酵母中的表達
  10. Heteroexpression of rhizopus arrhizus 6 - fatty acid desaturase gene in pichia pastoris

    脂肪酸脫氫酶基因在畢赤酵母中的表達
  11. Overexpression of selenomonas ruminantium phytase with high specific activity in pichia pastoris

    的高比活植酸酶基因在畢赤酵母中的高效表達
  12. Cloning and expressing of cellulase gene from thermophilic fungi chaetomium thermophilum ct

    的克隆及在畢赤酵母中的表達
  13. The deleted mutant pap gene was also cloned into yeast secreted expression ppic9k vector to form ppic9k ~ 3, then the vector was transferred into pachia pastoris gs115 strain. the specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50 - 60 u g per millilitre measured by uv - absorbed methods in the supernatant of the medium via high density fermentation. sds - page results showed that there was one protein band in the gel which molecular weight was about 34ku

    將缺失型pap基因克隆于酵母分泌型表達載體ppicgk構成重組載體，然後導入畢赤酵母（ p8chianastoris ）菌株gslls細胞中，在甲醇的誘導下，經過酵母高密度發酵進行pap的表達，經sds page分析，結果表明，在培養基上清液中含有一明顯的特異性蛋臼條帶，大小為34ku ，經western blotting分析，該蛋白與法國pap抗血清有特異性反應，體外活性檢測表明該蛋白對tmv的侵染性具有高度的抑制性，說明該pap基因在畢赤酵母gs中也得到了正確表達。
  14. Effective expression of chaetomium thermophilum ct2 cellobiohydrolase in pichiapastoris

    在畢赤酵母中的高效表達
  15. Proteins of expression in multi - copy recombinant were able to react with monkey - anti - pzp3 a antibody and rabbit - anti - hcg antibody specifically. cone i us i on : recombinant pzp3 a hcg p - ctp109 - 145 was constructed and expressed in the yeast pichia pastoris successfully. it provides a foundation for further contraception vaccine research

    結論：重組質粒ppic9k - pzp3 - hcg - ctp構建成功，並在巴氏畢赤酵母中獲得重組抗原pzp3 - hcg - ctp的表達，為探討重組多特異性避孕疫苗抗生育效能的研究建立基礎。
  16. The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides

    進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。
  17. After linerization, the recombinant plasmid was transformed into pic hia pastoris by electroporation, which then were cultured in md plate free of histidine, from which the positive colones were propagated

    重組質粒線性化后，用電擊法將重組質粒轉化入巴氏畢赤酵母，在缺組氨酸的md板上篩選陽性菌落，然後用不同濃度的g418 ? ypd板篩選多拷貝插入單菌落。
  18. Expression of endo - - mannanase gene from trichoderma reesei in pichia pastoris

    甘露聚糖酶基因在畢赤酵母中的表達
  19. The expression of oligo - 1, 6 - glucosidase from bacillus subtilis in pichia pastoris

    基因在畢赤酵母中的表達及酶學性質研究
  20. Transforming to pichia pastoris gene encoding mussel adhesive protein

    貽貝粘合蛋白基因轉化巴斯德畢赤酵母
分享友人
分享到 Line

分享到臉書