白果中毒 的英文怎麼說
中文拼音 [báiguǒzhōngdú]
白果中毒
英文
ginkgo poisoning- 白 : Ⅰ形容詞1 (似雪的顏色) white 2 (清楚; 明白; 弄明白) clear 3 (空的; 沒加他物的) pure; clear; ...
- 果 : Ⅰ名詞1 (果子) fruit 2 (事情的結局; 結果) result ; consequence 3 (姓氏) a surname Ⅱ動詞(吃...
- 毒 : Ⅰ名詞1 (對生物體有害的性質或物質; 毒物) poison; toxin 2 (毒品) drug; narcotics 3 (姓氏) a s...
- 白果 : [植物學] ginkgo; gingko; salisburia adiantifolia白果樹 gingko tree; gingko; maidenhair tree; seme...
-
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中,轉化大腸桿菌dh5菌株,篩選氨芐青霉素抗性菌落,提取質粒經酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原核表達載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導表達,收集菌液進行sds - page電泳、 westernblotting分析,結果表明, 3ab基因在大腸桿菌中成功表達,其表達產物為分子量33 . 5ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總蛋白量的26以上。In addition, we designed a test : the midgut was removed from spodoptera exigua larvae and was cut longitudinally into halves, the midgut epithelia were cleaned and digested with dispase ii, the pelleted cells in grace medium with 7 % fbs supplemented with 100units / ml penicillin, 100ug / ml streptomycin were cooled and then suspended in r18 - labelled virus
實驗結果表明,在幼蟲的病毒接種物中添加合適濃度的熒光增白劑( 0 . 01g . ml一』 )不會改變病毒在幼蟲中腸組織中的感染部位,卻可以提高幼蟲對病毒的敏感性,增加了病毒毒力。G gene of rabies virus m, the of two main regions ( about 1000nt ), ranging from 3161nt to 4162nt and ranging from 4012nt to 4863nt of glycoprotein gene of rabies virus strain m, isolated from mouse in he nan, china were amplified by reverse transcriptase - polynerase chain reaction ( rt - pcr ) in order to complete glycoprotein gene of strain m. these regions were sequenced by the produce of pcr directly. comparison and analysis of nucleotide sequence and amino acid sequence deduced with that of other strains published was performed by computer with dnasisv 2. 5demo software
本研究對我國河南某地野鼠體內分離的狂犬病野毒株mrv基因的3161位? 4162位( 1001個堿基)和4012位? 4863位( 851個堿基)片段進行了反轉錄pcr擴增和序列測定,得到mrv的糖蛋白基因全序列,用dnasisv2 . 5demo分析軟體,與已發表的代表性毒株g基因全序列進行核苷酸和氨基酸序列的比較分析,結果表明在同一基因型中, mrv和國際標準攻毒株cvs的同源性最高( 96 . 5 ) ,和中國減毒株ctn的同源性最低( 79 . 8 ) 。Abstract : the effects of terbium ion on the conformation of calmodulin and on the interaction between calmodulin and melittin have been studied by the endogenous fluorescent spectrometry of calmodulin and melittin, and the sensitized fluorescent spectrometry of terbium ion, respectively. the results show that terbium ions have a tight binding site in the i and ii metal - binding sites of calmodulin. the conformation of calmodulin induced by terbium ion can bind melittin and transfer the tryptophane residue of melittin to a relatively hydrophobic environment, while the binding of melittin to calmodulin produces effect on the binding orders of terbium ion in camodulin. results from ft - ir spectrometry have revealed that upon binding of lanthanum ion, apo - calmodulin undergoes a conformational change with the increase of - helix content and the decrease of - sheet content. melittin ' s binding to calmodulin has no effect on its conformation induced by the binding of lanthanum ion to calmodulin
