病毒增殖 的英文怎麼說

中文拼音 [bìngdúzēngzhí]
病毒增殖 英文
multiplication of virus
  • 病 : Ⅰ名詞1 （疾病; 失去健康的狀態） illness; sickness; disease; malum; nosema; malady; morbus; vitium...
  • 毒 : Ⅰ名詞1 （對生物體有害的性質或物質; 毒物） poison; toxin 2 （毒品） drug; narcotics 3 （姓氏） a s...
  • 殖 : 殖Ⅰ動詞（生息; 孳生） breed; grow; multiply Ⅱ名詞（姓氏） a surname
  • 病毒 : [醫學] virus; inframicrobe （濾過性）
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. In trpsin tolerance assay. this virus could resist to 1 % trpsis at 37 in an hour. in acid tolerance assay, this virus was resistant to ph3. 0 and ph5. 0 at 37 in 2 hours, and the average infection litre of the virus decreased little. in heat assay, at 50, the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree. among these conditions, when at 50 in 30 minutes. the average infection litre of this virus decreased over 2 tilre. and when al 50 in an hour, cpe of ihis virus disappeared. when time was set for an hour. but with processed in different temperature as 50 60 70, 80, the virus losl the multiplication capacity complelely. in biological assay, we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory. then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second. with f81 cell line, after passaged for 12 times continuously with low concentration of fcs. the virus could produce cpe. however, with vero cell line. the virus could not procuce any cpe after many passages. the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig, fowl and cavy. by neutrolizaion assay, dxmv could be identified as a kind of ccv

    理化學研究表明，該病毒為rna病毒，對氯仿、乙醚敏感；胰酶試驗中，經37 、 1小時處理的病毒，仍然能夠在貓源細胞fcwf細胞上生長，並且毒力基本保持不變；耐酸性試驗中，病毒分別在ph5 . 0和ph3 . 0經37作用2小時，毒力僅下降一個滴度；耐熱性試驗中，該病毒在恆定溫度50 ，設定不同時間，從5分鐘到150分鐘，毒力均有不同程度下降，其中， 50作用30分鐘，病毒平均滴度下降2個單位； 50 ， 60分鐘， cpe消失；恆定時間1小時，設定不同溫度（ 50 - 60 - 70 - 80 ） ，病毒在細胞上完全喪失增殖能力， cpe消失。生物學試驗，利用實驗室現有條件，選擇不同的細胞系對該病毒進行培養，發現該病毒對貓源細胞fcwf最敏感； mdck細胞次之； f81細胞經多次傳代，亦可出現cpe ；而vero細胞則不敏感。血凝試驗表明，該病毒對豬、雞、人及豚鼠的紅細胞均無血凝性。
  3. Detection and proliferation of hemorrhagic fever virus in chigger mites

    恙蟎體內出血熱病毒檢測增殖及其定位
  4. The mechanism enhancement of the optical brightener is not known. shapiro et al. postulated that selected brightener including m2r inhibit or alter the chitinous peritrophic membrane ( pm ), creating gaps in the membrane or gut lining and perhaps allowing more virions to pass from the gut lumen into the hemocoel

    光增白劑對桿狀病毒的增效作用的機理存在兩種推測一種觀點認為光增白劑是通過破壞圍食膜結構的完整性，促使更多的病毒粒子穿越圍食膜而發動感染的；另一種意見認為光增白劑能延遲中腸上皮細胞的脫落，促進病毒的復制繁殖。
  5. These germs not only include the herpes simplex viruses, which lead to cold sores and possibly genital herpes, but also the diseases responsible for chickenpox and " mono, " as well as several less well - known ailments

    這些微生物不僅包括那些引起感冒瘡和生殖器皰疹的單純皰疹病毒，而且包括那些會引發水痘和單核細胞增多癥等幾種少有的疾病。
  6. Further multiplication of the virus produced papules on the skin.

    病毒的進一步增殖使皮膚發生丘疹。
  7. Susceptible cells, when infected at a high multiplicity of infection with virus, produce many incomplete of defective viral particles.

    易感細胞感染一種能高度增殖的病毒時能產生許多不全的或缺損的病毒粒子。
  8. Infection of susceptible cells with a virus in many cases results in the multiplication of progeny virus.

    多數情況下，易感細胞感染病毒后，可導致子代病毒的增殖。
  9. The virus was purified by ultracentrifuge. according to the ns gene sequence published by genbank, one primer t - 1 was designed to amplify the cdna by reverse transcript. the other primers nsl - u / ns1 - 1 and ns2 - u / ns2 - 1. hns2 - u were designed to amplify the ns1, ns2 and hns2 gene

    本實驗用接種雞胚的病毒增殖方法獲得了a / chicken / mudanjiang / 0823 / 00 （ h9n2 ）分離株的大量增殖，增殖病毒經差速離心進行純化，經超迷離心濃縮。
  10. The research consist of four parts. the first part is multiplication, purification and electron microscope examination of the avian encephalomyelitis virus. a 1 : 5 dilution of isolate - nh937 of aev and control group of pbs were inoculated to susceptible 6 - day - old chickens embryos. respectively. after incubation for 10 days, the urinay vesicle liquid was collected. making a comparison the size of the chickens embryos between the test group and the control group, the results showed that the size of the control group is bigger than that of the test group. purified virions were examined under the electron microscope, the result revealed that there are a lot of virions and the aev - nh937 was multiplicated in embryos. the second part was seguence analysis of the genome of the aev - nh937. nine pairs of primers were designed according to published calnek vaccine strain of aev

