Driven by the cauliflower mosaic virus ( camv ) 35s promoter, the er - shsp over - expressed constitutively. the growth and the phenotype of transgenic plants can be used for researching the function of er - shsp in improving tomato ' s cold resistance and the er - shsp chaperones function in vivo. after degested by kpnl and xbal enzymes from the pbs - er plasmid, the gene er - shsp - lehsp21. 3 was inserted into the prokii vector to construct an eukaryotic expressing vector
利用基因工程方法,將內質網小分子熱激蛋白基因( endoplasmicreticulum - locatedsmallheatshockproteingene , er - shspgene ) - lehsp21 . 3導入到
番茄體內,使之在植物體中
組成性表達( constitutiveexpression )內質網小分子熱激蛋白( er - shsp ) ,觀察轉基因
番茄在低溫條件下的生長和表型反映,研究er - shsp在提高植物耐寒性中的作用,同時為體內研究er - shsp的分子伴侶機制提供依據。
The results of large - scaled culture show that 48g clone crushed by tissue disintegrator in the course of initial period and its inoculating density is 0. 35g / l, a month later, the clone departed directly and its density is 1. 5g / l, the highest density of each jar could be obtained 410g, the growth speed mostly doubled per week
大規模培養結果表明在20l廣口瓶中(有效水體15l ) , 48g克隆在起始培養時經
組織搗碎機粉碎切割,切割時間10s (細胞段長度約200um ) ,並按0 . 35g l接種,一個月後不粉碎直接分瓶,分瓶密度1 . 5g l ,一個月後最大密度410g瓶,生長速度約每周翻一
番。
4. after having established genetic transformation system with tomato cotyledons as explant and determined the transformable of preculture time, incubation time and co - culture time, we set up the system of high frequency transformation of tomato cotyledons. then hbmp - 3m gene was transferred into tomato via agrobacterium - mqdiated transformation, and the resistant plants to hyg were obtained. by pcr analysis on part of the putative transformants, we identified that hbmp - 3m gene had been integrated into the genome of part of tomato plants. 5. transferred hbmp - 3 gene into tobacco via agrobacterium - mediated transformation and obtained the resistant plants to hyg. trans genie tobacco plants were confirmed by instantaneous expression of gus gene in calli detection, growth and bio - morphology analysis, hyg - resistant experiment and pcr analysis
通過pcr檢測證實部分
番茄抗性植株中已導入hbmp - 3m基因;人骨形成蛋白一3成熟膚基因和全長基因分別轉化
番茄和煙草的研究5 .通過農桿菌介導法將hbmp一3全長基因導入煙草,並且獲得了hyg抗性植株,通過gus基因瞬時表達檢測、轉化植株的生長情況及形態學分析、 hyg抗性鑒定及pc尺檢測,證明目的基因己經整合到煙草基因
組中。
So, we can concluded that over - expressed constitutively er - shsp can improved the tomato ' s cold - resistance indeed
由此我們可以確定, er shsp的
組成性表達確實提高了
番茄的耐寒性。
And campaign groups and into the product pages of a mainstream consumer magazine. stiftung warentest, the consumer organization that publishes test, stirred controversy when it decided to include the ethics information not least because, with of 650, 000 a month, the magazine has a real effect on consumer choices
出版「檢測」雜志的消費者
組織stiftung warentest在決定將道德規范信息作為評價標準后,的確在社會上攪起了一
番論戰,但雜志月銷65萬測的規模也的確對消費者產生巨大影響。
The plant hormone ethylene has a profound role in plant growth and development. a number of ethylene signaling elements have been isolated and identified. although nuclear cascades in ethylene signal transduction pathway from ein3 to erf1 to pr proteins has been established in arabidopsis thaliana, a similar route from ein3 to erebps to fruit ripening and senescence has not been elucidated in lycopersicon esculentum ( tomato )