文摘:分別用鈣調蛋白和蜂毒素的內源熒光光譜以及鋱離子的敏化熒光光譜考察了鋱離子對鈣調蛋白構象變化以及對鈣調蛋白與蜂毒素相互作用的影響.結果表明,鋱離子首先結合在鈣調蛋白的第和第位點,鋱離子不影響鈣調蛋白與蜂毒素的相互作用,蜂毒素與鈣調蛋白作用后不影響鋱離子在鈣調蛋白上的鍵合順序.傅里葉變換紅外光譜結果表明三價的鑭離子與鈣調蛋白作用使鈣調蛋白的螺旋結構增加,折疊結構減少,與鈣離子對它的二級結構影響相類似.稀土離子在鈣調蛋白-蜂毒素復合體系中主要與鈣調蛋白作用The activity of apx was also decresed after sa and vd - toxin treatment. the activity of cat was decreasded more obviously. in the vd - toxin infected arabidopsis, no also restrained the activity of cat and decreased the level of cat3 mrna. the results suggested that h2o2 acted as a signal to involve the defense reactions which is induced by sa and no in arabidopsis
No在毒素與擬南芥的互作過程中也表現出對cat的抑制作用,結果表明, h _ 2o _ 2可能作為信號分子參與了sa和no調控的擬南芥的防衛反應, sa和no或許在轉錄和蛋白水平上通過對cat的抑制作用而促進h _ 2o _ 2的積累。Expression of apple chlorotic leaf spot virus coat protein gene in e. coli
蘋果褪綠葉斑病毒庫爾勒香梨分離物外殼蛋白基因在大腸桿菌中的表達Strawberry brighten extract : natural fruit strawberry extract contains abundant lactic acid, effectively expel toxin from deep skin cells, eliminate dim complexion, leave skin fair and transparent
草莓煥膚精華:天然水果草莓萃取精華液含豐富的乳酸成分,有效排除肌膚深層細胞中的毒素,一掃皮膚的晦暗,使皮膚回復通透白皙的質感。Functions : this product contains “ antifungal active protein ” with optimal and strong sterilization effect, which could strengthen the immunity of body fluid, activate the macrophage, strengthen the phagocytosis ability of macrophage, strengthen the body immunity, restrain the growth, pervasion and transfer of abnormal cells, the product has strong sterilization effect and could strengthen the disease resistance ; it is remarkably effective in improving the immunity and improving the infirm constitution of pets ; the amino acid content and composition in the product are moderate and rational, with the characteristics of strong palatability, nutrition balance and immune element abundance, etc
功能:本產品中擁有極佳強烈殺菌作用的「抗菌活性蛋白」 ,能增強體液免疫功能,活化巨噬細胞,增強其吞噬能力,可增強機體免疫力,抑制非正常細胞生長、擴散和轉移,具有強烈的殺毒作用,增強抗病性;對提高寵物免疫力,改善虛弱體質有顯著效果;其中氨基酸含量適中、組成合理,具有適口性強、營養均衡和免疫物質豐富等特點。The cloning cdna fragment was extracted from positive clones and sequenced. the results showed that the cdna fragment was 816bp in size, encoding a protein which included 272 amino acids. the sequence homology analysis was carried out via the software blast 2. 0 network service in the four large databases - genbank, embl, ddbj, pdb, which had recorded 1 337 978 nucleotide and protein sequences. the results of the analysis indicated that the nucleotide homologous rates between the rubber tree etr and 15 recorded etrl of other plants ( mango, passion fruit, persia plum, strawberry, grape. . etc ) were 75 % - 80 % ; the protein homologous rates between the rubber tree etrl and these recorded etrl genes were 90 % - 95 %. from the results mentioned above, we could confirm that the cdna of rubber tree etrl had been cloned
從陽性克隆子中提取克隆片段,經序列測定分析,結果表明,克隆片段的cdna大小為816bp ,編碼的蛋白質包含272個氨基酸。基因序列通過blast2 . 0networkservice軟體對genbank , embl , ddbj , pdb四個大型數據庫中記錄的1337978條核酸和蛋白質序列進行序列相似性檢索,結果表明與芒果、一西番蓮、波斯梅、草毒、葡萄、西洋梨等15種已報道的植物的etrl基因cdnag的同源率為75 88 ;蛋白質氨基酸序列的同源率為90 95 ,表明本研究確實克隆到了橡膠樹etri基因的cdna序列。 4At first, 1. 67 u g per well mcab all was coated on three wells of a plate, and then 1. 5 x 1011 phage virion was diluted and added, after incubating with the target, wash away unbound phage by tbst ( 0. 1 % tween - 20 ), the bound phage was eluted with ph 2. 2 tris - gly buffer and amplified, the specially bound phage was enriched by taking through addition binding / amplification cycles. ln the following cycles, the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all, collecting and titering the washing phage of last time and output phage in each round, the selective ratio and the false positive rate of each round were worked out, the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective. after 4 rounds of panning, 11 phage clones were selected after competitive - ellsa, the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced, a clear consensus binding sequence emerged