    本研究共分四個部分：第一部分為aev的增殖，純化和電鏡觀察，用1 : 5倍稀釋的aev - nh937株和陰性對照pbs分別經卵黃囊接種於6dspf雞胚，繼續孵化10d后，收集尿囊液。比較接種組和健康對照組雞胚的大小，結果顯示，健康對照組雞胚明顯大於接種組。分離、提純aev ，把純化的病毒在電鏡下觀察，證明確有大量aev病毒粒子存在，說明aev在雞胚中成功擴增；第二部分是aev - nh937基因組的序列測定工作。
  11. The expression efficiency difference between ped5 and pcdhfrl, a vector utilizing cmv enhancer / promoter ( pcmv - ie ) for foreign protein production, was analyzed using human interferon - p ( ifn - ) gene and human secreted alkaline phosphatase ( seap ) gene as reporters. when analyzed in transient expression, ped5 showed a little more protein produciton than pcdhfrl. however, in continuous expression, when serum concentration was lessened to slow down cell proliferation, ped5 expressed 3. 1 times more reporter proteins than pcdhfrl, which implied that pef - io was less affected by cell cycle status in contrast to pcmv - ie, making ped5 a good expression vector for foreign protein production

    應用人-干擾素（ ifn - ）和人分泌型堿性磷酸酶（ seap ）基因作為報告基因，對含有巨細胞病毒即早期啟動子（ p _ （ cmv - ie ） ）的表達載體pcdhfr1和ped5表達外源蛋白的能力進行了比較，發現對于瞬時表達， ped5略好於pcdhfr1 ；在穩定表達中，通過降低血清濃度，使細胞增殖緩慢，這時ped5表達外源蛋白的能力較pcdhfr1高3 . 1倍。
  12. Biological activity was determined by egf dependent balb / c 3t3 cell line and with mtt colorimetric assay. extracts of the recombinant virus - infected and mock - infected cells, haemolymph of the recombinant virus infected and mock - infected silkworm larvae could all support the proliferation of balb / c 3t3 cell. this phenomena implied that there were some egf - like growth factors in the haemolymph of normal silkworm larvae, which could enhance the proliferation of the cell line

    用小鼠balb c3t3成纖維細胞和mtt法測定表達產物的促細胞增殖作用，發現重組病毒感染家蠶細胞72小時的胞內樣品與正常家蠶細胞裂解物，以及重組病毒感染4天的蠶血淋巴與正常蠶血淋巴均具有相似的促細胞增殖作用，甚至野生型病毒感染的細胞裂解物和蠶血淋巴也有一定的細胞促生長作用，提示家蠶系統本身可能含有能促進細胞生長、類似於egf的細胞因子。
  13. The changes of above show that the damage to the chicken embryo caused by the chicken parvoviruses were widly. the pathogeny of infectious stunting syndrom was prolifirated in cells nucleus, it was a dna virus

    以上變化說明了分離病毒對機體損傷的廣泛性。核內包涵體的出現進一步說明，雞傳染性生長障礙綜合征的病原在細胞核內增殖，為dna病毒。
  14. The expression product is so cheap and safety that its application foreground is great

    與滅活病毒相比既廉價，安全，又避免了病毒增殖的繁瑣，其應用前景可觀。
  15. In young chickens aev induces paralysis, ataxia and muscular dystrophy, while in older chickens, infection is usually subclinical, resulting in a decline in egg production and hatchability. infectivity was shown to remain unaffected by chloroform, low ph, pepsin, trypsin and deoxyribonuclease. magnesium cations were shown to stabilise preparations of the virus against heat inactivation. the buoyant density of virions are 1. 31g / ml. the diameter of the virion was estimated to be 22 to 30nm. the aev can be adapted to grow in chicken embryo. the inability of aev to grow effeciently in most cell cultures

    幼雞感染該病毒后，引起麻痹、頭頸震顫甚至共濟失調，而成雞常呈亞臨床感染或導致產蛋量和孵化率下降。病毒的感染性不受氯仿、低ph 、胃蛋白酶、胰酶和脫氧核糖核酸酶的影響，鎂離子可增強病毒對熱的穩定性，病毒的浮密度為1 . 31g ml ，直徑為22 - 30nm ，該病毒主要在雞胚中增殖，在大多數細胞培養物中不生長。
  16. Bovine viral diarrhea virus were propagated by mdbk cells. after five to six continuously and rapidly passing, the cells emerged evident cytopathic effect. then the infected cells and its solution were harvested

    Bvdvnadl株屬于致細胞病變型毒株，用mdbk細胞培養增殖bvdv ，經5 - 6次連續傳代至出現明顯的細胞病變。
  17. Occurrence of liver carcinoma is a synergistic action by multiple factors which include virus infection, carcinogen, oncogene activation, anti - oncogene inactivation, liver cell apoptosis, failure of proliferation regulation and so on

    肝癌的發生是一個多因素協同作用的過程，這個過程中包括病毒感染、致癌物的作用、癌基因的激活和抑癌基因的失活、肝細胞的凋亡和增殖調節失控等多因素多環節。
  18. The native jl94 isolate of porcine rotavirus ( rv ) was propagated in mal04 cel1 culture, and harvested after 48 hours

    本實驗在ma104細胞上培養增殖了豬輪狀病毒（ rv ）地方分離株jl94 ，並提取了病毒的雙股rna 。
  19. Viral multiplication cycle

    病毒增殖環
  20. The virus from the upper respiratory system exhibited a single amino acid change in one of the key proteins for amplification of influenza virus genes

    來自上呼吸道的病毒與流感病毒增殖的關鍵蛋白只相差一個氨基酸。
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