乙烯對植物的生長發育起重要的調控作用,隨著乙烯信號轉導研究的不斷深入,許多乙烯信號轉導
組分已被分離和鑒定,並在模式植物擬南芥中已建立乙烯信號傳遞過程中的核內級聯反應: ein3 erf1 ( erebps )病原相關蛋白,但在
番茄中還沒有建立ein3 erebps果實成熟相關基因的相似路徑。
The chloroplast shsp gene was screened from the cdna library of tomato flower by pcr strategy and confirmed by sequencing. but difference was found at 3 bases of the sequence from the reported in genbank. then, an integrated vector prok ii of the chloroplast shsp gene and nptii gene ( a kanamycin resistant gene ) with camv35s promoter was constructed and introduced into tomato mediated by agrobacterium tumefaciens lba4404. transgenic tomato were screened by their ability of growing on media containing kanamycin
本實驗採用pcr方法從
番茄花cdna文庫中克隆到葉綠體shsp基因,經測序證實與genbank中已發表的序列在編碼區相差2個堿基,其中一個堿基導致1個氨基酸的改變。將葉綠體shsp基因定向克隆于帶有
組成性表達啟動子camv35s的植物表達載體prok中,凍融法轉化農桿菌lba4404 ,利用葉圓盤法對
番茄進行ti質粒介導的遺傳轉化。
These results indicated that there existed tissue specificity with regard to accumulation of leerfl, leerf2 and pti4 mrna induced by aba and sa. the mrna abundance of leerf1, leerf2 and pti4 decreased gradually in the mature green tomato fruits as the treatment concentration of cacl2 increased, which indicated that ca2 + involved in the ethylene signal transduction
Cacl _ 2滲透處理綠熟期
番茄整果,隨著cacl _ 2處理濃度的增加, leerf1 、 leerf2 、 pti4基因表達逐漸減弱,表明鈣可調控乙烯信號轉導
組分。
Study on the methods on identifying the virulence of ralstonia solanacearum using tissue cultured tomatoes
青枯雷爾氏菌致病力的
番茄
組培苗鑒定方法研究
The long lake scenic area is composed of the mopan mountain, dushi mountain, long lake and penglai islet. looking at the far - reaching green mountains and beautiful lake, visitors will specifically enjoy its fresh atmosphere, secluded and elegant charms
長湖風景區由磨盤山獨石山長湖蓬萊島
組成,放眼四顧,只風山清水秀,清新幽雅,別有一
番情趣。
4. the wild tomato and nr mutant fruit discs treated with wound, nacl and peg to detect expression changes of le - acs gene family. the experiment showed that during the ethylene production under stress conditions, the le - acs2 gene expression maintained at a high level, different from system i ethylene production transition to system ii. this suggested that stress conditions changed the regulatory mechanism of le - acs2
傷、 nacl 、 peg處理普通型
番茄和nr突變體
番茄的
組織圓片研究le - acs基因家族的表達變化,結果表明:在脅迫乙烯生成過程中, le - acs2一直呈高水平表達,不存在如同系統向系統乙烯的轉化中le - acs基因家族的表達過渡,因此,脅迫條件下le - acs2基因的轉錄調控機制發生改變。
I thank the committee, most of whose members having been nominated by the faculties, student bodies, the university council and the convocation, and particularly professor ambrose king, the chairman of the committee, for all their efforts
委員會成員包括大學校董、師生和校友,多為各學院、學生
組織、校董會和校友評議會推選之代表。各委員殫精竭慮,經過多
番討論,凝聚共識,最後無異議通過報告書之修訂本,並沒要求將個人意見一併呈交。
Furthermore, the resistance to 3 - at of the dual reporter strains was tested on the sd / - fiis - ura plates containing 0, 10, 20, 30 mm 3 - at. as the 3 - at level increases, the size of the dual reporter strains got smaller and completely disappeared by 30 mm 3 - at
對兩種激素的應答比jerfz快速而又強烈,這可能與兩個基因啟動子中順式作用元件的
組成有關,同時也反映出兩者在
番茄體內的作用也可能有所不同。
The ian food group is made up of 3 production plants producing olives, tomato and asparagus
Ian食品集團由三家生產橄欖、番茄和蘆筍的工廠組成。