在本實驗中,利用隨機12肽庫對抗豬瘟病毒( classicalswinefeverviruscsfv )糖蛋白me2的單抗a11進行表位篩選,經過四輪篩選以後,隨機挑取11個克隆作競爭- elisa檢測,結果表明,所挑11個克隆中,有9個克隆能對me2蛋白和a11反應產生抑制作用,抑制率最高可達64 ; dna測序以後經過dnastar軟體分析,發現它們的核心序列為anwralsl ,該核心序列與豬瘟病毒e2蛋白的28 - 35位氨基酸ttwkeysh具有同源性;夾心- elisa檢測和western - blotting試驗均證明所挑陽性克隆能被a11所識別;人工合成含核心序列的多肽經間接elisa試驗證實,也能被a11識別。Results we construct recombinant angiostatin baculovirus with a high virus titer ( 2 + 108 pfu / ml ) successfully. recombinant angiostatin was effectively expressed in insect cells ( sf9 ) as 53 kd fusing protein and its expression level was about 90 % of insect cellular total soluble proteins. the recombinant angiostatin protein could inhibit endothelial cell proliferation in vitro with ic50 value of 2
實驗結果我們成功地構建了滴度高達2x10 『 pm llil的angiostatin重組? 2 ?桿狀病毒,並在昆蟲細胞sffi中高效表達了分子量為53kd的an giostatin重組蛋白,重組angiostatin蛋白不僅在體外顯著抑制內皮細胞的生長, k 。The protein product of meq gene was highly expressed in the nuclei of recombinant baculovirus infected sf9 cells when using an anti - meq monoclonal antibody ( mcab ) 23b46 to run the immunofluorescence assay ( fa ) ; the expression quantity and if staining patterns differed with different times post - infection ( pi ). the results of western blotting and immunoprecipitation test showed there were two specific bands around 60 kd. the results of the study demonstrated that the baculovirus / insect cell system is effective to be used to express nuclear protein of virus
結果發現:本表達系統產生的meq蛋白可被重組痘病毒表達的meq制備的單抗23b46所識別;在感染細胞中, meq蛋白僅局限於細胞核內,而且隨著感染后( pi )時間的增加,具有從核質向核仁和核膜轉移的趨向; w已stemblot和免疫沉澱試驗均證實重組桿狀病毒感染細胞裂解物中出現有兩條大小約為60kd的特異帶。Further sequence analysis show that only 6 base pairs of nucleotide and 2 amino acids are different between them. the homological cry3aa gene was expressed in escherichia coli. and the expressed products which contain a fused peptide of 66 - 97 kilo - dalton was observed by means of sds - page
生物活性測定結果表明該菌株對榆藍葉甲( pyrrhaltaaenescens ( fairmaire ) )和光肩星天牛等鞘翅目昆蟲具有較高的毒力,因此初步確認該菌株屬于cry3類; ( 2 )發現該菌株中編碼毒蛋白的基因位於質粒上,並且已經成功地克隆到該基因。Heroin peddling were carried out around canton road and waterloo road, and the neighbouring tai fat store and the first floor of the tai loy restaurant were used for meetings, dispensing bribes, and as a general command centre. the rear lanes surrounding the area and alleys inside the fruit market were the preferred drug transaction points. occasionally the syndicate would hide small packets of " white powder " in the counter on the first floor of the tai loy to be picked up by the " field mines " - drug carriers who solicited business in the streets
他們盤據廣東道與窩打老道一帶,利用相鄰的大發士多和大來酒樓大來二樓作為通訊及聯絡中心,附近的橫巷和果欄就是毒品交易的場地他們間中也會將小量白粉存於大來二樓的櫃臺,方便地雷即販毒集團內專責帶毒的計取用。136 of 169 enterprises of formula feed and concentrated feed producing were measure up, the rate of fitness was 80. 47 %. 314 of 352 samples of formula feed and concentrated feed products were measure up, the rate of fitness was 89. 20 %. the main problems of these products were content of crude protein, crude ash and aflatoxin bl not in accordance with its standard
結果表明:在抽查的169家我省配合飼料和濃縮飼料生產企業中,產品全部合格的136家,企業合格率為80 . 47 ;在抽查的352批次樣品中,合格314批次,樣品合格率為89 . 20 。主要問題是粗蛋白質、粗灰分、黃麴黴毒素b _ 1等項目不合格。The levels of metallothionein synthesized in liver of the mice induced by different stress factors including trauma, starvation, zinc ( zn ) nutriment, cadmium ( cd ) poison, both zn nutriment and cd poison and different treating ways which were designed according to the time - effect and dosage - effect were detected by atomic absorb mean and sulfydryl saturation mean respectively
用鎘飽和法及巰基顯色法兩種方法檢測小白鼠肝臟mt的量,研究不同應激因子誘導小白鼠肝臟mt合成的對應情況,結果表明: 1鎘常規毒理實驗中,鎘對小白鼠腹腔注射的半致死劑量為2 . 12 ( 2 . 67 1 . 71 ) mg / kg 。After bacterium cells were treated by formaldehyde, surface proteins of gram - negative bacterium e. coli k12 were eluted little, but it had no influence on adsorption of snake venom proteins to bacterium cells. treatment by formaldehyde had little influence on snake venom absorption to and deabsorption from gram - positive bacterium streptococcus mutans, but absorbance of interaction protein with molecular weight of 47kda only adsorbed by streptococcus aureus was greatly decreased
結果表明,滅活后的革蘭氏陰性菌( e . colik12 )再與蛇毒相互作用時,洗脫下來的蛋白中菌體蛋白含量明顯減少,但不影響對作用蛋白的吸附;甲醛滅活對革蘭氏陽性菌( s . mutans和s . aureus )影響不大,只有s . aureus吸附蛋白中47kda的蛋白吸附量減少,不會影響作用蛋白中其它蛋白的吸附。In recent years, china has from the northeast changbai mountain snake and purified snake venom " ahylysantinfarctase " and " bolt - on " used for the treatment of thrombosis obliterans, and received good results, with no obvious side effects
近年,我國先後從東北蛇島和長白山蝮蛇蛇毒中提純出「抗栓酶」和「清栓酶」 ,用來治療血栓閉塞性脈管炎,獲得良好效果,且無明顯副作用。The biological characteristics and toxicity of russula subnigricans hongo was studied for the first time from ecology and morphologic characteristics and histology, the orthogonal experiment of the optimum culture condition, the analysis of components, apoptosis of the cells from little white rat liver and kidney induced by extract of russula subnigricans hongo, to the histopathologic changes observation of little white rat liver and kidney through ecological observation, light microscopy and scanning electron microscopy, reversed - phase high performance liquid chromatography, agarose gel electrophoresis, transmission electron micioscopy. the result showed as below : based on ecological observation of russula subnigricans hongo, its ecological environment was investigated in order to simulate its ecological environment when they are cultivated
利用菌種分離技術、光鏡技術、電鏡技術、高效液相色譜技術、毒理實驗技術、電泳方法等對亞稀褶黑菇( russulasubnigricanshongo )的生物學特性和毒性機理進行了研究,主要包括以下內容:亞稀褶黑菇的生態學和組織學研究、菌種分離培養、掃描電鏡觀察、成分分析、粗毒液誘導小自鼠肝腎細胞凋亡,小白鼠中毒后肝腎細胞透射電鏡觀察,研究結果如下: 1Finally, in order to investigate whether these two virus are resistant to complement attack, and whether the resistance were mediated by incorporating cellular complement regulators of cd55, cd59 into their out membrane, complement lysis assay and plaque reduction assay were carried out respectively to find the lysis or neutralization activity of complement to hiv and eev under the condition of with or without the cd55 and cd59 blocking antibody
將這兩種病毒分別暴露於人血清補體后,發現在有cd55 、 cd59中和性抗體存在的情況下,補體溶解hiv病毒的效率明顯增加; eev病毒的感染活性在空斑實驗中也明顯降低,結果與這兩種蛋白在病毒表面( eev )存在的事實相符,提示病毒表面的cd55分享友人